Review on Simultaneous Equation Method (Vierodt’s Method)

Vyas, Amitkumar J.; Jha, Shalini Anilkumar; Patel, Ashok B.; Shah, Sangita; Sheth, Devang

doi:10.52711/2231-5675.2022.00026

Cited by 10 publications

(2 citation statements)

References 29 publications

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“…In RP-HPLC, there are two phases: a polar mobile phase and a non-polar stationary phase. The parameters vary during the development process according to the size of the column, the characteristics of the apparatus, and the wavelength at which the asymmetric peaks and mobile phase are present (Yadav et al, 2017;Vyas et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

RP-HPLC Technique for Dustasteride Quantitative Estimation in Tablet Dosage Form: Formulation and Validation of Analytical Technique

Rajaram,

Erattil

et al. 2024

Intern. J. Bio. Env. Invest.

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RP-HPLC is a novel analytical technique that can be used to detect dutasteride in pharmaceutical formulations and bulk pharmaceuticals. The aim of developing this technique was to provide an analytical RP HPLC method based on the Shiseido C18 analytical column that is accurate, dependable, and analytical for determining the concentration of dutasteride in both bulk and single component forms. The phase of mobile phase MeOH: ACN: H2O ratio was 75:10:15 (V/V/V) at a UV detector wavelength of 274 nm, and the flow rate remained constant at 0.7 ml/min. At 8:34 min, the dutasteride case was concluded amicably. The run lasted 10 min, with the temperature remaining at 20°C. Tests were conducted to ensure that the recently developed analytical RP-HPLC technology satisfies the reliability requirements. In compliance with the ICH Revised Q2 (R1) guidelines for analytical technique validation, it was verified. The analytical technique's method features demonstrated its ability to maintain its sensitivity, accuracy, precision, selectivity, and consistency throughout time. A straight line relationship between the concentration range of 10 to 22 ppm was displayed in the calibration plot. 5.3010 µg/ml was the LOQ, while 10.999 µg/ml was the LOD. The drug's consistency test indicated that the procedure is most likely rather excellent. Reliability tests revealed that the %RSD was within allowable bounds. The authorised technique quantified dutasteride in pharmaceutical formulations and bulk drugs satisfactorily.

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Section: Introductionmentioning

confidence: 99%

RP-HPLC Technique for Dustasteride Quantitative Estimation in Tablet Dosage Form: Formulation and Validation of Analytical Technique

Rajaram,

Erattil

et al. 2024

Intern. J. Bio. Env. Invest.

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“…Analytical quality by design (AQbD) and CCD help in regulatory compliance for RP-HPLC method development, stress testing, or stability-indicating methods (3)(4)(5)(6). UV spectrophotometric methods (simultaneous equation method/ Vierodt') and LC-MS are widely acceptable for the simultaneous estimation of pharmaceutical combinations (7)(8)(9). The presence of impurities or degradants critically affects the stability and pharmacological action of pharmaceutical API and drug products (10)(11)(12)(13)(14).…”

Section: Introductionmentioning

confidence: 99%

Stability Indicating RP-HPLC Method Development and Validation for Simultaneous Estimation of Dapagliflozin Propanediol Monohydrate and Teneligliptin Hydrobromide Hydrate in Synthetic Mixture

HITEKSHA,

CHOTALİYA

2023

Journal of the Turkish Chemical Society Section A: Chemistry

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For the quantitative measurement of Dapagliflozin propanediol monohydrate and Teneligliptin hydrobromide hydrate in synthetic mixture form in the presence of its degradants, precise, accurate, robust, cost-effective, and isocratic stability indicating RP-HPLC method was developed and validated. The mobile phase comprises [Methanol: 20 mM Ammonium formate (70:30 v/v)] at a flow rate of 1.0 ml/min, injection volume of 20 µl, and UV detection at 225 nm. Separation was accomplished using Gemini, C18 column. Teneligliptin hydrobromide hydrate and Dapagliflozin propanediol monohydrate were eluted with retention times of 6.65 minutes and 4.20 minutes, respectively. This procedure was approved following ICH guideline Q2 (R1). The calibration plots for Dapagliflozin propanediol monohydrate and Teneligliptin hydrobromide hydrate have correlation coefficients of 0.9995 and 0.9996 over the concentration ranges of 5-100 µg/ml and 10-200 µg/ml respectively. For Dapagliflozin propanediol monohydrate and Teneligliptin hydrobromide hydrate, accuracy ranged from 99.81-100.78% and 99.13-100.69%, respectively. For Dapagliflozin propanediol monohydrate and Teneligliptin hydrobromide hydrate, the LOD was found to be 0.947 µg/ml and 1.355 µg/ml. In contrast, the LOQ was 2.869 µg/ml and 4.107 µg/ml, respectively. The findings demonstrated the applicability of the devised approach for routine analysis of Teneligliptin hydrobromide hydrate and Dapagliflozin propanediol monohydrate in a synthetic mixture form with its degradants.

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RP-HPLC Method Development for Simultaneous Estimation of Brimonidine Tartrate and Timolol Maleate in Ophthalmic Preparation

Patel,

Mandavia,

Vyas

et al. 2023

PCI- Approved-IJPSN

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Introduction: A simple, precise, rapid, and reproducible RP-HPLC method is developed for the simultaneous estimation of brimonidine tartrate and timolol maleate present in ophthalmic dosage forms. Method: Gradient RP-HPLC is carried out at 35℃ on column C18 (25 cm X 4.6 mm, 5 µm) with mobile phase a ACN and buffer (25 ml ACN & 475 ml buffer) and mobile phase B water and ACN (37.5 ml water & 212.5 ml ACN) at a flow rate of 1.2ml/min. detection is carried out using DAD detector at 248 nm. Parameter such as linearity, accuracy, precision, specificity, LOD, LOQ and robustness are studied as per ICH guidelines Q2 (R1). Results: The linearity range of Brimonidine tartrate and Timolol maleate are 10-50 µg/ml and 4-20 µg/ml. The accuracy for both Brimonidine Tartrate and Timolol Maleate are in range of 100-101%. The RSD for Brimonidine Tartrate and Timolol Maleate are less than 2 for precision, specificity, robustness respectively. Conclusion: This RP-HPLC method can be highly suitable for analysis of brimonidine tartrate and timolol maleate without interference in combined dosage form.

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Review on Simultaneous Equation Method (Vierodt’s Method)

Cited by 10 publications

References 29 publications

RP-HPLC Technique for Dustasteride Quantitative Estimation in Tablet Dosage Form: Formulation and Validation of Analytical Technique

RP-HPLC Technique for Dustasteride Quantitative Estimation in Tablet Dosage Form: Formulation and Validation of Analytical Technique

Stability Indicating RP-HPLC Method Development and Validation for Simultaneous Estimation of Dapagliflozin Propanediol Monohydrate and Teneligliptin Hydrobromide Hydrate in Synthetic Mixture

RP-HPLC Method Development for Simultaneous Estimation of Brimonidine Tartrate and Timolol Maleate in Ophthalmic Preparation

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