Abstract-Due to cost-effectiveness and easy-deployment, RFID location systems with passive floor tags are commonly utilized into many industrial fields, particularly in the emerging environment of the internet of things (IoT). High accuracy and precision are key demands for these location systems. Numerous studies have attempted to improve localisation accuracy and precision by using either dedicated RFID infrastructures or advanced localisation algorithms. But these studies on improving RFID location systems mostly consider utilization of novel RFID localisation solutions rather than optimization of their utilization. Practical use of these solutions in industrial applications can lead to increased cost and deployment difficulty of RFID system. This paper attempts to investigate how accuracy and precision in passive RFID location systems are impacted by RFID infrastructures and localisation algorithms. A general experimental based investigation strategy, RFID-Loc, is designed for analyzing and evaluating the factors that impact the performance of a passive RFID location system. By experimenting a case study on passive HF RFID location systems with this strategy, it is discovered that (1) RFID infrastructure is the primary factor determining the localisation capability of a RFID location system. (2) Localisation algorithm is capable of improving localisation accuracy and precision, but limited by the primary factor. A discussion how to efficiently improve localisation accuracy and precision in passive HF RFID location systems is given.