“…Genomic DNA from one adult wasp from Specimen 3 (see Table 1, aka "PrairieTET2") was extracted by TEW using Chelex 100 resin in a 30-µL volume containing 10% Chelex and 4% Protinase K (20mg/mL). The COI region for this specimen was amplified using PCR primers LCO1490 and HCO2198 as described in Triapitsyn et al (2021), with the addition of universal M13 sequencing primers. All sequencing work was done at the WSDA Entomology Lab in Olympia, WA.…”