Review on the applications of atomic force microscopy imaging in proteins

Hu, Jing; Gao, Mingyan; Wang, Zuobin; Chen, Yujuan

doi:10.1016/j.micron.2022.103293

Cited by 9 publications

(1 citation statement)

References 120 publications

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“…Essentially, the probe deflection is a function of the interaction with the sample. AFM has become an important tool in material science (Dias et al ., 2020; Last et al ., 2010; Pillet et al ., 2014; Xiao and Dufrêne, 2016) as it benefits from a nanometer resolution, low invasiveness, simplicity, and rapidity of manipulation (Hu et al ., 2022).…”

Section: Introductionmentioning

confidence: 99%

Optimization of somatic embryogenesis inEuterpe edulisMartius using auxin analogs and atomic force microscopy

Mello

Taliuli

Silva

et al. 2023

Preprint

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Euterpe edulisMartius is an endangered species of the Atlantic Forest, whose fruits have high antioxidant potential, and propagated exclusively by seeds. The present study assessed the ability of different auxin inducers and picloram analogs to trigger somatic embryogenesis inE. edulis. Immature seeds were harvested, and their zygotic embryos were excised and grown in MS culture medium supplemented with 2,4-D dichlorophenoxyacetic acid (2,4-D) or picloram at 150, 300, 450, 600 µM. The activity of picloram analogs triclopyr and clopyralid was evaluated in semisolid MS medium. At maturation and germination, picloram-derived calli and somatic embryos isolated from triclopyr-grown cultures were first transferred to pre-maturation medium and, after 30 days, to basal MS or MS medium supplemented with either 5 µM abscisic acid or 0.53 µM 1-naphthaleneacetic acid plus 12.3 µM 2-isopentenyladenine. Finally, somatic embryos with root protrusions were transferred to MS medium devoid of sucrose for 30 days and then acclimatized ex vitro. Scanning, transmission, and atomic force microscopy revealed that picloram was superior to 2,4-D but less effective than triclopyr (100 µM) in inducing embryogenesis. Maturation and germination of somatic embryos in E. edulis can be maximized by 5 µM abscisic acid, and selecting calli via atomic force microscopy.HighlightThis work opens novel roads for embryogenic induction, using a new and more efficient inducer than the usual ones, and an innovative evaluation technique based on AFM.

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Section: Introductionmentioning

confidence: 99%

Optimization of somatic embryogenesis inEuterpe edulisMartius using auxin analogs and atomic force microscopy

Mello

Taliuli

Silva

et al. 2023

Preprint

View full text Add to dashboard Cite

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pH‐induced changes in IgE molecules measured by atomic force microscopy

Hu,

Wang,

Jiang

et al. 2024

Microscopy Res & Technique

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The environment surrounding proteins is tightly linked to its dynamics, which can significantly influence the conformation of proteins. This study focused on the effect of pH conditions on the ultrastructure of Immunoglobulin E (IgE) molecules. Herein, the morphology, height, and area of IgE molecules incubated at different pH were imaged by atomic force microscopy (AFM), and the law of IgE changes induced by pH value was explored. The experiment results indicated that the morphology, height and area of IgE molecules are pH dependent and highly sensitive. In particular, IgE molecules were more likely to present small‐sized ellipsoids under acidic conditions, while IgE molecules tend to aggregate into large‐sized flower‐like structures under alkaline conditions. In addition, it was found that the height of IgE first decreased and then increased with the increase of pH, while the area of IgE increased with the increase of pH. This work provides valuable information for further study of IgE, and the methodological approach used in this study is expected to developed into AFM to investigate the changes of IgE molecules mediated by other physical and chemical factors.Research Highlights The ultrastructure of IgE molecules is pH dependent and highly sensitive. IgE molecules were tend to present small‐sized ellipsoids under acidic pH. Alkaline pH drives IgE self‐assembly into flower‐like aggregates.

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Chemical, structural and antioxidant properties of melanoidins in fish sauce

Zhu,

Wang,

Liu

et al. 2024

J Sci Food Agric

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BACKGROUNDMelanoidins, as a functional component, exhibit antioxidant properties. However, the antioxidant mechanism of melanoidins in fish sauce remains poorly understood. The present study focused on the structural characteristics, microstructure and antioxidant activity of melanoidin substances in fish sauce.RESULTSUltrafiltration and color difference analysis were utilized to confirm the main molecular weight composition of melanoidins. The ultrafiltration component > 10 kDa in fish sauce exhibited the darkest and reddest color, along with the highest content of melanoidins (699.5 mg g−1). The spectral characteristics were consistent with typical melanoidins. Gas chromatography–tandem mass spectrometry and Fourier transform infrared spectroscopy analyses revealed that the melanoidins contained a significant number of oxygen‐containing groups, amino compounds, carbohydrates, aromatic compounds and carbonyl compounds (CO), pyrrole (CN), among others. In total, 129 alkanes, 71 esters, nine olefins and two alkynes were identified. The melanoidins (> 10 kDa component) had the strongest antioxidant activity, including a reducing power of 0.8, 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) radical scavenging activity of 67.7% and 2,2‐diphenyl‐1‐picrylhydrazyl radical scavenging activity of 92.4%. Additionally, the melanoidins (> 10 kDa component) had the highest total phenolic content at 48.2 μg gallic acid equivalents mL−1.CONCLUSIONMelanoidins are important factors affecting the antioxidant activity of fish sauce. There were differences in the structural properties of melanoidin fractions with different molecular weights. © 2024 Society of Chemical Industry.

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Review on the applications of atomic force microscopy imaging in proteins

Cited by 9 publications

References 120 publications

Optimization of somatic embryogenesis inEuterpe edulisMartius using auxin analogs and atomic force microscopy

Optimization of somatic embryogenesis inEuterpe edulisMartius using auxin analogs and atomic force microscopy

pH‐induced changes in IgE molecules measured by atomic force microscopy

Chemical, structural and antioxidant properties of melanoidins in fish sauce

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