Review of Secondary Causes of Osteoporotic Fractures Due to Diabetes and Spinal Cord Injury

Zamarioli, Ariane; Staut, Caio de Andrade; Volpon, José Batista

doi:10.1007/s11914-020-00571-w

Cited by 6 publications

(12 citation statements)

References 83 publications

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“…Osteoporosis (OP), one of the common bone metabolic diseases in clinic, is characterized by changes in the bone microstructure and reduction in bone mass per unit volume, which is more common in elderly men and postmenopausal women [ 1 , 2 ]. OP can easily reduce the strength and increase the brittleness of bones and greatly increase the possibility of fractures in patients.…”

Section: Introductionmentioning

confidence: 99%

Expression of lncRNA MALAT1 through miR-144-3p in Osteoporotic Tibial Fracture Rats and Its Effect on Osteogenic Differentiation of BMSC under Traction

Ling¹,

Xu²,

Sun

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Objective. To investigate the expression of lncRNA MALAT1 and miR-144-3p in osteoporotic (OP) tibial fracture rats and analyze their targeting relationship and effects on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSC) under traction. Methods. The OP tibial fracture model was established, and the rats were divided into a sham group and a model group. The tibial tissue of these rats was taken. BMSC of cultured rats with good growth was purchased and grouped according to the presence or absence of transfection of si-MALAT1 and miR-144-3p-mimic. The expression of MALAT1 and miR-144-3p in each group was detected. The bioinformatics website and double luciferase were used to predict the targeting relationship between MALAT1 and miR-144-3p and to detect the expression of genes related to bone differentiation (collagen I, osteocalcin (OCN), osteopontin (OPN), and alkaline phosphatase (ALP)) of each component, and ALP staining and AR staining were used to detect the formation of BMSC calcium nodules. Results. The levels of ALP and TRAP in the model group were higher than that in the sham group ( P < 0.05 ). qRT-PCR results showed that the relative expression level of MALAT1 in the model group was higher than that in the sham group, and the relative expression level of miR-144-3p was lower than that in the sham group ( P < 0.05 ). MALAT1 has a targeting relationship with miR-144-3p. qRT-PCR results showed that the relative expression level of MALAT1 in the tension-MSC group was higher than the MSC group, and the relative expression level of miR-144-3p was lower than the MSC group ( P < 0.05 ). The expressions of collagen I, OCN, OPN, and ALP proteins in the si-MALAT1 group were higher than those of the si-NC group ( P < 0.05 ). The results of ALP staining showed that BMSCs of the si-MALAT1 group had stronger osteogenic differentiation capacity and higher ALP activity than those of the si-NC group. The results of AR staining showed that compared with the si-NC group, the mineralization degree of cells in the si-MALAT1 group was higher, the number of calcium nodules was more, and the cells were more deeply stained. The expressions of collagen I, OCN, OPN, and ALP proteins in the miR-144-3p-mimic group were higher than the mimic-NC group ( P < 0.05 ). ALP staining results showed that BMSCs in the miR-144-3p-mimic group had strong osteogenic differentiation capacity and high ALP activity compared with the mimic-NC group. The results of AR staining showed that, compared with the mimic-NC group, the mineralization degree of cells in the miR-144-3p-mimic group was higher, the number of calcium nodules was more and the cells were more deeply stained. Conclusion. In the OP rat model with the tibial fracture, the expression of MALAT1 is upregulated and that of miR-144-3p is downregulated. MALAT1 has a targeting relationship with miR-144-3p, and downregulation of MALAT1 and upregulation of miR-144-3p can promote the osteogenic differentiation of BMSC under traction.

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Section: Introductionmentioning

confidence: 99%

Expression of lncRNA MALAT1 through miR-144-3p in Osteoporotic Tibial Fracture Rats and Its Effect on Osteogenic Differentiation of BMSC under Traction

Ling¹,

Xu²,

Sun

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…In this regard, one of the participants stated: “…at the homes of some patients, toilets have stairs, it would be great if an additional stairs-free bathroom on a flat surface can be built for them…“[ 3 ]. Another participant recalled taking his patient to a park: “…unfortunately, there was no device in the park that a SCI patient could use…” [ 4 ]. The participants specified that there should be specially designed parks for patients with SCI.…”

Section: Community Reintegrationmentioning

confidence: 99%

“…The participants specified that there should be specially designed parks for patients with SCI. Addressing this issue, one of the caregivers noted: “…they could have at least built a special park for them…” [ 4 ]. Another participant stated that furniture had unsuitable design for these patients, “…there is no adaptation in furniture, stairs, …” [ 5 ].…”

Section: Community Reintegrationmentioning

confidence: 99%

“…Data analysis showed that the care services aiming to empower patients should be initiated from the first days of injury development so that patients can experience better outcomes. One of the participants addressed the ramifications of the delayed starting of care provision to SCI patients: “…we had a client who was referred to us ten years after the injury, when he developed deformation, deterioration, and osteoporosis along with numerous other acute illnesses, it is very difficult for such a person to return to normal life, and there is not much that one can do…” [ 4 ]. The data indicated that patients might gradually lose their spirit for complying with rehabilitation programs.…”

Section: Community Reintegrationmentioning

confidence: 99%

“…Spinal cord injury causes dysfunction in various body systems such as the sensory, motor, musculoskeletal, digestive, urinary, cutaneous, and respiratory systems, as well as psychological disorders [ 4 , 5 ]. These consequences impose a great work burden on the family and the health system [ 6 ] and require spending high costs for managing the patient [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

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Exploring caregivers’ perceptions of community-based service requirements of patients with spinal cord injury: a qualitative study

Galehdar

Heydari

2023

BMC Prim. Care

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Background The incidence of spinal cord injury is increasing worldwide. Patients with spinal cord injury and their families face many difficulties during the disease course. Caregivers are more involved with these patients than anyone else, so recognizing patients’ care requirements based on caregivers’ opinions can facilitate care provision to these people. The purpose of this study was to explore caregivers’ perceptions of the community-based services requirements of patients with spinal cord injury. Methods This qualitative research was conducted in Iran from Apr 2021 to Dec 2022 using the conventional content analysis method. The participants in the study included family caregivers and providers of home care services to patients with spinal cord injury, who were selected by purposeful sampling. Data were collected by conducting 14 face-to-face interviews and analyzed based on the method proposed by Lundman and Graneheim. Results Data analysis led to the extraction of 815 primary codes, which were organized into two themes: community reintegration (with two categories, including the need to provide a suitable social platform and lifelong care) and palliative care (with two categories, including family conference and survival management). Conclusion Social facilities and infrastructure should be modified in a way that patients with spinal cord injury can appropriately benefit from community-based care services and an independent satisfactory life. Palliative care should be continuously provided from the time of lesion development until the patient’s death.

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Effect of robotic gait training on muscle and bone characteristics in spinal cord transected rats

LeBlanc,

Soucy,

Moustafa‐Bayoumi

et al. 2024

Journal Orthopaedic Research

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Osteoporosis and loss of muscle mass are secondary issues with spinal cord injury. Robotic gait training has provided evidence of increasing bone density and muscle mass, but its effect on bone strength is undetermined. The purpose of this study was to determine the effect of a 6‐week robotic locomotion training program on skeletal muscle mass and bone characteristics. Twelve female Sprague‐Dawley rats received a mid‐thoracic spinal cord transection at 5 days old and at 3 weeks old were assigned to a Control or Trained Group. The Trained Group performed 5‐min sessions on the Rat Stepper 5 days a week for 6 weeks with 90% of body weight supported. At the end of the 6 weeks, body mass was obtained and right femurs and four lower extremity muscles were harvested. Femur bone mineral density was measured with DXA and mechanical characteristics of the femur were determined via 3‐point bending testing. Independent t‐tests, effects sizes and percent differences were computed between the two groups (p < 0.05). The Trained Group had significantly larger normalized femur mass (p = 0.007) and normalized soleus muscle mass (p = 0.033) when compared to the Control Group. There was a medium or large effect size with the Trained Groups' femurs having larger mass, bone mineral density, rupture loads, cortical wall thickness, shaft cross sectional area, soleus mass, normalized gastrocnemius mass, and smaller shaft inner diameters compared to the Control Group. These changes may contribute to decreasing osteoporosis and fracture risk in those with spinal cord injuries.

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Review of Secondary Causes of Osteoporotic Fractures Due to Diabetes and Spinal Cord Injury

Cited by 6 publications

References 83 publications

Expression of lncRNA MALAT1 through miR-144-3p in Osteoporotic Tibial Fracture Rats and Its Effect on Osteogenic Differentiation of BMSC under Traction

Expression of lncRNA MALAT1 through miR-144-3p in Osteoporotic Tibial Fracture Rats and Its Effect on Osteogenic Differentiation of BMSC under Traction

Exploring caregivers’ perceptions of community-based service requirements of patients with spinal cord injury: a qualitative study

Effect of robotic gait training on muscle and bone characteristics in spinal cord transected rats

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