Revising the taxonomy of the Acinetobacter lwoffii group: The description of Acinetobacter pseudolwoffii sp. nov. and emended description of Acinetobacter lwoffii

Nemec, Alexandr; Radolfova-Krizova, Lenka; Maixnerová, Martina; Nemec, Matěj; Clermont, Dominique; Bzdil, Jaroslav; Ježek, Petr; Španělová, Petra

doi:10.1016/j.syapm.2018.10.004

Cited by 31 publications

(26 citation statements)

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“…Such models, besides allowing for species discrimination, also revealed some insights into species similarity and dissimilarity within the genus. Of note, the first PLSDA model allowed for discrimination between the ABC complex species and those belonging to the haemolytic clade, which is in agreement with the results obtained from the core genome-based tree of the Acinetobacter genus presented by Nemec et al [24]. Within the ABC complex, A. nosocomialis was grouped with A. seifertii , also in accordance with the results obtained by Nemec et al (2018).…”

Section: Discussionsupporting

confidence: 90%

“…However, A. pittii isolates were grouped with A. baumannii and A. calcoaceticus instead of being grouped with A. dijkshoorniae . The same was verified for the haemolytic clade isolates; some likely clustered in the Nemec et al [24] study but most of them clustered randomly. It should be noted that species similarity and dissimilarity presented in the Nemec work was based on the amino acid sequence evolution of the species and the results herein obtained were based on their vibrational spectra, which could justify the differences encountered among clusters.…”

Section: Discussionsupporting

confidence: 61%

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Differentiation of Taxonomically Closely Related Species of the Genus Acinetobacter Using Raman Spectroscopy and Chemometrics

2019

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In recent years, several efforts have been made to develop quick and low cost bacterial identification methods. Genotypic methods, despite their accuracy, are laborious and time consuming, leaving spectroscopic methods as a potential alternative. Mass and infrared spectroscopy are among the most reconnoitered techniques for this purpose, with Raman having been practically unexplored. Some species of the bacterial genus Acinetobacter are recognized as etiological agents of nosocomial infections associated with high rates of mortality and morbidity, which makes their accurate identification important. The goal of this study was to assess the ability of Raman spectroscopy to discriminate between 16 Acinetobacter species belonging to two phylogroups containing taxonomically closely related species, that is, the Acinetobacter baumannii-Acinetobacter calcoaceticus complex (six species) and haemolytic clade (10 species). Bacterial spectra were acquired without the need for any sample pre-treatment and were further analyzed with multivariate data analysis, namely partial least squares discriminant analysis (PLSDA). Species discrimination was achieved through a series of sequential PLSDA models, with the percentage of correct species assignments ranging from 72.1% to 98.7%. The obtained results suggest that Raman spectroscopy is a promising alternative for identification of Acinetobacter species.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 61%

Differentiation of Taxonomically Closely Related Species of the Genus Acinetobacter Using Raman Spectroscopy and Chemometrics

2019

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“…Initially they were identified as A. lwoffii based on metabolic testing and from 16S rRNA gene sequence analysis (Petrova et al, 2002; Kurakov et al, 2016; Mindlin et al, 2016). In this work, based on data from a recent article by Nemec et al (2018), revising the taxonomy of the Acinetobacter lwoffii group we showed that strain ED9-5a actually belongs to the Acinetobacter pseudolwoffii species. The remaining permafrost strains do belong to A. lwoffii .…”

Section: Methodsmentioning

confidence: 83%

Adaptive dif Modules in Permafrost Strains of Acinetobacter lwoffii and Their Distribution and Abundance Among Present Day Acinetobacter Strains

et al. 2019

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The dif /Xer system of site-specific recombination allows resolution of chromosomal dimers during bacterial DNA replication. Recently, it was also shown to be involved in horizontal transfer of a few known Xer-dependent mobile elements. Here, we show that plasmids of various Acinetobacter species, including clinically important strains, often contain multiple p dif sites that are mainly located within their accessory regions. Chromosomes of Acinetobacter strains may also contain additional dif sites, and their similarity with plasmid p dif sites is higher than with the main chromosomal site dif1 . We further identify putative mobile genetic elements containing p dif sites on both flanks of adaptive genes and analyze their distribution in Acinetobacter species. In total, we describe seven mobile elements containing genes with various adaptive functions from permafrost strains of A. lwoffii group. All of them are also spread in modern plasmids of different Acinetobacter species including A. baumannii . We could not detect p dif sites and corresponding mobile elements in closely related bacterial genera, including Psychrobacter and Moraxella . Thus, the widespread distribution of dif modules is a characteristic feature of Acinetobacter species and may contribute to their high adaptability both in the environment and in the clinic.

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“…Non-ACB-complex strains ( n = 35) comprising 21 A. lwoffii/ ‘ A. pseudolwoffii ’ (25), three A. guillouiae , three A. radioresistens, two A. beijerinckii , two A. towneri, one A. gandensis, one A. junii , one A. parvus , and one A. ursingii were not included in this study.…”

Section: Methodsmentioning

confidence: 99%

Phenotypic and Genotypic Characterization of Clinical Isolates Belonging to the Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) Complex Isolated From Animals Treated at a Veterinary Hospital in Switzerland

et al. 2019

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Objectives: We investigated a collection of strains belonging to the Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex obtained from a veterinary clinic with regard to their genetic relatedness, presence of antibiotic resistance genes and antimicrobial susceptibility profiles.Methods: Fifty-eight ACB-complex strains from animals treated at a veterinary clinic between 2006 and 2017, and seven strains collected from the hospital environment during 2012 were analyzed. Assignment to sequence types (ST) and international complexes (IC) was done by multilocus sequence typing (MLST) according to the Pasteur scheme. Genes encoding carbapenemases, aminoglycoside-modifying enzymes, macrolide-, quinolone- and co-trimoxazole resistance genes, the ISAba1 element, virulence associated intI1 genes and plasmid associated toxin-antitoxin markers were identified by microarray. Genes encoding blaOXA−51-like carbapenemases were amplified by PCR and sequenced. Susceptibility profiles were determined by disc diffusion or by broth microdilution.Results: Among 50 A. baumannii isolates from animals, two predominant clones were observed linked to CC1 (n = 27/54% of the isolates) and CC25 (n = 14/28%), respectively. Strains of IC I harbored blaOXA−69, aac(3′)-la, aadA1, sul1, intI1, and splA/T genes. Isolates belonging to CC25 possessed blaOXA−64. Six (12%) isolates belonging to CC2 and carrying blaOXA−66 were also noted. One isolate belonged to CC10 (blaOXA−68), one to CC149 (blaOXA−104), the remaining isolate was assigned to ST1220 and possessed blaOXA−116. Of six environmental A. baumannii, four (66.7%) belonged to CC25 (blaOXA−64), one (16.7%) to CC2 (blaOXA−66) and one to CC3 (blaOXA−71). Nine isolates (eight from animals and one environmental strain) were non-baumannii strains and did not harbor blaOXA−51-like genes. None of the isolates carried blaOXA−23, blaOXA−48, or blaOXA−58, and none were resistant to carbapenems.Conclusions: Clonal lineages of the veterinary A. baumannii isolates in our collection are identical to those globally emerging in humans but do not harbor blaOXA−23. A. baumannii CC25 may be specific for this particular veterinary clinic environment.

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Revising the taxonomy of the Acinetobacter lwoffii group: The description of Acinetobacter pseudolwoffii sp. nov. and emended description of Acinetobacter lwoffii

Cited by 31 publications

References 30 publications

Differentiation of Taxonomically Closely Related Species of the Genus Acinetobacter Using Raman Spectroscopy and Chemometrics

Differentiation of Taxonomically Closely Related Species of the Genus Acinetobacter Using Raman Spectroscopy and Chemometrics

Adaptive dif Modules in Permafrost Strains of Acinetobacter lwoffii and Their Distribution and Abundance Among Present Day Acinetobacter Strains

Phenotypic and Genotypic Characterization of Clinical Isolates Belonging to the Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) Complex Isolated From Animals Treated at a Veterinary Hospital in Switzerland

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