Revisiting energy expenditure: how to correct mouse metabolic rate for body mass

Müller, Timo D.; Klingenspor, Martin; Tschöp, Matthias H.

doi:10.1038/s42255-021-00451-2

Cited by 75 publications

(59 citation statements)

References 10 publications

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“…Body mass was included and kept as covariate in all tested models. Changes in metabolic rate were analyzed with m b as a covariate because this approach allows discriminating between mass-dependent and mass-independent differences in metabolic rate between groups (Packard and Boardman 1988 ; Tschöp et al 2012 ; Fernández-Verdejo et al 2019 ; Müller et al 2021 ). Type of cage and nesting material were retained as fixed factors in final model of the first analysis, and the effect of housing in pairs was retained in final model of the second analysis.…”

Section: Methodsmentioning

confidence: 99%

Housing conditions modify seasonal changes in basal metabolism and body mass of the Siberian hamster, Phodopus sungorus

Jefimow

Przybylska-Piech

2022

J Comp Physiol B

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Proper housing conditions are important aspects of animal welfare. Animals housed in enriched environments show less stereotypic behaviours than animals kept in barren cages. However, different types of cage enrichment may affect the results of experimental studies and hinder comparative analyses of animal physiology and behaviour. We investigated whether access to a running wheel, availability of nesting material, and pair housing affect basal metabolic rate (BMR) of Siberian hamsters (Phodopus sungorus) under various acclimation conditions. We used 70 adult hamsters (35 males and 35 females) divided into five groups housed under different cage conditions. All individuals experienced the same acclimation procedure: first a winter (L8:D16) then a summer (L16:D8) photoperiod, at air temperatures of first 20 °C then 7 °C under both photoperiods. We found that nesting material and pair housing did not affect hamster BMR, while access to a running wheel increased BMR and body mass regardless of photoperiod and ambient temperature. Thus, we suggest that cage enrichment should be applied with caution, especially in studies on energetics or thermoregulation, particularly in seasonal animals.

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Section: Methodsmentioning

confidence: 99%

Housing conditions modify seasonal changes in basal metabolism and body mass of the Siberian hamster, Phodopus sungorus

Jefimow

Przybylska-Piech

2022

J Comp Physiol B

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“…1h for graphical illustration). Moreover, because the deviation of each data point from the regression line is preserved under ANCOVA 38 , it enables visualization of group differences at a given body weight. The results showed that the predicted EE based on a fixed body weight, arbitrarily set at 30 g, was reduced in old mice in response to both HIIT and MICT (Fig.…”

Section: Resultsmentioning

confidence: 99%

Age-dependent impact of two exercise training regimens on genomic and metabolic remodeling in skeletal muscle and liver of male mice

et al. 2022

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Skeletal muscle adapts to different exercise training modalities with age; however, the impact of both variables at the systemic and tissue levels is not fully understood. Here, adult and old C57BL/6 male mice were assigned to one of three groups: sedentary, daily high-intensity intermittent training (HIIT), or moderate intensity continuous training (MICT) for 4 weeks, compatible with the older group’s exercise capacity. Improvements in body composition, fasting blood glucose, and muscle strength were mostly observed in the MICT old group, while effects of HIIT training in adult and old animals was less clear. Skeletal muscle exhibited structural and functional adaptations to exercise training, as revealed by electron microscopy, OXPHOS assays, respirometry, and muscle protein biomarkers. Transcriptomics analysis of gastrocnemius muscle combined with liver and serum metabolomics unveiled an age-dependent metabolic remodeling in response to exercise training. These results support a tailored exercise prescription approach aimed at improving health and ameliorating age-associated loss of muscle strength and function in the elderly.

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“…When the latter indicated significant difference, individual group differences were computed using Bonferroni test. For energy expenditure, ANCOVA analysis was performed with body weight as a covariate [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

Suppression of obesity by melatonin through increasing energy expenditure and accelerating lipolysis in mice fed a high-fat diet

Li²,

Li³

et al. 2022

Nutr. Diabetes

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Backgrounds/objectives Melatonin promotes brown adipose tissue (BAT) activity, leading to body mass reduction and energy expenditure. However, the mechanisms governing these beneficial effects are not well-established. This study aimed to assess the effects of (1) melatonin on BAT and energy metabolism, and (2) fibroblast growth factor 21 (FGF21) in BAT-mediated thermogenesis. Methods Male C57BL/6 J mice received a high-fat diet (HFD) or normal chow, accompanied by intraperitoneal injection of 20 mg/kg melatonin for 12 weeks. FGF21−/− mice consumed an HFD with or without melatonin for 8 weeks. Results Melatonin attenuated weight gain, insulin resistance, adipocyte hypertrophy, inflammation, and hepatic steatosis induced by the HFD and increased energy expenditure. Furthermore, melatonin improved cold tolerance by increasing BAT uncoupling protein 1 (UCP1) expression and producing heat. Notably, melatonin resulted in a shift in energy metabolism favouring the utilization of fat, and it increased FGF21 in circulating and metabolic tissues and skeletal muscle phosphorylation of AMP-activated protein kinase. However, melatonin did not protect against obesity, insulin resistance, and energy expenditure in HFD-fed FGF21−/− mice. Conclusions Melatonin suppressed obesity and insulin resistance resulting from the HFD by enhancing BAT activity and energy expenditure, and these effects were dependent on FGF21.

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Revisiting energy expenditure: how to correct mouse metabolic rate for body mass

Cited by 75 publications

References 10 publications

Housing conditions modify seasonal changes in basal metabolism and body mass of the Siberian hamster, Phodopus sungorus

Housing conditions modify seasonal changes in basal metabolism and body mass of the Siberian hamster, Phodopus sungorus

Age-dependent impact of two exercise training regimens on genomic and metabolic remodeling in skeletal muscle and liver of male mice

Suppression of obesity by melatonin through increasing energy expenditure and accelerating lipolysis in mice fed a high-fat diet

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