Revisited mycolic acid pattern of Mycobacterium confluentis using thin-layer chromatography

Secanella-Fandos, Silvia; Luquin, Marina; Pérez‐Trujillo, Míriam; Julián, Esther

doi:10.1016/j.jchromb.2011.08.001

Cited by 8 publications

(7 citation statements)

References 24 publications

(38 reference statements)

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“…Regarding phage typing, although sometimes useful (72,73), the limited number of mycobacteriophages identified and poor reproducibility make this method impractical for epidemiological studies (74,75). Phenotype-based typing methods, currently under intense investigation, analyze the composition of the mycobacterial cell wall by thin-layer chromatography (76)(77)(78), high-performance liquid chromatography (79,80), gas chromatography (81,82), and mass spectrometry (MS) (83)(84)(85). One promising methods is matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS.…”

Section: Phenotype-based Methodsmentioning

confidence: 99%

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

et al. 2016

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SUMMARYMolecular typing has revolutionized epidemiological studies of infectious diseases, including those of a mycobacterial etiology. With the advent of fingerprinting techniques, many traditional concepts regarding transmission, infectivity, or pathogenicity of mycobacterial bacilli have been revisited, and their conventional interpretations have been challenged. Since the mid-1990s, when the first typing methods were introduced, a plethora of other modalities have been proposed. So-called molecular epidemiology has become an essential subdiscipline of modern mycobacteriology. It serves as a resource for understanding the key issues in the epidemiology of tuberculosis and other mycobacterial diseases. Among these issues are disclosing sources of infection, quantifying recent transmission, identifying transmission links, discerning reinfection from relapse, tracking the geographic distribution and clonal expansion of specific strains, and exploring the genetic mechanisms underlying specific phenotypic traits, including virulence, organ tropism, transmissibility, or drug resistance. Since genotyping continues to unravel the biology of mycobacteria, it offers enormous promise in the fight against and prevention of the diseases caused by these pathogens. In this review, molecular typing methods forMycobacterium tuberculosisand nontuberculous mycobacteria elaborated over the last 2 decades are summarized. The relevance of these methods to the epidemiological investigation, diagnosis, evolution, and control of mycobacterial diseases is discussed.

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Section: Phenotype-based Methodsmentioning

confidence: 99%

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

et al. 2016

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“…For 2D TLC, a triple development with petroleum ether/acetone (95:5, v/v) in one direction was followed by a single development at 90˚C to the first direction with toluene/acetone (97:3, v/v), as described previously (24,36). The different spots of MA were designated a-MA, methoxy-MA, and keto-MA based on the functional groups, as reported previously (37). For purification of each class of MA, the crude MA was run on a preparative TLC plate, and spots were scraped off the plates and extracted with diethyl ether.…”

Section: Thin Layer Chromatographymentioning

confidence: 99%

“…1A). The total MA of both M. tuberculosis H37Rv and H37Ra contained a-MA, methoxy-MA, and keto-MA, whereas the total MA of M. smegmatis lacked keto-MA and methoxy-MA and that of M. phlei lacked methoxy-MA (18,37). Interestingly, a 10-14-fold transactivation of GAL4-TR4 was observed with the total MA from M. tuberculosis H37Rv/H37Ra and M. phlei; however, no significant transactivation was observed with the total MA from M. smegmatis (Fig.…”

Section: Statisticsmentioning

confidence: 99%

“…The separation and characterization of total MA from M. tuberculosis H37Rv was performed with 1D and 2D TLC as described (24,37), and the spots corresponding to a-MA, methoxy-MA, and keto-MA, were scraped and further extracted for analysis in the GAL4-based transactivation assay. The results of these assays confirm the involvement of keto-MA in the modulation of TR4 (Fig.…”

Section: Statisticsmentioning

confidence: 99%

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Mycobacterium tuberculosisKeto-Mycolic Acid and Macrophage Nuclear Receptor TR4 Modulate Foamy Biogenesis in Granulomas: A Case of a Heterologous and Noncanonical Ligand-Receptor Pair

Dkhar

Nanduri

Mahajan

et al. 2014

The Journal of Immunology

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The cell wall of Mycobacterium tuberculosis is configured of bioactive lipid classes that are essential for virulence and potentially involved in the formation of foamy macrophages (FMs) and granulomas. Our recent work established crosstalk between M. tuberculosis cell wall lipids and the host lipid-sensing nuclear receptor TR4. In this study, we have characterized, identified, and adopted a heterologous ligand keto-mycolic acid from among M. tuberculosis lipid repertoire for the host orphan NR TR4. Crosstalk between cell wall lipids and TR4 was analyzed by transactivation and promoter reporter assays. Mycolic acid (MA) was found to transactivate TR4 significantly compared with other cell wall lipids. Among the MA, the oxygenated form, keto-MA, was responsible for transactivation, and the identity was validated by TR4 binding assays followed by TLC and nuclear magnetic resonance. Isothermal titration calorimetry revealed that keto-MA binding to TR4 is energetically favorable. This keto-MA–TR4 axis seems to be essential to this oxygenated MA induction of FMs and granuloma formation as evaluated by in vitro and in vivo model of granuloma formation. TR4 binding with keto-MA features a unique association of host nuclear receptor with a bacterial lipid and adds to the presently known ligand repertoire beyond dietary lipids. Pharmacologic modulation of this heterologous axis may hold promise as an adjunct therapy to frontline tuberculosis drugs.

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“…Reversedephase high performance liquid chromatography (HPLC) is commonly used in many laboratories for the identification of the mycolic-acids patterns of mycobacteria [31]; however, this technology requires expertise with mycolic-acid patterns and unfortunately, structural types of mycolic acids cannot be identified. In contrast, using thin layer chromatography (TLC) is possible to identify the different structural types of mycolic acids contained in mycobacterial strains [32]. The MAC complex is characterized for the presence of mycolic acids I, IV and VI by TLC; however, the types of mycolic acid present in M. colombiense remain unknown.…”

Section: Introductionmentioning

confidence: 99%

Identification of potential biomarkers to distinguish Mycobacterium colombiense from other mycobacterial species

Leguizamon

Hernández

Murcia

et al. 2013

Molecular and Cellular Probes

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Revisited mycolic acid pattern of Mycobacterium confluentis using thin-layer chromatography

Cited by 8 publications

References 24 publications

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria

Mycobacterium tuberculosisKeto-Mycolic Acid and Macrophage Nuclear Receptor TR4 Modulate Foamy Biogenesis in Granulomas: A Case of a Heterologous and Noncanonical Ligand-Receptor Pair

Identification of potential biomarkers to distinguish Mycobacterium colombiense from other mycobacterial species

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