Revisiting the shikimate pathway and highlighting their enzyme inhibitors

Almeida, Aline Marengoni; Marchiosi, Rogério; Abrahão, Josielle; Constantin, Rodrigo Polimeni; dos Santos, Wanderley Dantas; Ferrarese-Filho, Osvaldo

doi:10.1007/s11101-023-09889-6

Cited by 4 publications

(2 citation statements)

References 194 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The physiological significance of the different behaviors of these amino acids is currently unknown, but one scenario is that changes in their accumulation ratios may reflect their differential requirements as precursors for downstream metabolisms. AAAs serve as precursors for not only protein synthesis but also numerous plant-specialized metabolites in plants (Maeda & Dudareva, 2012; Yokoyama et al, 2021; Almeida et al, 2023). In watermelon, large numbers of alkaloids, shikimates, phenylpropanoids, and polyphenols are detected (Sorokina et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Influence of Monosporascus root rot and vine decline disease on the amino acid metabolism of watermelon fruit

Santo,

Tadano,

Inokami

et al. 2024

Preprint

View full text Add to dashboard Cite

Monosporascus root rot and vine decline (MRRVD) is a severe disease of watermelon, melon, and other cucurbits, characterized by root necrosis and sudden wilting at the later stage of fruit maturation. The present study examined the effects of MRRVD on the metabolism in watermelon fruits. The MRRVD-affected watermelon fruits had significantly lower lycopene and total solid contents, but polyphenols content and total antioxidant activities were comparable with the controls, suggesting that MRRVD inhibited the ripening processes but maintained defensive processes in the fruits. The MRRVD fruits showed a lower level of calcium than the controls, while the contents of other major nutrition minerals were not significantly altered. The MRRVD fruits had a lower content of total amino acids, and their composition was characterized by an increase in the percentage fractions for several amino acids including citrulline, which may reflect the physiological response to the MRRVD-related water deficit condition.

show abstract

Section: Discussionmentioning

confidence: 99%

Influence of Monosporascus root rot and vine decline disease on the amino acid metabolism of watermelon fruit

Santo,

Tadano,

Inokami

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…As a product of the shikimate pathway, chorismate is a natural branch point for various metabolic pathways [34][35][36]. It is abundant in many different cells including bacteria, archaea, fungi, and plants [37].…”

Section: Discussionmentioning

confidence: 99%

Engineering the C3N Pathway as a Short Detour for De Novo NAD+ Biosynthesis in Saccharomyces cerevisiae

Li,

Tang,

Ding

et al. 2023

Fermentation

View full text Add to dashboard Cite

As a life-essential coenzyme, nicotinamide adenine dinucleotide (NAD+) has been explored for more than a century. In Saccharomyces, the natural NAD+de novo biosynthetic pathway initiating from tryptophan has been well elucidated. To bypass this stringently controlled natural pathway in yeast, an economical C3N pathway that was developed in Escherichia coli previously was constructed in Saccharomyces as a short detour for de novo NAD+ biosynthesis. After the functional expressions of the C3N genes were identified in Saccharomyces cerevisiae BY4741 by in vitro enzymatic assays, the C3N module was introduced into an NAD+ auxotrophic S. cerevisiae strain BY01, in which the BNA2 gene encoding tryptophan 2,3-dioxygenase was inactivated. The efficient NAD+ synthesis via the C3N pathway was confirmed by both plate assays and fermentation analysis. The applicability of the C3N pathway in cofactor engineering was tested by introducing it into S. cerevisiae BY4741, which improved the cellular NAD(H) level considerably. Consequently, this study proved that the de novo NAD+ biosynthetic pathway can be replaced by an artificial pathway in yeast, which paves a way to design more promising schemes in eukaryotes for rational manipulation of the cellular NAD(H) levels.

show abstract