Revolutionize Genetic Studies and Crop Improvement with High-Throughput and Genome-Scale CRISPR/Cas9 Gene Editing Technology

Yang, Ning; Wang, Rongchen; Zhao, Yunde

doi:10.1016/j.molp.2017.08.001

Cited by 22 publications

(9 citation statements)

References 11 publications

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“…Additionally, genes involved in vemurafenib resistance were found in a melanoma model, including previously identified genes NF1 and MED12, and the newly identified NF2, CUL3, TADA2B, and TADA1. At present, genome-wide CRISPR-Cas9 screening libraries have been adopted to screen for drug resistance, virulence, and tumor suppressor genes in multiple cell lineages and primary cells [24][25][26][27][28][29][30][31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Screening Genes Promoting Exit from Naive Pluripotency Based on Genome-Scale CRISPR-Cas9 Knockout

Yang

Kuang

et al. 2020

Stem Cells International

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Two of the main problems of stem cell and regenerative medicine are the exit of pluripotency and differentiation to functional cells or tissues. The answer to these two problems holds great value in the clinical translation of stem cell as well as regenerative medicine research. Although piling researches have revealed the truth about pluripotency maintenance, the mechanisms underlying pluripotent cell self-renewal, proliferation, and differentiation into specific cell lineages or tissues are yet to be defined. To this end, we took full advantage of a novel technology, namely, the genome-scale CRISPR-Cas9 knockout (GeCKO). As an effective way of introducing targeted loss-of-function mutations at specific sites in the genome, GeCKO is able to screen in an unbiased manner for key genes that promote exit from pluripotency in mouse embryonic stem cells (mESCs) for the first time. In this study, we successfully established a model based on GeCKO to screen the key genes in pluripotency withdrawal. Our strategies included lentiviral package and infection technology, lenti-Cas9 gene knockout technology, shRNA gene knockdown technology, next-generation sequencing, model-based analysis of genome-scale CRISPR-Cas9 knockout (MAGeCK analysis), GO analysis, and other methods. Our findings provide a novel approach for large-scale screening of genes involved in pluripotency exit and offer an entry point for cell fate regulation research.

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Section: Introductionmentioning

confidence: 99%

Screening Genes Promoting Exit from Naive Pluripotency Based on Genome-Scale CRISPR-Cas9 Knockout

Yang

Kuang

et al. 2020

Stem Cells International

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“…The successful utilization of CRISPR/Cas9-directed genome editing in plant species has been reported and also includes the two relatives, i.e., the legume models G. max and M. truncatula (Li et al, 2015 ; Meng et al, 2017 ). CRISPR/Cas9 gene editing technology is currently revolutionizing genetic studies and crop improvement because it can be applied with high-throughput and at genome-scale (Yang et al, 2017 ). To our knowledge, no research effort has been made to implement this system in chickpea.…”

Section: Perspectivesmentioning

confidence: 99%

Viruses and Phytoparasitic Nematodes of Cicer arietinum L.: Biotechnological Approaches in Interaction Studies and for Sustainable Control

et al. 2018

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Cicer arietinum L. (chickpea) is the world's fourth most widely grown pulse. Chickpea seeds are a primary source of dietary protein for humans, and chickpea cultivation contributes to biological nitrogen fixation in the soil, given its symbiotic relationship with rhizobia. Therefore, chickpea cultivation plays a pivotal role in innovative sustainable models of agro-ecosystems inserted in crop rotation in arid and semi-arid environments for soil improvement and the reduction of chemical inputs. Indeed, the arid and semi-arid tropical zones of Africa and Asia have been primary areas of cultivation and diversification. Yet, nowadays, chickpea is gaining prominence in Canada, Australia, and South America where it constitutes a main ingredient in vegetarian and vegan diets. Viruses and plant parasitic nematodes (PPNs) have been considered to be of minor and local impact in primary areas of cultivation. However, the introduction of chickpea in new environments exposes the crop to these biotic stresses, compromising its yields. The adoption of high-throughput genomic technologies, including genome and transcriptome sequencing projects by the chickpea research community, has provided major insights into genome evolution as well as genomic architecture and domestication. This review summarizes the major viruses and PPNs that affect chickpea cultivation worldwide. We also present an overview of the current state of chickpea genomics. Accordingly, we explore the opportunities that genomics, post-genomics and novel editing biotechnologies are offering in order to understand chickpea diseases and stress tolerance and to design innovative control strategies.

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“…These innovations have increased the upgrading of the CRISPR-Cas9 system as a huge and resourceful tool for genetic engineering [1]. CRISPR /Cas9 system has widely used in the editing of the genome in zebra fish, plants, rats, and human cell lines [10][11][12][13][14]. The number of investigations into the generation of mutants by CRISPR/CAS9 has risen significantly in modern years, especially for generally changed screening, due to the rapidly rising predominance of genome editing in biological research [2].…”

Section: Introduction Crisper/cas9 In Reviewmentioning

confidence: 99%

Article Review: Editing genome of Toxoplasma gondii by CRISPR/Cas9 system

Al-Ardi¹

2022

JUAPS

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Revolutionize Genetic Studies and Crop Improvement with High-Throughput and Genome-Scale CRISPR/Cas9 Gene Editing Technology

Cited by 22 publications

References 11 publications

Screening Genes Promoting Exit from Naive Pluripotency Based on Genome-Scale CRISPR-Cas9 Knockout

Screening Genes Promoting Exit from Naive Pluripotency Based on Genome-Scale CRISPR-Cas9 Knockout

Viruses and Phytoparasitic Nematodes of Cicer arietinum L.: Biotechnological Approaches in Interaction Studies and for Sustainable Control

Article Review: Editing genome of Toxoplasma gondii by CRISPR/Cas9 system

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