Revisiting the validity of the mouse tail suspension test: Systematic review and meta-analysis of the effects of prototypic antidepressants

Stukalin, Yelena; Lan, Anat; Einat, Haim

doi:10.1016/j.neubiorev.2020.01.034

Cited by 34 publications

(24 citation statements)

References 94 publications

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“…To analyze depression-like behavior in CF mice the tail suspension method was employed 29 . Mice were suspended by their tail for six minutes while being recorded.…”

Section: Resultsmentioning

confidence: 99%

Alleviation of depression-like behavior in a cystic fibrosis mouse model by Hdac6 depletion

Corey

Rymut

Kelley

2020

Sci Rep

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Cystic fibrosis (CF) patients experience heightened levels of anxiety and depression. Stress from dealing with chronic disease and rigorous treatment regimens certainly are primary contributors to these outcomes. We previously have demonstrated that microtubule alterations in CF are linked to a number of CF phenotypes including growth regulation and inflammatory responses to airway bacterial challenge. Deletion of histone deactelyase 6 (HDAC6), a cytosolic deacetylase that regulates tubulin acetylation, in CF mice restores growth and inflammatory phenotypes to wild type (WT) profiles. In this study, the hypothesis that Hdac6 depletion in CF mice would impact behaviors since Hda6 inhibition has been previously reported to have anti-depressive properties. Data demonstrate that CF mice exhibit reduced activity and reduced open arm time in an elevated plus maze test which can be consistent with anxiety-like behavior. CF mice also exhibit depression-like behaviors compared to WT mice in an age dependent manner. By eight weeks of age, CF mice exhibit significantly more immobile time in the tail-suspension test, however, Hdac6 depletion reverses the depressive phenotype. These data demonstrate that loss of CFTR function may predispose patients to experience depression and that this behavior is Hdac6 dependent.

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“…To analyze depression-like behavior in CF mice the tail suspension method was employed 29 . Mice were suspended by their tail for six minutes while being recorded.…”

Section: Resultsmentioning

confidence: 99%

Alleviation of depression-like behavior in a cystic fibrosis mouse model by Hdac6 depletion

Corey

Rymut

Kelley

2020

Sci Rep

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“…Yet, no correlation between these behaviours in the two tests was found (Table 2). Interestingly, two recent meta-analysis studies, one regarding the FST (Kara et al, 2018b) and the other regarding the TST (Stukalin et al, 2020), may indicate that these tests are very different. The FST was found to have validity in qualitatively predicting antidepressant-like effects, whereas the TST was found to also quantitatively show effects of dose across different experiments.…”

Section: Discussionmentioning

confidence: 99%

“…Water in the cylinders was replaced every three trials. (7) TSTthe TST is a standard test used for screening antidepressant activity (Steru et al, 1985;Kara et al, 2014) and was recently demonstrated to have relatively strong external validity (Stukalin et al, 2020). Mice were suspended 50 cm above the floor with an adhesive tape placed approximately 1 cm from the tip of the tail for a 6-min session.…”

Section: Behavioural Testsmentioning

confidence: 99%

Exploring test batteries for depression- and anxiety-like behaviours in female and male ICR and black Swiss mice

Kazavchinsky

Dahan

Einat

2020

Acta Neuropsychiatr.

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Objective and rationale: Animal models are critical for the study of mental disorders and their treatments but are repeatedly criticized for problems with validity and reproducibility. One approach to enhance validity and reproducibility of models is to use test batteries rather than single tests. Yet, a question regarding batteries is whether one can expect a consistent individual behavioural phenotype in mice across tests that can be presumed to be part of the same construct. This study was designed to explore the relationship between the behaviours of mice across tests in some variations of test batteries for depression- and anxiety-like behaviours. Methods: Female and male healthy, intact, and untreated mice from the ICR and black Swiss strains were used in four separate experiments. With some variations, mice were exposed to a battery of behavioural tests representing affective- and anxiety-like behaviours. Data were analysed for differences between sexes and for correlations between behaviours within and across the tests in the battery. Results: No differences were found between the sexes. With very few exceptions, we found correlations within tests (when one test has more than one measure or is repeated) but not across different tests within the battery. Conclusions: The results cast some doubt on the utility of behavioural test batteries to represent different facets of emotional behaviour in healthy intact outbred mice, without any interventions or treatments. Additional studies are designed to explore whether stronger relationship between the tests will appear after manipulations or drug treatments.

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“…The animals were carried on the open‐field test (OFT) (Lei et al, 2020), tail suspension test (TST) (Stukalin & Einat, 2020), forced swimming test (FST) (Campos et al, 2016) as the previous study described.…”

Section: Methodsmentioning

confidence: 99%

Schisandra chinensis ameliorates depressive‐like behaviors by regulating microbiota‐gut‐brain axis via its anti‐inflammation activity

Yan

Wang

Liu

et al. 2020

Phytotherapy Research

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The present study aimed to examine the antidepressant actions of the composition fractions of Schisandra chinensis using LPS‐induced mice. Animals were treated with total extracts (SCE), lignans (SCL), polysaccharides (SCPS), and essential oil (SCVO), and then subjected to behavioral tests. The forced swimming test (FST) and tail suspension test (TST) were used as predictive animal models of antidepressant activity. Total extracts and lignans significantly decreased the duration of immobility in FST and TST. We found that treatment with SCE and SCL could significantly decrease the levels of pro‐inflammatory cytokines both in the periphery and central nervous system (CNS). This was confirmed by the histopathological examination of the colon. The RT‐PCR results demonstrated that SCE and SCL could greatly inhibit the TLR4/NF‐κB/IKKα signaling pathway. In addition, the concentrations of Butyric acid and Propionic acid were upregulated by the administration, and the decreased diversity of intestinal microbiota and alterations of the relative proportions of Bacteroidetes and Firmicutes phylum members, Barnesiella and Lactobacillus genus members in LPS‐induced mice were restored as well. All results suggested that lignans is the effective fraction of S.chinensis to ameliorating depressive disorders, which its anti‐inflammation activity possibly involved in the bidirectional connection between gut microbiota and brain.

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Revisiting the validity of the mouse tail suspension test: Systematic review and meta-analysis of the effects of prototypic antidepressants

Cited by 34 publications

References 94 publications

Alleviation of depression-like behavior in a cystic fibrosis mouse model by Hdac6 depletion

Alleviation of depression-like behavior in a cystic fibrosis mouse model by Hdac6 depletion

Exploring test batteries for depression- and anxiety-like behaviours in female and male ICR and black Swiss mice

Schisandra chinensis ameliorates depressive‐like behaviors by regulating microbiota‐gut‐brain axis via its anti‐inflammation activity

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