Revision of the tape used in a tape-lift protocol for DNA recovery

Bhoelai, Bryan; Beemster, Fleur; Sijen, Titia

doi:10.1016/j.fsigss.2013.10.138

Cited by 13 publications

(6 citation statements)

References 2 publications

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“…tape-lifting materials [17,18], vacuum tools [19,20], and electrostatic equipment [21], and even direct amplification [22,23]. Efficiency comparisons of various DNA-affinity bead-and filter-based extraction methods also abound [24,25].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

A review of trace “Touch DNA” deposits: Variability factors and an exploration of cellular composition

Burrill

Daniel

Frascione

2019

Forensic Science International: Genetics

122

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Section: Accepted Manuscriptmentioning

confidence: 99%

A review of trace “Touch DNA” deposits: Variability factors and an exploration of cellular composition

Burrill

Daniel

Frascione

2019

Forensic Science International: Genetics

122

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“…Even though various collection methods have been compared with each other [10][11][12][13][14], only a few studies considered the effect of variations within the stubbing technique on the final sample: the effect of tape brand [9,15] and the number of tape-liftings [9] have been studied. In the stubbing procedure that is currently used at our forensic institute, the 'stubbing force' (i.e.…”

Section: Introductionmentioning

confidence: 99%

The influence of contact force on forensic trace collection efficiency when sampling textiles with adhesive tape

Berkel

Beemster

Dankelman

et al. 2019

Forensic Science International

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DNA is a highly valuable lead to identify people who were possibly involved in a crime. Even by small contact events, minute amounts of DNA ('trace DNA') can be transferred from a DNA source to an evidentiary item, which can be enough for a successful DNA analysis. The focus of this research is to get more insight in the collection of trace DNA from textiles by 'stubbing', which is a tape-lifting method using double-sided tape placed on a stub. The relation between the 'stubbing force' (the normal force that is applied during stubbing) and the collection efficiency of microspheres is investigated.Methods: Microspheres (Ø25 mm) were used as mock traces to mimic DNA-containing micro-traces. The particles were applied to textile substrates in a suspension of ethanol that was left to evaporate before sampling. Experiments were performed on three different polyester substrates. Traces were collected by stubbing while using 5 different stubbing forces. The number of microspheres placed on each substrate was counted before sampling and all stub-tapes were analysed after sampling to count how many of the microspheres were picked up, both by using stitched images from a digital light microscope. Custommade image recognition software was used to automatically count the microspheres. Results: On all tested polyester substrates, the mean efficiency of the collection of microspheres increased with increasing stubbing force in a concave down increasing function. The increase of collection efficiency stagnated around 3-12 N, depending on the substrate material. The theoretical maximum collection efficiencies varied between 38% and 78%, depending on substrate material as well. Conclusions: Stubbing with a force higher than 12 N does not notably influence the collection efficiency from the variety of textiles that were tested. However, because the theoretical maxima of the collection efficiencies were far from 100%, it is highly likely that stubbing multiple times on the same spot of a substrate increases the total collection efficiency. The gained knowledge will help to standardize and improve the effectiveness of stubbing.

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“…Traditionally, adhesive lifters are used by crime scene investigators for trace analysis, indented handwriting analysis, latent fingerprint examinations, and shoe print lifts . Moreover, biological sample collection via tape‐lifting with a variety of adhesive lifters and tapes is widely recognized as an effective method for DNA recovery . According to their manufacturers, commercially available adhesive lifters, such as BVDA Gellifters ® , BVDA Instant Lifters ® , and Scenesafe FAST™ tape, may be amenable to visually nondestructive collection.…”

mentioning

confidence: 99%

Nondestructive Biological Evidence Collection with Alternative Swabs and Adhesive Lifters

Plaza

Mealy²,

Lane³

et al. 2015

Journal of Forensic Sciences

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In forensic science, biological material is typically collected from evidence via wet/dry double swabbing with cotton swabs, which is effective but can visibly damage an item's surface. When an item's appearance must be maintained, dry swabbing and tape-lifting may be employed as collection techniques that are visually nondestructive to substrates' surfaces. This study examined the efficacy of alternative swab matrices and adhesive lifters when collecting blood and fingerprints from glass, painted drywall, 100% cotton, and copy paper. Data were evaluated by determining the percent profile and quality score for each STR profile generated. Hydraflock(®) swabs, BVDA Gellifters(®) , and Scenesafe FAST™ tape performed as well as or better than cotton swabs when collecting fingerprints from painted drywall and 100% cotton. Collection success was also dependent on the type of biological material sampled and the substrate on which it was deposited. These results demonstrated that alternative swabs and adhesive lifters can be effective for nondestructive DNA collection from various substrates.

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Revision of the tape used in a tape-lift protocol for DNA recovery

Cited by 13 publications

References 2 publications

A review of trace “Touch DNA” deposits: Variability factors and an exploration of cellular composition

A review of trace “Touch DNA” deposits: Variability factors and an exploration of cellular composition

The influence of contact force on forensic trace collection efficiency when sampling textiles with adhesive tape

Nondestructive Biological Evidence Collection with Alternative Swabs and Adhesive Lifters

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