Revisiting the systemic lipopolysaccharide mediated neuroinflammation: Appraising the effect of l -cysteine mediated hydrogen sulphide on it

Abstract

The present research was ventured to examine the effect of l-cysteine on neuro-inflammation persuaded by peripheral lipopolysaccharides (LPS, 125 μg/kg, i.p.) administration. No behavioral, biochemical, and inflammatory abnormality was perceived in the brain tissues of experimental animals after LPS administration. l-cysteine precipitated marginal symptoms of toxicity in the brain tissue. Similar pattern of wholesome effect of LPS were perceived when evaluated through the brain tissue fatty acid profile, histopathologically and NF-ĸBP65 protein expression. LPS was unsuccessful to alter the levels of hydrogen sulphide (HS), cyclooxygenase (COX) and lipoxygenase (LOX) enzyme in brain tissue. LPS afforded significant peripheral toxicity, when figured out through inflammatory markers (COX, LOX), gaseous signaling molecules nitric oxide (NO), HS, liver toxicity (SGOT, SGPT), and inflammatory transcription factor (NF-ĸBP65) and l-cysteine also provided a momentous protection against the same as well. The study inculcated two major finding, firstly LPS (i.p.) cannot impart inflammatory changes to brain and secondly, l-cysteine can afford peripheral protection against deleterious effect of LPS (i.p.).

Repurposing Combination Therapy of Voacamine with Vincristine for Down Regulation of HIF-1α/FASN Co-Axis and PHD2 Activation in ER+ Mammary Neoplasia

“…Various studies have reported the role of ROS in development of cancer manifested by the increase in TBARs, PC and reduction in the activity of SOD, GSH and Catalase [40,41]. Same type of manifestations in the antioxidant markers were also noted in DMBA treated rats.…”

Repurposing Combination Therapy of Voacamine With Vincristine for Downregulation of Hypoxia-Inducible Factor-1α/Fatty Acid Synthase Co-axis and Prolyl Hydroxylase-2 Activation in ER+ Mammary Neoplasia

“…Therefore, the evaluation of antioxidant machinery in the mammary gland tissue of experimental animals is critical. Various studies have reported the role of ROS in cancer, manifested by an increase in TBARS and PC and a reduction in the activity of SOD, GSH, and catalase ( Tiwari et al, 2016 ; Al-Saeedan et al, 2018 ). The same type of antioxidant marker was also observed in the DMBA-treated rats.…”

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“…Therefore, the evaluation of antioxidant machinery in the mammary gland tissue of experimental animals is critical. Various studies have reported the role of ROS in cancer, manifested by an increase in TBARS and PC and a reduction in the activity of SOD, GSH, and catalase (Tiwari et al, 2016;Al-Saeedan et al, 2018). The same type of antioxidant marker was also observed in the DMBAtreated rats.…”