Revisiting the Role of the Myofibroblast in Socket Surgery: An Immunohistochemical Study

Tawfik, Hatem A.; Abdulhafez, Mohamed H.; Fouad, Yousef A; Rashed, Hazem; Osman, Wesam M.

doi:10.1097/iop.0000000000000510

Cited by 14 publications

(18 citation statements)

References 18 publications

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“…Tawfik et al have demonstrated histologically in an animal model that a single injection of mitomycin-C, triamcinolone, 5-Fluorouracil, or bevacizumab could reduce the number of myofibroblasts in an actively healing socket, reducing the tendency to socket contraction. 88 …”

Section: Contracted Socketmentioning

confidence: 99%

Anophthalmic Socket Syndrome: Prevalence, Impact and Management Strategies

Quaranta‐Leoni¹,

Fiorino²,

Quaranta‐Leoni³

et al. 2021

OPTH

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Anophthalmic socket syndrome determines functional deficits and facial deformities, and may lead to poor psychological outcomes. This review aims to comprehensively evaluate the features of the syndrome, based on literature review and authors’ clinical and surgical experience. An electronic database (PubMed,MEDLINE and Google Scholar) search of all articles written in English and non-English language with abstract translated to English on anophthalmic socket syndrome was performed. Data reviewed included demographics, presentations, investigations, management, complications and outcomes. Different types of orbital implants were evaluated; the management of implant exposure was examined; different orbital volume enhancement procedures such as secondary implantation, subperiosteal implants and the use of fillers in anophthalmic patients were described; the problems related to socket contraction were outlined; the treatment options for chronic anophthalmic socket pain and phantom eye syndrome were assessed; the most recent advances in the management of congenital anophthalmia were described. Current clinical evidence does not support a specific orbital implant; late exposure of porous implants may be due to pegging, which currently is seldom used; filler absorption in the orbit appears to be faster than in the dermis, and repeated treatments could be a potential source of inflammation; socket contraction results in significant functional and psychological disability, and management is challenging. Patients affected by anophthalmic socket pain and phantom eye syndrome need specific counseling. It is auspicable to use a standardized protocol to treat children affected by clinical congenital anophthalmia; dermis fat graft is a suitable option in these patients as it helps continued socket expansion. Dermis fat graft can also address the volume deficit in case of explantation of exposed implants and in contracted sockets in both children and adults. Appropriate clinical care is essential, as adequate prosthesis wearing improves the quality of life of anophthalmic patients.

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Section: Contracted Socketmentioning

confidence: 99%

Anophthalmic Socket Syndrome: Prevalence, Impact and Management Strategies

Quaranta‐Leoni¹,

Fiorino²,

Quaranta‐Leoni³

et al. 2021

OPTH

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“…Fibroblasts appear on day 2-3 following injury, while myofibroblasts appear on day 12 when wound contractions are nearly 80% complete [11,12].…”

Section: Introductionmentioning

confidence: 99%

“…TGF-β1 is a key mediator that induce α-SMA [11,12]. Alpha-smooth muscle actin (α-SMA) may serve as a reliable marker for myofibroblast cells [12], although others considered it as not a consistent marker for contractile fibroblasts and lots of collagen [13]. Alpha-SMA widely presents in stressed fibers induces cytoskeleton reorganization, increases contractility and stimulates cell migration.…”

Section: Introductionmentioning

confidence: 99%

“…TGF-β1 is a major profibrotic cytokine and increased expression plays an important role in the deposition of collagen and extracellular matrix, wound healing and scar formation in the socket contracture tissue [6]. TGF-β1 is a key mediator that induce α-SMA [11,12]. Alpha-smooth muscle actin (α-SMA) may serve as a reliable marker for myofibroblast cells [12], although others considered it as not a consistent marker for contractile fibroblasts and lots of collagen [13].…”

Section: Introductionmentioning

confidence: 99%

“…Studies on fibrosis were mostly performed using tissues other than socket contracture. Among few studies on socket contracture, one has demonstrated that myofibroblasts play an important role in modulating wound healing, tissue remodeling and organ deformation [12]. TGF-β1 is a major profibrotic cytokine and increased expression plays an important role in the deposition of collagen and extracellular matrix, wound healing and scar formation in the socket contracture tissue [6].…”

Section: Introductionmentioning

confidence: 99%

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α-SMA Expression Increased Over Cell Passages and Decreased by Exogenous TGF-β1, In Vitro Studies on Myofibroblast Derived from Orbital Socket Contracture

Dewi

Chairinnisa²,

Sujuti³

et al. 2018

J.Trop.Life.Science

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ABSTRACTα-smooth muscle actin (α-SMA), a marker of myofibroblast, induces cytoskeleton reorganization, increases contractility and stimulates cell migration in TGF-β1 induced stress fibers. The aims of the present study were to determine the level of α-SMA expression and morphological cell changes in different passages of myofibroblasts with varied TGF-β1 concentrations. Myofibroblast cell cultures were derived from fibrotic tissues of fourth degree socket contracture. The α-SMA expression level was measured in myofibroblast cultures passage I, II, and III with and without 10 ng/mL TGF-β1, and in passage III with 2.5; 5; 10; and 20 ng/mL TGF-β1. Results: The levels of α-SMA expression level in passage I to III were I 31.42 ± 3.4; 40.34 ± 8.14 and 56.37 ± 7.57, respectively. Addition of 10 ng/mL TGF-β1 into passage I-III myofibroblast cultures resulted in α-SMA expression level of 31.24 ± 2.93; 36.81 ± 6.09; and 14.29 ± 2.72, respectively. Myoblasts passage III showed the lowest α-SMA expression level following exposure to TGF-β1 10 ng/mL (22.37 ± 12.86) and highest without TGF-β1 (48.34 ± 13.36), however no morphological changes detected. α-SMA expression level increased with cell passages, decreases with addition of TGF-β1 while not affecting morphology of myofibroblast derived from the orbital socket contracture.

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Management of the Contracted Socket

Sales-Sanz,

Hartong

2024

Oculoplastic, Lacrimal and Orbital Surgery

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Revisiting the Role of the Myofibroblast in Socket Surgery: An Immunohistochemical Study

Cited by 14 publications

References 18 publications

Anophthalmic Socket Syndrome: Prevalence, Impact and Management Strategies

Anophthalmic Socket Syndrome: Prevalence, Impact and Management Strategies

α-SMA Expression Increased Over Cell Passages and Decreased by Exogenous TGF-β1, In Vitro Studies on Myofibroblast Derived from Orbital Socket Contracture

Management of the Contracted Socket

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