RFLP mapping of the vernalization (Vrn1) and frost resistance (Fr1) genes on chromosome 5A of wheat

Abstract

A population of single chromosome recombinant lines was developed from the cross between a frost-sensitive, vernalization-insensitive substitution line, 'Chinese Spring' (Triticum spelta 5A) and a frost-tolerant, vernalization-sensitive line, 'Chinese Spring' ('Cheyenne' 5A), and used to map the genes Vrn1 and Fr1 controlling vernalization requirement and frost tolerance, respectively, relative to RFLP markers located on this chromosome. The Vrn1 and Fr1 loci were located closely linked on the distal portion of the long arm of 5AL, but contrary to previous observations, recombination between them was found. Three RFLP markers, Xpsr426, Xcdo504 and Xwg644 were tightly linked to both. The location of Vrn1 suggests that it is homoeologous to other spring habit genes in related species, particularly the Sh2 locus on chromosome 7 (5H) of barley and the Sp1 locus on chromosome 5R of rye.

Genetic analysis of some flowering time and adaptive traits in wheat

“…The known maps for the three group 5 chromosomes are given in Figure 3. This is based upon the earlier results with 5 A and 5D and recently published mapping data for chromosome 5 A (Galiba et al, 1995;Devos et al, 1995). These indicate that the three Vrn genes are each located in similar positions on their respective chromosomes.…”

Segregation analysis of heading traits in hexaploid wheat utilizing recombinant inbred lines

“…Segregation analysis of heading in wheat C Shindo et al tion (Figure 2a), although three RFLP markers, BCD450, PSR426 and CDO504, had been reported as the tightlinked markers to Vrn-A1 (Galiba et al, 1995;Kato et al, 1998). On the other hand, a large LOD peak (Po0.01) for vernalization requirement was detected in the 15.8 cM Xcdo1326b-Xgwm116 interval on 5B (Figure 2b).…”

“…For the vernalization requirement, three major genes, Vrn-A1, -B1 and -D1, have been well characterized and are reported to be located on the long arms of chromosomes 5A, 5B and 5D, respectively (Law et al, 1976;Maystrenko, 1980;Scarth and Law, 1983;Hoogendoorn, 1985a;Worland and Law, 1986;Galiba et al, 1995;Nelson et al, 1995;Korzun et al, 1997;McIntosh et al, 1998). For photoperiod sensitivity, three major genes, Ppd-A1, Ppd-B1 and Ppd-D1 (initially Ppd3, Ppd2 and Ppd1, respectively), also thought to be homoeoallelic, are located on chromosomes 2A, 2B and 2D, respectively (Welsh et al, 1973, Law et al, 1978, Scarth and Law, 1983.…”

Molecular characterization of the allelic variation at the VRN-H2 vernalization locus in barley

“…Comparative RFLP mapping between wheat and barley has shown that the VRN-1 and VRN-2 genes from these species are orthologous. The VRN-H1 gene has been mapped on the long arm of chromosome 5H in barley, in the same region as the VRN-A1, VRN-B1, and VRN-D1 in wheat (Dubcovsky et al 1998;Barrett et al 2002;Galiba et al 1995;Laurie et al 1995;Iwaki et al 2002;Yan et al 2003). The VRN-H2 gene has been mapped in the distal part of chromosome arm 4HL in barley (Laurie et al 1995) and in the distal part of chromosome arm 5A m L in diploid wheat, in a region translocated from chromosome arm 4A m L (Devos et al 1995;Laurie et al 1995;Dubcovsky et al 1998;Yan et al 2004).…”