Review of the Comparative Susceptibility of Microbial Species to Photoinactivation Using 380–480 nm Violet‐Blue Light

Tomb, Rachael; White, Tracy; Coia, John; Anderson, John G.; MacGregor, S.J.; Maclean, Michelle

doi:10.1111/php.12883

Cited by 68 publications

(62 citation statements)

References 110 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Inactivation was claimed by Halstead et al as well, who investigated 400 nm light against 34 isolates and found violet light effective against P. aeruginosa and S. aureus [53]. Based on these findings and the literature, there is no doubt about the antibacterial activity of the violet light [32,40,41,43,49,51,54,56,[63][64][65][66][67]. However, the evaluation of its effectiveness and a direct comparison of the results, obtained by different studies, are more complicated.…”

Section: Violet Lightmentioning

confidence: 93%

“…In some works, blue light (420-470 nm) has been tested as well, showing a strong effect against both Gram-positive (Bacillus cereus, Listeria monocytogenes and methicillin-resistant S. aureus) and Gram-negative pathogens (P. aeruginosa, Salmonella Typhimurium and E. coli) in their planktonic form [39][40][41]49,50,54,56,[65][66][67][68]. However, Abana et al claimed that the effect of 455 nm is not completely bactericidal on E. coli but depends on the microorganism (pathogenic or non-pathogenic) and growth phase.…”

Section: Blue Lightmentioning

confidence: 99%

“…The damage mechanism is due to the tail of the violet LED spectrum continuing in the UV-A spectral region, which is responsible for their destruction [71]. Finally, the last factor that may play a role in the detrimental effect of light on P. fluorescens biofilms is its effect on the production of pyocyanin or pyoverdine (siderophores), blue extracellular products that may contribute to light absorption [67,72].…”

Section: Factors Influencing the Inactivation Kineticsmentioning

confidence: 99%

See 2 more Smart Citations

Visible Light as an Antimicrobial Strategy for Inactivation of Pseudomonas fluorescens and Staphylococcus epidermidis Biofilms

et al. 2020

View full text Add to dashboard Cite

The increase of antimicrobial resistance is challenging the scientific community to find solutions to eradicate bacteria, specifically biofilms. Light-Emitting Diodes (LED) represent an alternative way to tackle this problem in the presence of endogenous or exogenous photosensitizers. This work adds to a growing body of research on photodynamic inactivation using visible light against biofilms. Violet (400 nm), blue (420 nm), green (570 nm), yellow (584 nm) and red (698 nm) LEDs were used against Pseudomonas fluorescens and Staphylococcus epidermidis. Biofilms, grown on a polystyrene surface, were irradiated for 4 h. Different irradiance levels were investigated (2.5%, 25%, 50% and 100% of the maximum irradiance). Surviving cells were quantified and the inactivation kinetic parameters were estimated. Violet light could successfully inactivate P. fluorescens and S. epidermidis (up to 6.80 and 3.69 log10 reduction, respectively), while blue light was effective only against P. fluorescens (100% of maximum irradiance). Green, yellow and red irradiation neither increased nor reduced the biofilm cell density. This is the first research to test five different wavelengths (each with three intensities) in the visible spectrum against Gram-positive and Gram-negative biofilms. It provides a detailed study of the potential of visible light against biofilms of a different Gram-nature.

show abstract

Section: Violet Lightmentioning

confidence: 93%

Section: Blue Lightmentioning

confidence: 99%

Section: Factors Influencing the Inactivation Kineticsmentioning

confidence: 99%

See 1 more Smart Citation

Visible Light as an Antimicrobial Strategy for Inactivation of Pseudomonas fluorescens and Staphylococcus epidermidis Biofilms

et al. 2020

View full text Add to dashboard Cite

show abstract

“…An in vitro study evaluating the efficacy of violet 405nm light in treating ampicillin resistant Escherichia coli found an 81.7% reduction of bacterial growth with irradiance of 2.89 mW/cm 2 over 120 min (p < 0.001) [56]. Similarly, a review of both Gram-positive and Gram-negative bacteria susceptibility to violet light therapy between 380 and 480 nm found a 91% inactivation of Pseudomonas aeruginosa with 405 nm of VLT [57]. Finally, heat has been shown to be effective in disrupting the microbial membrane and therefore killing bacteria.…”

Section: Other Therapeutic Approachesmentioning

confidence: 99%

Trends, Epidemiology, and Management of Multi-Drug Resistant Gram-Negative Bacterial Infections in the Hospitalized Setting

2020

View full text Add to dashboard Cite

The increasing prevalence of antibiotic resistance is a threat to human health, particularly within vulnerable populations in the hospital and acute care settings. This leads to increasing healthcare costs, morbidity, and mortality. Bacteria rapidly evolve novel mechanisms of resistance and methods of antimicrobial evasion. Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Acinetobacter baumannii have all been identified as pathogens with particularly high rates of resistance to antibiotics, resulting in a reducing pool of available treatments for these organisms. Effectively combating this issue requires both preventative and reactive measures. Reducing the spread of resistant pathogens, as well as reducing the rate of evolution of resistance is complex. Such a task requires a more judicious use of antibiotics through a better understanding of infection epidemiology, resistance patterns, and guidelines for treatment. These goals can best be achieved through the implementation of antimicrobial stewardship programs and the development and introduction of new drugs capable of eradicating multi-drug resistant Gram-negative pathogens (MDR GNB). The purpose of this article is to review current trends in MDR Gram-negative bacterial infections in the hospitalized setting, as well as current guidelines for management. Finally, new and emerging antimicrobials, as well as future considerations for combating antibiotic resistance on a global scale are discussed.

show abstract

“…Photodynamic inactivation of vegetative bacteria and spores by high-intensity visible light within the blue range of the spectrum at wavelengths of ϳ400 nm has recently garnered significant interest due to the intrinsic antimicrobial characteristics of blue light (17)(18)(19). Significant activity has been demonstrated against Bacillus and Clostridium species; however, spores require a significantly larger dose of blue light to mediate killing than do vegetative cells (20).…”

mentioning

confidence: 99%

Role of DNA Repair and Protective Components in Bacillus subtilis Spore Resistance to Inactivation by 400-nm-Wavelength Blue Light

Djouiai

Thwaite

Laws

et al. 2018

Appl Environ Microbiol

View full text Add to dashboard Cite

The high intrinsic decontamination resistance of Firmicutes spores, is important medically (disease) and commercially (food spoilage). Effective methods of spore eradication would be of considerable interest in the health care and medical products industries; particularly if the decontamination method effectively killed spores while remaining benign to both humans and sensitive equipment. Intense blue light at ∼ 400 nm is one such treatment that has drawn significant interest. This work has determined the resistance of spores to blue light in an extensive panel of strains, including wild-type strains and mutants that: i) lack protective components such as the spore coat and its pigment (s) or the DNA protective α/β-type small acid-soluble spore proteins (SASP); ii) have an elevated spore core water content; or iii) lack enzymes involved in DNA repair, including homologous recombination and non-homologous end joining (HR and NHEJ), apurinic/apyrimidinic endonucleases, nucleotide- and base excision repair (NER& BER), translesion synthesis (TLS) by Y-family DNA polymerases and spore photoproduct (SP) removal by SP lyase (SPL). The most important factors in spore blue light resistance were determined to be: spore coats/pigmentation, α/β-type SASP, NER, BER TLS and SP repair. A major conclusion from this work is that blue light kills spores by DNA-damage, and the results in this work indicate at least some of the specific DNA-damage. It appears that high-intensity blue light could be a significant addition to the agents used to kill bacterial spores in applied settings.Effective methods of spore inactivation would be of considerable interest in the health care and medical products industries; particularly if the decontamination method effectively killed spores while remaining benign to both humans and sensitive equipment. Intense blue light radiation is one such treatment that has drawn significant interest. In this work in a systematic fashion all known spore protective features, as well as universal and spore-specific DNA repair mechanisms were tested on their contribution to the resistance of spores to blue light radiation.

show abstract

Review of the Comparative Susceptibility of Microbial Species to Photoinactivation Using 380–480 nm Violet‐Blue Light

Cited by 68 publications

References 110 publications

Visible Light as an Antimicrobial Strategy for Inactivation of Pseudomonas fluorescens and Staphylococcus epidermidis Biofilms

Visible Light as an Antimicrobial Strategy for Inactivation of Pseudomonas fluorescens and Staphylococcus epidermidis Biofilms

Trends, Epidemiology, and Management of Multi-Drug Resistant Gram-Negative Bacterial Infections in the Hospitalized Setting

Role of DNA Repair and Protective Components in Bacillus subtilis Spore Resistance to Inactivation by 400-nm-Wavelength Blue Light

Contact Info

Product

Resources

About