RFLP and Cytogenetic Evidence on the Origin and Evolution of Allotetraploid Domesticated Peanut, Arachis hypogaea (Leguminosae)

Kochert, Gary; Stalker, H. T.; Gimenes, Marcos Aparecido; Galgaro, Leticia; Lopes, Catalina Romero; Moore, Kathryn L.

doi:10.2307/2446112

Cited by 193 publications

(166 citation statements)

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“…Recently, it was also found in UV-treated leaves of ten wild species of the section Arachis (Lopes et al, 2013), including A. duranensis and A. ipaënsis, the progenitors of the cultivated peanut (Kochert et al, 1996).…”

Section: Introductionmentioning

confidence: 95%

Research Article Coupled transcript and metabolite identification: insights on induction and synthesis of resveratrol in peanut, wild relatives and synthetic allotetraploid.

et al. 2017

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ABSTRACT.Resveratrol is an antioxidant that is a promising antitumoral, cardioprotective and neuroprotective agent. It has been found in a restricted number of plants including peanut (Arachis hypogaea L.) and its wild relatives. The objective of this study was to understand the relationship between resveratrol content and the expression of putative resveratrol synthase genes in four Arachis genotypes. Two diploids and two tetraploid were analyzed. Contents of resveratrol on non-and UV-treated leaves were estimated using HPLC. Resveratrol synthase (RS) was analyzed using RT-qPCR with primers developed in this study. Sequences of six Arachis species were amplified using two degenerated primer pairs that were designed based on Arachis and general RS available at GenBank. Those sequences were used to qPCR primers design. Test and control leaves were collected from plants cultivated in greenhouse and three biological replicates were evaluated for each genotype. The synthesis of resveratrol in leaves was induced by treatment with UV for 2.5 h. All genotypes studied synthesized resveratrol. Concentrations ranged from 193.66 µg/g in synthetic allotetraploid to 371.97 µg/g in A. duranensis. Natural and induced allotetraoploids showed lower levels of resveratrol than their diploid parents. Untreated samples did not produce significant amounts of resveratrol. The analysis of resveratrol content and levels of RS mRNA allowed the identification of one gene induced by the UV treatment. The data showed different amounts of RS in the different genotypes suggesting early and late response to the UV induction in the different species. The understanding of the variation found among species will help to identify species that have high resveratrol content and their ideal pos-induction times. This also will allow analysis of other tissues where high levels resveratrol would be very important, such as in seeds.

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Section: Introductionmentioning

confidence: 95%

Research Article Coupled transcript and metabolite identification: insights on induction and synthesis of resveratrol in peanut, wild relatives and synthetic allotetraploid.

et al. 2017

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“…The primers used in this study are shown, and the numbers in parentheses indicate the positions of the primers relative to the translation start site (0). The PCRampli®ed regions mentioned in the text are also indicated 2n 2x 20) and A. ipaensis (BB genome; 2n 2x 20) (Kochert et al 1996). Primers used to amplify Region 1 (788 bp) were F0a and R3 for A. duranensis, and F0 and R3 for A. ipaensis (Table 1, Fig.…”

Section: Sequence Comparison Among Oleoyl-pc Desaturases From Tetraplmentioning

confidence: 99%

The high oleate trait in the cultivated peanut [Arachis hypogaea L.]. I. Isolation and characterization of two genes encoding microsomal oleoyl-PC desaturases

Jung¹,

Swift²,

Sengoku³

et al. 2000

Mol Gen Genet

138

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Plant oils rich in oleate are considered superior products compared to oils rich in polyunsaturated fatty acids. Peanut (Arachis hypogaea L.) is one of the major oilseed crops, and high oleate mutant varieties with as much as 85% oleate have been reported. We examined the possibility that this mutant phenotype resulted from reduction in the activity or the transcript level of microsomal oleoyl-PC desaturase. Two independently generated high oleate mutants, M2-225 and 8-2122, and their partially isogenic lines with a normal oleate phenotype were used in this study. Two cDNA sequences coding for microsomal oleoyl-PC desaturases, ahFAD2A and ahFAD2B, have been isolated from the developing peanut seed with a normal oleate phenotype. Cultivated peanut is an allotetraploid, and sequence comparisons with the genes from the putative diploid progenitor species suggested that ahFAD2A and ahFAD2B are non-allelic, but homeologous genes originating from two different diploid species. Northern analysis showed that the transcripts of oleoyl-PC desaturases are highly abundant in both normal and high oleate peanut seeds in the second stage of development. Differential digestion of the RT-PCR products revealed a restriction site polymorphism between ahFAD2A and ahFAD2B, and allowed us to examine the level of transcript expressed from each gene. The results indicate that ahFAD2A is expressed in both normal and high oleate peanut seeds, but the steady state level of the ahFAD2B transcript is severely reduced in the high oleate peanut varieties. These data suggested that the reduction in ahFAD2B transcript level in the developing seeds is correlated with the high oleate trait.

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“…The world annual production of peanut is about 38 million tons (Bertioli et al 2011). Cultivated peanut is allotetraploid (AABB, 4n=4x=40) which originated from a single hybridization event between AA and BB wild species and subsequently underwent spontaneous genome duplication (Stalker and Simpson 1995;Moretzsohn et al 2013;Kochert et al 1996;Ferguson et al 2005;Freitas et al 2007;Gregory and Gregory 1979;Hammons 1982;Knauft and Gorbet 1995). Peanut gynophore is a specialized and unique organ that carries and eventually pushes the fertilized ovules into the soil in order for seed and fruit development to occur (Xia et al 2013;Moctezuma 2003).…”

Section: Introductionmentioning

confidence: 99%

Identification and expression dynamics of three WUSCHEL related homeobox 13 (WOX13) genes in peanut

Wang

Cui

et al. 2015

Dev Genes Evol

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WUSCHEL-related homeobox (WOX) genes play key roles in plant stem cell maintenance and development. WOX genes showed specific expression patterns which are important for their functions. WOX13 subfamily genes as the ancestor genes of this family were less studied in the past. In this study, we cloned three Arachis hypogaea (peanut) WOX13 (AhWOX13) subfamily genes from peanut: WOX13A and WOX13B1, 2. WOX13B1 encoded a same protein as WOX13B2, and there were only two-base difference between these two genes. Differential expression patterns were observed for these three AhWOX13 subfamily genes in different tissues and developmental stages. Phylogenic trees analysis showed that these AhWOX13 subfamily genes were the most conserved WOX genes and belonged to the ancient clade of WOX family. This was also supported by the conserved motif analysis. Selective pressure analysis showed that the WOX family genes mainly underwent weak purifying selection (ω = 0.58097), while many positive mutations accumulated during the evolution history. Under the purifying selection, gene duplication event and loss of duplicated gene play important roles in the expansion and evolution of WOX family.

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RFLP and Cytogenetic Evidence on the Origin and Evolution of Allotetraploid Domesticated Peanut, Arachis hypogaea (Leguminosae)

Cited by 193 publications

References 0 publications

Research Article Coupled transcript and metabolite identification: insights on induction and synthesis of resveratrol in peanut, wild relatives and synthetic allotetraploid.

Research Article Coupled transcript and metabolite identification: insights on induction and synthesis of resveratrol in peanut, wild relatives and synthetic allotetraploid.

The high oleate trait in the cultivated peanut [Arachis hypogaea L.]. I. Isolation and characterization of two genes encoding microsomal oleoyl-PC desaturases

Identification and expression dynamics of three WUSCHEL related homeobox 13 (WOX13) genes in peanut

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