Revolutionary advances in 2‐methacryloyloxyethyl phosphorylcholine polymers as biomaterials

Ishihara, Kazuhíko

doi:10.1002/jbm.a.36635

Cited by 177 publications

(178 citation statements)

References 123 publications

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“…Excellent agreement in micelle size and low polydispersity between both methods were reported, again with a consistent hydrodynamic size difference where PMPC-PVBTMA was the largest and PEG-PLK was the smallest. The zPCMs exhibited a slightly larger hydrodynamic volume relative to the PCM counterparts, which is attributed to water solvating phosphorylcholine groups in the corona shell [26]. Figure 5 shows DLS and SAXS results for each neutral 10k -charged 100 system in 100 mM NaCl.…”

Section: Polyelectrolyte Complex Micelle (Pcm) and Zwitterionic Pcm (mentioning

confidence: 99%

Comparing Zwitterionic and PEG Exteriors of Polyelectrolyte Complex Micelles

et al. 2020

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A series of model polyelectrolyte complex micelles (PCMs) was prepared to investigate the consequences of neutral and zwitterionic chemistries and distinct charged cores on the size and stability of nanocarriers. Using aqueous reversible addition-fragmentation chain transfer (RAFT) polymerization, we synthesized a well-defined diblock polyelectrolyte system, poly(2-methacryloyloxyethyl phosphorylcholine methacrylate)-block-poly((vinylbenzyl) trimethylammonium) (PMPC-PVBTMA), at various neutral and charged block lengths to compare directly against PCM structure–property relationships centered on poly(ethylene glycol)-block-poly((vinylbenzyl) trimethylammonium) (PEG-PVBTMA) and poly(ethylene glycol)-block-poly(l-lysine) (PEG-PLK). After complexation with a common polyanion, poly(sodium acrylate), the resulting PCMs were characterized by dynamic light scattering (DLS) and small angle X-ray scattering (SAXS). We observed uniform assemblies of spherical micelles with a diameter ~1.5–2× larger when PMPC-PVBTMA was used compared to PEG-PLK and PEG-PVBTMA via SAXS and DLS. In addition, PEG-PLK PCMs proved most resistant to dissolution by both monovalent and divalent salt, followed by PEG-PVBTMA then PMPC-PVBTMA. All micelle systems were serum stable in 100% fetal bovine serum over the course of 8 h by time-resolved DLS, demonstrating minimal interactions with serum proteins and potential as in vivo drug delivery vehicles. This thorough study of the synthesis, assembly, and characterization of zwitterionic polymers in PCMs advances the design space for charge-driven micelle assemblies.

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Section: Polyelectrolyte Complex Micelle (Pcm) and Zwitterionic Pcm (mentioning

confidence: 99%

Comparing Zwitterionic and PEG Exteriors of Polyelectrolyte Complex Micelles

et al. 2020

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“…Among various amphiphilic polymers, MPC-based polymers are considered to be optimal due to their high cytocompatibility and high water solubility. [129][130][131] The polymers are composed of an MPC unit and a redox unit, and the EET property is expected to be controlled by various parameters, such as molecular weight, unit composition ratio, and the kind of redox unit (Figure 7a). Actually, redox-active phospholipid polymers, poly(MPC-co-VFc) (pMFc, E = +0.5 V, Figure 7b) [132] and Me 8 -pMFc (E = +0.18 V, Figure 7b) [133] with redox-active ferrocene analogues have been developed.…”

Section: Actual Cases Using Type II Mediatorsmentioning

confidence: 99%

Redox‐Active Polymers Connecting Living Microbial Cells to an Extracellular Electrical Circuit

Kaneko

Ishihara

Nakanishi

2020

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Microbial electrochemical systems in which metabolic electrons in living microbes have been extracted to or injected from an extracellular electrical circuit have attracted considerable attention as environmentally‐friendly energy conversion systems. Since general microbes cannot exchange electrons with extracellular solids, electron mediators are needed to connect living cells to an extracellular electrode. Although hydrophobic small molecules that can penetrate cell membranes are commonly used as electron mediators, they cannot be dissolved at high concentrations in aqueous media. The use of hydrophobic mediators in combination with small hydrophilic redox molecules can substantially increase the efficiency of the extracellular electron transfer process, but this method has side effects, in some cases, such as cytotoxicity and environmental pollution. In this Review, recently‐developed redox‐active polymers are highlighted as a new type of electron mediator that has less cytotoxicity than many conventional electron mediators. Owing to the design flexibility of polymer structures, important parameters that affect electron transport properties, such as redox potential, the balance of hydrophobicity and hydrophilicity, and electron conductivity, can be systematically regulated.

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“…Then, 0.5 mL of sodium dodecyl sulfate (SDS) solution (2 wt%) was added (30 min, 37 °C) to remove adsorbed proteins from the films. BCA Protein Assay kits (Thermo Fisher Scientific) were used to quantitatively assay the amount of eluted protein in the SDS solution at 578 nm 7b…”

Section: Methodsmentioning

confidence: 99%

“…In most cases, the nonfouling surfaces can also inhibit cell adhesion as a result of suppressed protein adsorption, which can improve the overall biocompatibility of long‐term implantable biomaterials . However, this is not ideal for tissue engineering as it needs cell adhesion and proliferation.…”

Section: Introductionmentioning

confidence: 99%

Zwitterionic PMCP‐Modified Polycaprolactone Surface for Tissue Engineering: Antifouling, Cell Adhesion Promotion, and Osteogenic Differentiation Properties

Chen

Lin

Feng

et al. 2019

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Biodegradable polycaprolactone (PCL) has been widely applied as a scaffold material in tissue engineering. However, the PCL surface is hydrophobic and adsorbs nonspecific proteins. Some traditional antifouling modifications using hydrophilic moieties have been successful but inhibit cell adhesion, which is not ideal for tissue engineering. The PCL surface is modified with bioinspired zwitterionic poly[2‐(methacryloyloxy)ethyl choline phosphate] (PMCP) via surface‐initiated atom transfer radical polymerization to improve cell adhesion through the unique interaction between choline phosphate (CP, on PMCP) and phosphate choline (PC, on cell membranes). The hydrophilicity of the PCL surface is significantly enhanced after surface modification. The PCL‐PMCP surface reduces nonspecific protein adsorption (e.g., up to 91.7% for bovine serum albumin) due to the zwitterionic property of PMCP. The adhesion and proliferation of bone marrow mesenchymal stem cells on the modified surface is remarkably improved, and osteogenic differentiation signs are detected, even without adding any osteogenesis‐inducing supplements. Moreover, the PCL‐PMCP films are more stable at the early stage of degradation. Therefore, the PMCP‐functionalized PCL surface promotes cell adhesion and osteogenic differentiation, with an antifouling background, and exhibits great potential in tissue engineering.

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Revolutionary advances in 2‐methacryloyloxyethyl phosphorylcholine polymers as biomaterials

Cited by 177 publications

References 123 publications

Comparing Zwitterionic and PEG Exteriors of Polyelectrolyte Complex Micelles

Comparing Zwitterionic and PEG Exteriors of Polyelectrolyte Complex Micelles

Redox‐Active Polymers Connecting Living Microbial Cells to an Extracellular Electrical Circuit

Zwitterionic PMCP‐Modified Polycaprolactone Surface for Tissue Engineering: Antifouling, Cell Adhesion Promotion, and Osteogenic Differentiation Properties

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