Review on microbial enhanced oil recovery in China: mechanisms, potential disadvantages, and application of genetic engineering

Bian, Ziwei; Zhang, Xiangchun; Wu, Yiying; Wei, Lusha; Yifei, Wu; Wu, Haijun

doi:10.1080/15567036.2021.1897192

Cited by 4 publications

(1 citation statement)

References 57 publications

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“…Compared to traditional EOR technologies, MEOR is regarded as a low-cost and environmentally friendly technology [12]. Additionally, numerous studies have demonstrated its superior economic efficiency [13]. Microbial metabolites (e.g., biosurfactants, biopolymers, gas, acids, and solvents) can enhance crude oil fluidity and improve water spread efficiency by changing a reservoir's wettability, reducing crude oil viscosity, degrading heavy components, and reducing interfacial tension (IFT).…”

Section: Introductionmentioning

confidence: 99%

Horizontal and Vertical Comparison of Microbial Community Structures in a Low Permeability Reservoir at the Local Scale

Zhi,

Bian,

Chen

et al. 2023

Microorganisms

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Petroleum microorganisms play a crucial role in the application of microbial-enhanced oil recovery, and the community structures of petroleum microorganisms have been widely studied. Due to variations in reservoir geological conditions, reservoir microbial communities exhibit unique characteristics. However, previous studies have primarily focused on microbial community changes within a single well, a single block, and before and after water flooding, and thus, cross-horizon and cross-regional comparative studies of in situ microbial communities are lacking. In this study, the 16S rRNA full-length sequencing method was adopted to study bacterial communities in crude oil samples taken from two wells at the same depths (depths of 2425 m and 2412 m) but approximately 20 km apart in the Hujianshan oilfield, located in the Ordos Basin. At the same time, the results were combined with another layer of research data from another article (from a depth of 2140 m). The aim was to compare the differences in the microbial community structures between the oil wells on a horizontal scale and a vertical scale. The results revealed that there were minimal differences in the microbial community structures that were influenced by the horizontal distances within a small range (<20 km), while differences were observed at a larger spatial scale. However, the dominant bacteria (Proteobacteria and Bacteroidetes) in the different oilfields were similar. Vertical depth variations (>300 m) had significant impacts on the communities, and this was mainly controlled by temperature. The greater the depth, the higher formation temperature, leading to an increase in thermophilic and anaerobic bacteria within a community.

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Section: Introductionmentioning

confidence: 99%

Horizontal and Vertical Comparison of Microbial Community Structures in a Low Permeability Reservoir at the Local Scale

Zhi,

Bian,

Chen

et al. 2023

Microorganisms

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Recent advances in the application of nanoparticles: focus on enhanced oil recovery

Bernuy,

Mendiburu,

Huallpachoque

et al. 2024

J Braz. Soc. Mech. Sci. Eng.

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Construction and Performance Evaluation of Dual-Metabolite Oil-Producing Engineering Bacteria Suitable for Low-Permeability Reservoir

Yang

Guo

et al. 2022

Lithosphere

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Microbial oil recovery (MEOR) technology has a wide application prospect in the field of enhanced oil recovery in low-permeability reservoirs, and genetic engineering plays an important role. At present, oil-production engineering bacteria constructed by genetic engineering are mainly used to obtain high metabolites and strong environmental adaptability; there are few reports on genetically engineered bacteria with different functional metabolites. However, the bacteria which could efficiently produce one metabolite have a poor ability to produce other metabolites. Therefore, in order to reduce the cost of nutrients and improve the effect of microbial oil recovery, dual-metabolite bacteria have been constructed for low-permeability reservoir. In this paper, four rhamnolipid expression plasmids are extracted from E. coli-produced biosurfactant. Then, the rhamnolipid expression plasmids are transferred into the bacteria-produced biopolymer by electrotransformation, and the metabolite performance of the constructed bacteria is evaluated. Finally, the profile control and displacement performance of the constructed bacteria are studied through low-permeability core experiments. The experimental results show that three bacteria, WS1, WS2, and WS3, are successfully constructed that have preferable ability, among which the bacteria WS2 has the best capacity for producing biopolymer and biosurfactant at the same time. After culturing 96 h, the viscosity of the bacteria WS2 fermentation broth could increase to 42.1 mPa·s, and the surface tension and interfacial tension of the fermentation broth could decrease to 24.3 mN/m and 0.035 mN/m, respectively. This time-varying biological viscosity ensures that the microbial system can enter the low-permeability reservoir at a low injection pressure, and the oil recovery of the low-permeability core could be increased by 10.18% after injecting 0.5 PV WS2 microbial system. The findings of this study can help for better understanding of gene construction and technical support for further popularization and application of MEOR in low-permeability reservoirs.

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Review on microbial enhanced oil recovery in China: mechanisms, potential disadvantages, and application of genetic engineering

Cited by 4 publications

References 57 publications

Horizontal and Vertical Comparison of Microbial Community Structures in a Low Permeability Reservoir at the Local Scale

Horizontal and Vertical Comparison of Microbial Community Structures in a Low Permeability Reservoir at the Local Scale

Recent advances in the application of nanoparticles: focus on enhanced oil recovery

Construction and Performance Evaluation of Dual-Metabolite Oil-Producing Engineering Bacteria Suitable for Low-Permeability Reservoir

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