Revisiting amino acid analyses for bioadhesives including a direct comparison of tick attachment cement (Dermacentor marginatus) and barnacle cement (Lepas anatifera)

Engel, Benedikt; Suppan, Johannes; Nürnberger, Sylvia; Power, Anne; Marchetti‐Deschmann, Martina

doi:10.1016/j.ijadhadh.2020.102798

Cited by 7 publications

(6 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5 ), I. scapularis tick cement proteins have high content of hydrophobic residues, followed by hydrophilic and basic amino acid residues. Data here is similar to chemical analyses findings of high content of hydrophobic residues in tick cement 3 , 4 , 54 . The amino acid residue composition in tick cement proteins is comparable to major extracellular matrix (ECM) in the skin.…”

Section: Resultssupporting

confidence: 90%

Identification and characterization of proteins that form the inner core Ixodes scapularis tick attachment cement layer

Mulenga

Radulović

Porter

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Ixodes scapularis long-term blood feeding behavior is facilitated by a tick secreted bio adhesive (tick cement) that attaches tick mouthparts to skin tissue and prevents the host from dislodging the attached tick. Understanding tick cement formation is highly sought after as its disruption will prevent tick feeding. This study describes proteins that form the inner core layer of I. scapularis tick cement as disrupting these proteins will likely stop formation of the outer cortical layer. The inner core cement layer completes formation by 24 h of tick attachment. Thus, we used laser-capture microdissection to isolate cement from cryosections of 6 h and 24 h tick attachment sites and to distinguish between early and late inner core cement proteins. LC–MS/MS analysis identified 138 tick cement proteins (TCPs) of which 37 and 35 were unique in cement of 6 and 24 h attached ticks respectively. We grouped TCPs in 14 functional categories: cuticular protein (16%), tick specific proteins of unknown function, cytoskeletal proteins, and enzymes (13% each), enzymes (10%), antioxidant, glycine rich, scaffolding, heat shock, histone, histamine binding, proteases and protease inhibitors, and miscellaneous (3–6% each). Gene ontology analysis confirm that TCPs are enriched for bio adhesive properties. Our data offer insights into tick cement bonding patterns and set the foundation for understanding the molecular basis of I. scapularis tick cement formation.

show abstract

Section: Resultssupporting

confidence: 90%

Identification and characterization of proteins that form the inner core Ixodes scapularis tick attachment cement layer

Mulenga

Radulović

Porter

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The area of the corresponding band component increases (1.33 to 9.21%) after exposure to SO 2 in combination with 80%RH. Considering that phenylalanine is known to be affected by acids [ 47 ], this increase in the band area was expected. It suggests that the oxidizing nature of SO 2 induces the formation of oxidation products, such as di-tryptophan and its multiple stereoisomers [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

Spectral Features Differentiate Aging-Induced Changes in Parchment—A Combined Approach of UV/VIS, µ-ATR/FTIR and µ-Raman Spectroscopy with Multivariate Data Analysis

et al. 2023

Self Cite

View full text Add to dashboard Cite

From the moment of production, artworks are constantly exposed to changing environmental factors potentially inducing degradation. Therefore, detailed knowledge of natural degradation phenomena is essential for proper damage assessment and preservation. With special focus on written cultural heritage, we present a study on the degradation of sheep parchment employing accelerated aging with light (295–3000 nm) for one month, 30/50/80% relative humidity (RH) and 50 ppm sulfur dioxide with 30/50/80%RH for one week. UV/VIS spectroscopy detected changes in the sample surface appearance, showing browning after light-aging and increased brightness after SO2-aging. Band deconvolution of ATR/FTIR and Raman spectra and factor analysis of mixed data (FAMD) revealed characteristic changes of the main parchment components. Spectral features for degradation-induced structural changes of collagen and lipids turned out to be different for the employed aging parameters. All aging conditions induced denaturation (of different degrees) indicated by changes in the secondary structure of collagen. Light treatment resulted in the most pronounced changes for collagen fibrils in addition to backbone cleavage and side chain oxidations. Additional increased disorder for lipids was observed. Despite shorter exposure times, SO2-aging led to a weakening of protein structures induced by transitions of stabilizing disulfide bonds and side chain oxidations.

show abstract

“…As omic sequencing continues to improve, both in the types of species that are sequenced and the quality of the sequencing data, this work provides a roadmap to reveal the fundamental biomolecular contributors to one of nature's most robust marine adhesives. Quantification of the amino acid composition of the entire adhesive for each species [ 91 ] and the abundance of each type of protein, as well as in vitro experiments examining species specific differences in fibril formation [ 18 , 19 ] or other traits are additional steps that will significantly contribute to understanding the role of bulk proteins in adhesion and whether these proteins have become more specialized across barnacle lineages. Finally, continued improvements in sample preparation, for both gDNA/mRNA and proteins, and the application of standard collection and sample processing methods to the adhesive from different species will enhance the ability for direct comparison of different species, highlighting both the similarities and interspecies differences in the adhesive proteome.…”

Section: Discussionmentioning

confidence: 99%

“…As omic sequencing continues to improve, both in the types of species that are sequenced and the quality of the sequencing data, this work provides a roadmap to reveal the fundamental biomolecular contributors to one of nature's most robust marine adhesives. Quantification of the amino acid composition of the entire adhesive for each species [91] and the abundance of each type of protein, as well as in vitro experiments examining species specific differences in fibril formation [18,19] or royalsocietypublishing.org/journal/rsob Open Biol. 11: 210142 other traits are additional steps that will significantly contribute to understanding the role of bulk proteins in adhesion and whether these proteins have become more specialized across barnacle lineages.…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of stalked and acorn barnacle adhesive proteomes

et al. 2021

View full text Add to dashboard Cite

Barnacles interest the scientific community for multiple reasons: their unique evolutionary trajectory, vast diversity and economic impact—as a harvested food source and also as one of the most prolific macroscopic hard biofouling organisms. A common, yet novel, trait among barnacles is adhesion, which has enabled a sessile adult existence and global colonization of the oceans. Barnacle adhesive is primarily composed of proteins, but knowledge of how the adhesive proteome varies across the tree of life is unknown due to a lack of genomic information. Here, we supplement previous mass spectrometry analyses of barnacle adhesive with recently sequenced genomes to compare the adhesive proteomes of Pollicipes pollicipes (Pedunculata) and Amphibalanus amphitrite (Sessilia). Although both species contain the same broad protein categories, we detail differences that exist between these species. The barnacle-unique cement proteins show the greatest difference between species, although these differences are diminished when amino acid composition and glycosylation potential are considered. By performing an in-depth comparison of the adhesive proteomes of these distantly related barnacle species, we show their similarities and provide a roadmap for future studies examining sequence-specific differences to identify the proteins responsible for functional differences across the barnacle tree of life.

show abstract

Revisiting amino acid analyses for bioadhesives including a direct comparison of tick attachment cement (Dermacentor marginatus) and barnacle cement (Lepas anatifera)

Cited by 7 publications

References 29 publications

Identification and characterization of proteins that form the inner core Ixodes scapularis tick attachment cement layer

Identification and characterization of proteins that form the inner core Ixodes scapularis tick attachment cement layer

Spectral Features Differentiate Aging-Induced Changes in Parchment—A Combined Approach of UV/VIS, µ-ATR/FTIR and µ-Raman Spectroscopy with Multivariate Data Analysis

Comparative analysis of stalked and acorn barnacle adhesive proteomes

Contact Info

Product

Resources

About