Revisiting the Metal-Binding Chemistry of Nicotianamine and 2′-Deoxymugineic Acid. Implications for Iron Nutrition in Strategy II Plants

Reichman, Suzie M.; Parker, David R.

doi:10.1104/pp.005009

Cited by 34 publications

(38 citation statements)

References 17 publications

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“…Thus nicotianamine plays a crucial role in the movement of transition metal ions across various membrane barriers, such as the loading of phloem in the leaf [2,7,8,21,[23][24][25][26]48]. It is also proposed that complexation of iron by NA suppresses oxidative damage from Fenton chemistry associated with redox cycling between Fe 3+ and Fe 2+ [41,49].…”

Section: Introductionmentioning

confidence: 96%

A speciation model of essential trace metal ions in phloem

Harris

Sammons

Grabiak

2012

Journal of Inorganic Biochemistry

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Section: Introductionmentioning

confidence: 96%

A speciation model of essential trace metal ions in phloem

Harris

Sammons

Grabiak

2012

Journal of Inorganic Biochemistry

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“…Especially, NA has been investigated extensively in the last years as a candidate ligand for transport of iron and other metals, but the mechanistic details of respective processes are still under discussion. In particular, the importance and respective stabilities of ferrous and ferric NA species in planta is not completely clear [13][14][15] …”

mentioning

confidence: 99%

CE of phytosiderophores and related metal species in plants

et al. 2007

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Phytosiderophores (PS) and the closely related substance nicotianamine (NA) are key substances in metal uptake into graminaceous plants. Here, the CE separation of these substances and related metal species is demonstrated. In particular, the three PS 2'-deoxymugineic acid (DMA), mugineic acid (MA), and 3-epi-hydroxymugineic acid (epi-HMA), and NA, are separated using MES/Tris buffer at pH 7.3. Moreover, three Fe(III) species of the different PS are separated without any stability problems, which are often present in chromatographic analyses. Also divalent metal species of Cu, Ni, and Zn with the ligands DMA and NA are separated with the same method. By using a special, zwitterionic CE capillary, even the separation of two isomeric Fe(III) chelates with the ligand ethylenediamine-N,N'-bis(o-hydroxyphenyl)acetic acid (EDDHA) is possible (i.e., meso-Fe(III)-EDDHA and rac-Fe(III)-EDDHA), and for fast separations of NA and respective divalent and trivalent metal species, a polymer CE microchip with suppressed EOF is described. The proposed CE method is applicable to real plant samples, and enables to detect changes of metal species (Cu-DMA, Ni-NA), which are directly correlated to biological processes.

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“…Recent reports uncovered that inside the vacuole, NA functions in Zn chelation (Haydon et al, 2012) and that the complex Zn-NA occurs in the phloem sap (Nishiyama et al, 2012). Inside the phloem, which has neutral pH values, the stability of the Fe-NA complexes seems optimal, and it was proposed that inside the phloem sap NA might chelate Fe (von Wiren et al, 1999;Reichman and Parker, 2002;Weber et al, 2006;Rellán-Alvarez et al, 2008). NA prevents Fe precipitation and promotes the delivery of Fe to its target sites (Becker et al, 1995), and in addition, it minimizes Fe toxicity as the chelation prevents radical production via the Fenton reaction .…”

Section: Introductionmentioning

confidence: 99%

“…One of the most effective strategies to combat human malnutrition is breeding major staple food crops enriched in bio-available micronutrients. The metal chelator and nonproteinogenic amino acid nicotianamine (NA) is known to bind a range of metal ions, including Fe 2+ , Fe 3+ , and Zn 2+ (Anderegg and Ripperger, 1989;Scholz et al, 1992;von Wiren et al, 1999;Reichman and Parker, 2002;Schmiedeberg et al, 2003;Weber et al, 2006;Rellán-Alvarez et al, 2008;Nishiyama et al, 2012). NA promotes high Fe content and Fe bioavailability in cereal grains (Lee et al, 2009;Zheng et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Nicotianamine Functions in the Phloem-Based Transport of Iron to Sink Organs, in Pollen Development and Pollen Tube Growth in Arabidopsis

et al. 2012

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The metal chelator nicotianamine promotes the bioavailability of Fe and reduces cellular Fe toxicity. For breeding Fe-efficient crops, we need to explore the fundamental impact of nicotianamine on plant development and physiology. The quadruple nas4x-2 mutant of Arabidopsis thaliana cannot synthesize any nicotianamine, shows strong leaf chlorosis, and is sterile. To date, these phenotypes have not been fully explained. Here, we show that sink organs of this mutant were Fe deficient, while aged leaves were Fe sufficient. Upper organs were also Zn deficient. We demonstrate that transport of Fe to aged leaves relied on citrate, which partially complemented the loss of nicotianamine. In the absence of nicotianamine, Fe accumulated in the phloem. Our results show that rather than enabling the long-distance movement of Fe in the phloem (as is the case for Zn), nicotianamine facilitates the transport of Fe from the phloem to sink organs. We delimit nicotianamine function in plant reproductive biology and demonstrate that nicotianamine acts in pollen development in anthers and pollen tube passage in the carpels. Since Fe and Zn both enhance pollen germination, a lack of either metal may contribute to the reproductive defect. Our study sheds light on the physiological functions of nicotianamine.

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Revisiting the Metal-Binding Chemistry of Nicotianamine and 2′-Deoxymugineic Acid. Implications for Iron Nutrition in Strategy II Plants

Cited by 34 publications

References 17 publications

A speciation model of essential trace metal ions in phloem

A speciation model of essential trace metal ions in phloem

CE of phytosiderophores and related metal species in plants

Nicotianamine Functions in the Phloem-Based Transport of Iron to Sink Organs, in Pollen Development and Pollen Tube Growth in Arabidopsis

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