Revisiting poly(A)‐binding proteins: Multifaceted regulators during gametogenesis and early embryogenesis

Zhao, Long‐Wen; Fan, Heng‐Yu

doi:10.1002/bies.202000335

Cited by 14 publications

(10 citation statements)

References 120 publications

(215 reference statements)

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“…As multiple eIF4E family members in germ cells, PABPs also have distinct expression patterns. [ 51 ] These results suggest that it is possible to assume a potential oocyte‐specific closed‐loop‐model: eIF4E1B‐eIF4G‐PABPC1L, which can be used to control various target genes to promote the stability and translation efficiency of multiple developmental events during OET.…”

Section: Discussionmentioning

confidence: 99%

Selective Translation of Maternal mRNA by eIF4E1B Controls Oocyte to Embryo Transition

et al. 2023

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Maternal messenger ribonucleic acids (mRNAs) are driven by a highly orchestrated scheme of recruitment to polysomes and translational activation. However, selecting and regulating individual mRNAs for the translation from a competitive pool of mRNAs are little‐known processes. This research shows that the maternal eukaryotic translation initiation factor 4e1b (Eif4e1b) expresses during the oocyte‐to‐embryo transition (OET), and maternal deletion of Eif4e1b leads to multiple defects concerning oogenesis and embryonic developmental competence during OET. The linear amplification of complementary deoxyribonucleic acid (cDNA) ends, and sequencing (LACE‐seq) is used to identify the distinct subset of mRNA and its CG‐rich binding sites within the 5′ untranslated region (UTR) targeted by eIF4E1B. The proteomics analyses indicate that eIF4E1B‐specific bound genes show stronger downregulation at the protein level, which further verify a group of proteins that plays a crucial role in oocyte maturation and embryonic developmental competence is insufficiently synthesized in Eif4e1b‐cKO oocytes during OET. Moreover, the biochemical results in vitro are combined to further confirm the maternal‐specific translation activation model assembled by eIF4E1B and 3′UTR‐associated mRNA binding proteins. The findings demonstrate the indispensability of eIF4E1B for selective translation activation in mammalian oocytes and provide a potential network regulated by eIF4E1B in OET.

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Section: Discussionmentioning

confidence: 99%

Selective Translation of Maternal mRNA by eIF4E1B Controls Oocyte to Embryo Transition

et al. 2023

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“…Poly (A) binding protein (PABP) belongs to a class of highly conserved RNA‐binding proteins (RBPs). PABP is classified into two types: nuclear poly (A) binding protein (PABPN) and cytoplasmic poly (A) binding protein (PABPC) 7 . PABPC1 mediates messenger RNA (mRNA) translation, degradation, and cytoplasmic polyadenylation 8,9 .…”

Section: Introductionmentioning

confidence: 99%

PABPC1 promotes cell proliferation and metastasis in pancreatic adenocarcinoma by regulating COL12A1 expression

Yao

et al. 2023

Immunity Inflam & Disease

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Background: The expression of cytoplasmic poly (A) binding protein-1 (PABPC1) has been reported in multiple cancer types. This protein is known to modulate cancer progression. However, the effects of PABPC1 expression in pancreatic adenocarcinoma (PAAD) have not been investigated. Here, we investigate the regulatory targets and molecular mechanisms of PABPC1 in PAAD. Methods: PABPC1 and collagen type XII α1 chain (COL12A1) expression in PAAD and their role in tumor prognosis and tumor stage were investigated using The Cancer Genome Atlas database analysis. After silencing PABPC1, messenger RNA sequencing and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed. The expression of differentially expressed genes (DEGs), cell viability, apoptosis, and cell migration and invasion were explored using reverse transcriptionquantitative polymerase chain reaction, Cell Counting Kit-8 assay, flow cytometry assay, and transwell assay, respectively. The relationship between PABPC1 and COL12A1 expression was assessed by Pearson's correlation analysis. The regulatory function of COL12A1 in PABPC1-affected BXPC3 cell behavior was studied after COL12A1 was overexpressed.Results: PABPC1 and COL12A1 expression was upregulated in patients with PAAD and was linked to poor prognosis. Four hundred and seventy-four DEGs were observed in BXPC3 cells after PABPC1 silencing. GO and KEGG analyses revealed that the top 10 DEGs were enriched in cell adhesion pathways. Additionally, PABPC1 silencing inhibited cell viability, migration, and invasion and accelerated apoptosis in BXPC3 cells. PABPC1 silencing increased AZGP1 and ARHGAP30 expression and decreased CAV1 and

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“…The PABPs are grouped based on their subcellular localization: i) nuclear PABPs (PABPNs: PABPN1 and PABPN1L), and ii) cytoplasmic PABPs (PABPCs: PABPC1, PABPC3, PABPC4, PABPC4L, PABPC5, and ePAB) (19). The PABPs lack catalytic domains but instead function as scaffold proteins as they bind to poly(A) tails via their RNA recognition motifs (RRMs) (20). The best-known function of PABPN1 is to stimulate poly(A) polymerase (PAP)-dependent polyadenylation (21, 22).…”

Section: Introductionmentioning

confidence: 99%

ZC3H11A interacts with PABPN1 and alters polyadenylation of viral transcripts

Kases

Schubert

Hajikhezri

et al. 2023

Preprint

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Nuclear mRNA metabolism is regulated by multiple proteins, which either directly bind to RNA or form multi-protein complexes. The RNA-binding protein ZC3H11A is involved in nuclear mRNA export, and NF-kB signaling and is essential during mouse embryo development. Furthermore, previous studies have shown that ZC3H11A is important for nuclear-replicating viruses. However, detailed biochemical characterization of the ZC3H11A protein has been lacking. In this study, we established the ZC3H11A protein interactome in human and mouse cells. We demonstrate that the nuclear poly(A)-binding protein PABPN1 interacts specifically with the ZC3H11A protein and controls ZC3H11A localization into nuclear speckles. We report that ZC3H11A specifically interacts with the human adenovirus type 5 (HAdV-5) capsid mRNA in a PABPN1-dependent manner. Notably, ZC3H11A uses the same zinc finger motifs to interact with PABPN1 and mRNA, with a single zinc finger motif responsible for these functions. Further, we demonstrate that the lack of ZC3H11A alters the polyadenylation of HAdV-5 capsid mRNA. Taken together, our results suggest that the ZC3H11A protein acts as a novel regulator of polyadenylation of nuclear mRNA.

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Revisiting poly(A)‐binding proteins: Multifaceted regulators during gametogenesis and early embryogenesis

Cited by 14 publications

References 120 publications

Selective Translation of Maternal mRNA by eIF4E1B Controls Oocyte to Embryo Transition

Selective Translation of Maternal mRNA by eIF4E1B Controls Oocyte to Embryo Transition

PABPC1 promotes cell proliferation and metastasis in pancreatic adenocarcinoma by regulating COL12A1 expression

ZC3H11A interacts with PABPN1 and alters polyadenylation of viral transcripts

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