Review on Complete Mueller Matrix Optical Scanning Microscopy Imaging

Gratiet, Aymeric Le; Mohebi, Ali; Callegari, Fabio; Bianchini, Paolo; Diaspro, Alberto

doi:10.3390/app11041632

Cited by 25 publications

(13 citation statements)

References 74 publications

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“…Another optical polarization-based measurement method that is used for studying peptides’ and amino acids’ micro- and nanostructures is polarized optical microscopy (POM) [ 50 , 51 ]. Depending on the setup and components, one can obtain quantitative information with respect to the SAPA architectures (such as the 16 elements of a Mueller matrix [ 52 ], retardation, refractive index, thickness, and birefringence [ 50 ]) and also polarization imaging information.…”

Section: Polarization-based Measurement Methods For Characterization ...mentioning

confidence: 99%

Optical Polarization-Based Measurement Methods for Characterization of Self-Assembled Peptides’ and Amino Acids’ Micro- and Nanostructures

Handelman

2022

Molecules

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In recent years, self-assembled peptides’ and amino acids’ (SAPA) micro- and nanostructures have gained much research interest. Here, description of how SAPA architectures can be characterized using polarization-based optical measurement methods is provided. The measurement methods discussed include: polarized Raman spectroscopy, polarized imaging microscopy, birefringence imaging, and fluorescence polarization. An example of linear polarized waveguiding in an amino acid Histidine microstructure is discussed. The implementation of a polarization-based measurement method for monitoring peptide self-assembly processes and for deriving molecular orientation of peptides is also described.

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Section: Polarization-based Measurement Methods For Characterization ...mentioning

confidence: 99%

Optical Polarization-Based Measurement Methods for Characterization of Self-Assembled Peptides’ and Amino Acids’ Micro- and Nanostructures

Handelman

2022

Molecules

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“…A way to overcome such issues has been demonstrated by polarization-based microscopy techniques commonly used to enhance contrasts discerning polarimetric characteristics of anisotropic structures and chiral organization of macromolecules 9 . The technique based on employing Mueller–Stokes polarimetry formalism that confers a complete description of the optical properties of sample 10 – 12 . The polarimetry method linked to dichroism and birefringence of the specimen 13 , 14 .…”

Section: Introductionmentioning

confidence: 99%

Phasor map analysis to investigate Hutchinson–Gilford progeria cell under polarization-resolved optical scanning microscopy

Mohebi

Gratiet

Trianni

et al. 2022

Sci Rep

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Polarized light scanning microscopy is a non-invasive and contrast-enhancing technique to investigate anisotropic specimens and chiral organizations. However, such arrangements suffer from insensitivity to confined blend of structures at sub-diffraction level. Here for the first time, we present that the pixel-by-pixel polarization modulation converted to an image phasor approach issues an insightful view of cells to distinguish anomalous subcellular organizations. To this target, we propose an innovative robust way for identifying changes in the chromatin compaction and distortion of nucleus morphology induced by the activation of the lamin-A gene from Hutchinson–Gilford progeria syndrome that induces a strong polarization response. The phasor mapping is evaluated based on the modulation and phase image acquired from a scanning microscope compared to a confocal fluorescence modality of normal cell opposed to the progeria. The method is validated by characterizing polarization response of starch crystalline granules. Additionally, we show that the conversion of the polarization-resolved images into the phasor could further utilized for segmenting specific structures presenting various optical properties under the polarized light. In summary, image phasor analysis offers a distinctly sensitive fast and easy representation of the polarimetric contrast that can pave the way for remote diagnosis of pathological tissues in real-time.

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“…MM microscopy has been successfully used in numerous research fields such as materials science (6), biomedical diagnostics (7)(8)(9), and microscopic imaging (10-13), showcasing its versatility and easy implementation on multimodal systems (14). To highlight a particular physical process, decomposing the MM is a mandatory step for tracking the amplitude of a specific polarimetric effect.…”

Section: Introductionmentioning

confidence: 99%

Phasor approach of Mueller matrix optical scanning microscopy for biological tissue imaging

Gratiet

Lanzanò

Bendandi

et al. 2021

Biophysical Journal

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Mueller matrix microscopy is an advanced imaging technique providing a full characterization of the optical polarization fingerprint of a sample. The Lu-Chipman (LC) decomposition, a method based on the modeling of elementary polarimetric arrangements and matrix inversions, is the gold standard to extract each polarimetric component separately. However, this models the optical system as a small number of discrete optical elements and requires a priori knowledge of the order in which these elements occur. In stratified media or when the ordering is not known, the interpretation of the LC decomposition becomes difficult. In this work, we propose a new, to our knowledge, representation dedicated to the study of biological tissues that combines Mueller matrix microscopy with a phasor approach. We demonstrate that this method provides an easier and direct interpretation of the retardance images in any birefringent material without the use of mathematical assumptions regarding the structure of the sample and yields comparable contrast to the LC decomposition. By validating this approach through numerical simulations, we demonstrate that it is able to give access to localized structural information, resulting in a simple determination of the birefringent parameters at the microscopic level. We apply our novel, to our knowledge, method to typical biological tissues that are of interest in the field of biomedical diagnosis.

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Review on Complete Mueller Matrix Optical Scanning Microscopy Imaging

Cited by 25 publications

References 74 publications

Optical Polarization-Based Measurement Methods for Characterization of Self-Assembled Peptides’ and Amino Acids’ Micro- and Nanostructures

Optical Polarization-Based Measurement Methods for Characterization of Self-Assembled Peptides’ and Amino Acids’ Micro- and Nanostructures

Phasor map analysis to investigate Hutchinson–Gilford progeria cell under polarization-resolved optical scanning microscopy

Phasor approach of Mueller matrix optical scanning microscopy for biological tissue imaging

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