Review on recent advances in the analysis of isolated organelles

Satori, Chad P.; Košťál, Vratislav; Arriaga, Edgar A.

doi:10.1016/j.aca.2012.09.041

Cited by 52 publications

(39 citation statements)

References 113 publications

(155 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is also the reason for us to use sodium-containing medium for the isolation and preparation of mitochondria in our current and our recent study [8]. Actually, the protocol or procedure employed in our current and our recent study [8] using sodium appears better in maintaining the genetic structure and function of isolated mitochondria [29, 30, 31]. …”

Section: Discussionmentioning

confidence: 99%

Mitochondrial transplantation attenuates hypoxic pulmonary hypertension

et al. 2016

View full text Add to dashboard Cite

Mitochondria are essential for the onset of hypoxia-induced pulmonary vasoconstriction and pulmonary vascular-remodeling, two major aspects underlying the development of pulmonary hypertension, an incurable disease. However, hypoxia induces relaxation of systemic arteries such as femoral arteries and mitochondrial heterogeneity controls the distinct responses of pulmonary versus femoral artery smooth muscle cells to hypoxia in vitro. The aim of this study was to determine whether mitochondrial heterogeneity can be experimentally exploited in vivo for a potential treatment against pulmonary hypertension. The intact mitochondria were transplanted into Sprague-Dawley rat pulmonary artery smooth muscle cells in vivo via intravenous administration. The immune-florescent staining and ultrastructural examinations on pulmonary arteries confirmed the intracellular distribution of exogenous mitochondria and revealed the possible mitochondrial transfer from pulmonary artery endothelial cells into smooth muscle cells in part through their intercellular space and intercellular junctions. The transplantation of mitochondria derived from femoral artery smooth muscle cells inhibited acute hypoxia-triggered pulmonary vasoconstriction, attenuated chronic hypoxia-induced pulmonary vascular remodeling, and thus prevented the development of pulmonary hypertension or cured the established pulmonary hypertension in rats exposed to chronic hypoxia. Our findings suggest that mitochondrial transplantation possesses potential implications for exploring a novel therapeutic and preventive strategy against pulmonary hypertension.

show abstract

Section: Discussionmentioning

confidence: 99%

Mitochondrial transplantation attenuates hypoxic pulmonary hypertension

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Another consideration used to define the scope of this review was previous reviews related to subcellular analysis that were published on sub-themes such as the technologies used to analyze isolated organelles 90 or prepare subcellular fractions for proteomic analysis. 91 For the interested reader, Table 2 compiles topical, specialized reviews that were published recently.…”

Section: Introductionmentioning

confidence: 99%

Bioanalysis of Eukaryotic Organelles

et al. 2013

Self Cite

View full text Add to dashboard Cite

“…Early [77][78][79][80] and modern [81][82][83] original research papers and reviews [84][85][86][87] describe a large amount of work devoted to the separation of cells, vesicles, organelles and bacteria.…”

Section: Biological Samplesmentioning

confidence: 99%