RF plasma-enhanced conducting Polymer/W5O14 based self-propelled micromotors for miRNA detection

Çoğal, Gamze Çelik; Karaca, Gözde Yurdabak; Uygun, Emre; Kuralay, Filiz; Öksüz, Lütfi; Remškar, Maja; Öksüz, Ayşegül Uygun

doi:10.1016/j.aca.2020.07.010

Cited by 15 publications

(19 citation statements)

References 59 publications

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“…60 The speed of Au/Pt micromotors dramatically decreased from 73 to 31 μm/s after FAM-ssDNA probe incubation, resulting from the hybridization process that occurred on the outer surface of the ssDNA probe-modified micromotors. 37,40,61 Video S2 shows the trajectories of bare Au/Pt micromotors and FAM-ssDNA@Au/Pt micromotors w/o SAW and with SAW in the presence of 1% SDS, respectively. In the next step, the effect of acoustic treatment time (1, 3, 5, and 10 min) onto fluorescence intensities of FAM-ssDNA@ Au/Pt was studied at a constant acoustic streaming power (1 dBm) in the presence of 1% SDS (Figure 6).…”

Section: Resultsmentioning

confidence: 99%

“…The fluorescence signals and speeds of micromotors were determined after 30 min of hybridization time. 37,62 Hybridization is known as a powerful tool that can define the expression level of specific miRNA in cells or tissues. Nano/micromotor-based miRNA detection is also based on fluorescence quenching capacity and highly efficient delivery capability properties.…”

Section: Resultsmentioning

confidence: 99%

“…The incubation time was chosen according to our previous works. 37 The miRNA-21 detection protocol depends on the duplex formation (hybridization). The synthetic target miRNA-21 (100 nM) solution was obtained in 20 mM Tris-HCl buffer (pH 7.4) containing MgCl (5 mM), NaCl (100 mM), and KCl (5 mM) and then was immersed in the probe FAM-ssDNA@Au/Pt micromotor solution (20 μL) for 30 min of hybridization time.…”

Section: Imaging and Propulsionmentioning

confidence: 99%

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Fluorescence Detection of miRNA-21 Using Au/Pt Bimetallic Tubular Micromotors Driven by Chemical and Surface Acoustic Wave Forces

Çoğal

Das

Karaca

et al. 2021

ACS Appl. Bio Mater.

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In this study, surface acoustic wave (SAW) systems are described for the removal of molecules that are unbound to micromotors, thereby lowering the detection limit of the cancer-related biomarker miRNA-21. For this purpose, in the first step, mass production of the Au/Pt bimetallic tubular micromotor was performed with a simple membrane template electrodeposition. The motions of catalytic Au/Pt micromotors in peroxide fuel media were analyzed under the SAW field effect. The changes in the micromotor speed were investigated depending on the type and concentration of surfactants in the presence and absence of SAW streaming. Our detection strategy was based on immobilization of probe dye-labeled single-stranded probe DNA (6-carboxyfluorescein dye-labeled-single-stranded DNA) to Au/Pt micromotors that recognize target miRNA-21. Before/after hybridization of miRNA-21 (for both w/o SAW and SAW streaming conditions), the changes in the speed of micromotors and their fluorescence intensities were studied. The response of fluorescence intensities was observed to be linearly varied with the increase of the miRNA-21 concentration from 0.5 to 5 nM under both w/o SAW and with SAW. The resulting fluorescence sensor showed a limit of detection of 0.19 nM, more than 2 folds lower compared to w/o SAW conditions. Thus, the sensor and behaviors of Au/Pt tubular micromotors were improved by acoustic removal systems.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Imaging and Propulsionmentioning

confidence: 99%

See 1 more Smart Citation

Fluorescence Detection of miRNA-21 Using Au/Pt Bimetallic Tubular Micromotors Driven by Chemical and Surface Acoustic Wave Forces

Çoğal

Das

Karaca

et al. 2021

ACS Appl. Bio Mater.

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“…[63] Polymeric micro or nanomotors based biosensors typically depend on mutual interaction, such as DNA hybridization, antibody-antigen combination, and enzyme-substrate recognition. [9,64] An example was a stomatocyte structural color-barcode micromotor reported by Cai et al in Figure 12. [65] The stomatocyte-shaped micromotor driven by catalysis was fabricated from platinum-dispersed hydrogel with the assistance of stomatocyte photonic crystal (PhC).…”

Section: Biosensingmentioning

confidence: 99%

“…For example, a polymer/W 5 O 14 micromotor with impressive biomarker responsive performance was constructed via radio frequency rotating plasma polymerization process, which was novel for motorfabrication. [9] In this review, both traditional and more recent fabrication methods of polymer-based micro or nanomotors will be covered, along with interesting biomedical applications. With typical works presented and discussed, we hope to point out current challenges and future prospects of the polymeric micro or nanomotors.…”

Section: Introductionmentioning

confidence: 99%

Polymeric Micro/Nanomotors and Their Biomedical Applications

Liang

Peng

2021

Adv Healthcare Materials

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Since their naissance in the 2000s, various micro or nanomotors with powerful functions have been proposed. Among them, polymer-based micro or nanomotors stand out for the easy processing and facile functionalization, holding immense potential for bioapplications. In this review, fabrication of polymer-based micro or nanomotors and their applications in biomedical areas are covered. Classic manufacturing approaches as well as cutting-edge techniques are discussed with representative works highlighted. Current challenges and future prospects are presented in the hope of pointing new research directions to facilitate practical translations of micro/nanomotors.

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Micro‐ and Nanorobots Meet DNA

Urso

Pumera

2022

Adv Funct Materials

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DNA, the well‐known molecule that carries the genetic information of almost all forms of life, represents a pivotal element in formulating intelligent and versatile micro/nanorobotic systems. DNA‐functionalized micro/nanorobots have opened new and exciting opportunities in many research areas due to the synergistic combination of self‐propulsion at the micro/nanoscale and the high specificity and programmability of DNA interactions. Here, their designs and applications are critically reviewed, which span from the use of DNA as the fuel to chemotactically power nanorobots toward cancer cells to DNA as the main building block for sophisticated phototactic biorobots, DNA nanodevices to self‐monitor microrobots’ activity status, DNA and RNA sensing, nucleic acids isolation, gene therapy, and water purification. The perspective on future directions of the field is also shared, envisioning DNA‐mediated reconfigurable assemblies of nanorobotic swarms.

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RF plasma-enhanced conducting Polymer/W5O14 based self-propelled micromotors for miRNA detection

Cited by 15 publications

References 59 publications

Fluorescence Detection of miRNA-21 Using Au/Pt Bimetallic Tubular Micromotors Driven by Chemical and Surface Acoustic Wave Forces

Fluorescence Detection of miRNA-21 Using Au/Pt Bimetallic Tubular Micromotors Driven by Chemical and Surface Acoustic Wave Forces

Polymeric Micro/Nanomotors and Their Biomedical Applications

Micro‐ and Nanorobots Meet DNA

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