Revisiting germinal matrix and ventricular lining cells in cerebrospinal fluid: Potential mimickers of intracranial malignancy

Wysozan, Timothy R.; Gulati, Rohit

doi:10.1002/dc.24703

Cited by 2 publications

(2 citation statements)

References 9 publications

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“…Since morphologic review is often performed on cytospin preparations, distortion of cell morphology may make the distinction of blasts from other cell types such as reactive lymphocytes and germinal matrix cells challenging (Figure 2). [24][25][26] Factors such as time-in-transit, specimen preparation, and cytocentrifuge instrumentation/ rotor speed may impact the quality of cell preparations, and efforts to harmonise these systems may result in a more reliable product. 27 The use of albumin or other proteins to stabilise cell membranes has been recommended to improve cell preservation.…”

Section: Study Selectionmentioning

confidence: 99%

Technological features of blast identification in the cerebrospinal fluid: A systematic review of flow cytometry and laboratory haematology methods

Frater

Shirai

Brestoff

2022

Int J Lab Hematology

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Background: Involvement of the central nervous system (CNS) by acute leukemias (ALs) has important implications for risk stratification and disease outcome. The clinical laboratory plays an essential role in assessment of cerebrospinal fluid (CSF) specimens from patients with ALs at initial diagnosis, at the end of treatment, and when CNS involvement is clinically suspected. The two challenges for the laboratory are 1) to accurately provide a cell count of the CSF and 2) to successfully distinguish blasts from other cell types. These tasks are classically performed using manual techniques, which suffer from suboptimal turnaround time, imprecision, and inconsistent interoperator performance. Technological innovations in flow cytometry and hematology analyzer technology have provided useful complements and/or alternatives to conventional manual techniques.Aims: We performed a PRISMA-compliant systematic review to address the medical literature regarding the development and current state of the art of CSF blast identification using flow cytometry and laboratory hematology technologies. Materials and Methods:We searched the peer reviewed medical literature using MEDLINE (PubMed interface), Web of Science, and Embase using the keywords "CSF or cerebrospinal" AND "blasts(s)".Results: 108 articles were suitable for inclusion in our systematic review. These articles covered 1) clinical rationale for CSF blast identification; 2) morphology-based CSF blast identification; 3) the role of flow cytometry; 4) use of hematology analyzers for CSF blast identification; and 5) quality issues. 9 /L, which is much lower than the original machine count and platelet transfusion was warranted.Discussion: 1) Clinical laboratory testing plays a central role in risk stratification and clinical management of patients with acute leukemias, most clearly in pediatric ALs;2) studies focused on other patient populations, including adults and patients with AML are less prevalent in the literature; 3) improvements in instrumentation may provide better performance for the classification of CSF specimens. Conclusion:Current challenges include: 1) more precisely characterizing the natural history of AL involvement of the CNS, 2) improvements in automated cell count technology of low cellularity specimens, 3) defining the role of flow MRD testing of CSF specimens and 4) improved recognition of specimen quality by clinicians and laboratory personnel.

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Section: Study Selectionmentioning

confidence: 99%

Technological features of blast identification in the cerebrospinal fluid: A systematic review of flow cytometry and laboratory haematology methods

Frater

Shirai

Brestoff

2022

Int J Lab Hematology

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“…Post hemorrhagic components are frequently encountered on light microscopic evaluation of the cerebrospinal fluid (CSF), as early as 1-2 days after bleeding [59,60]. In addition to the erythrophagocytosis, cellular components of the ventricular lining (ependymal cells and choroid plexus cells) and rarely, precursor germinal matrix cells (due to close proximity with disrupted ventricular lining) may be identified in CSF analysis [61][62][63].…”

Section: Complications and Potential Treatment Strategiesmentioning

confidence: 99%

Germinal Matrix-Intraventricular Hemorrhage: Current Concepts and Future Direction

Sood¹,

Gulati²

2022

Cerebrospinal Fluid

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Germinal Matrix Hemorrhage -Intraventricular hemorrhage (IVH) is a bleed of multifactorial etiology involving the highly vascular and delicate neuro-glial precursors in the developing brain. It poses a challenging complication in preterm newborns. This chapter provides a focused discussion on the current concepts in pathogenesis, management, and complications of IVH. The radiological findings at diagnosis and follow-up and the cytological features of CSF will be valuable to both frontline and diagnostic healthcare providers. The chapter also reviews the ongoing scientific development in the field. The authors believe that this chapter will be a valuable tool for all healthcare providers (students, physicians, and in nursing care) in managing this challenging condition.

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Revisiting germinal matrix and ventricular lining cells in cerebrospinal fluid: Potential mimickers of intracranial malignancy

Cited by 2 publications

References 9 publications

Technological features of blast identification in the cerebrospinal fluid: A systematic review of flow cytometry and laboratory haematology methods

Technological features of blast identification in the cerebrospinal fluid: A systematic review of flow cytometry and laboratory haematology methods

Germinal Matrix-Intraventricular Hemorrhage: Current Concepts and Future Direction

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