Review of Recommendations for Bioanalytical Method Validation: Chromatographic Assays and Ligand Binding Assays

Londhe, Vaishali; Rajadhyaksha, Madhura

doi:10.1007/s10337-018-3677-z

Cited by 1 publication

(1 citation statement)

References 42 publications

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“…Consequently, a glaring trend is that ligand binding assays are being increasingly replaced by LC-MS/MS (Shaaban & G orecki, 2015). For the study of substances in intricate biological matrices, LC-MS/MS offers outstanding selectivity, good accuracy and precision, and a wide dynamic range (Londhe & Rajadhyaksha, 2019;Pailleux & Beaudry, 2012). However, to date, no report has been published on simultaneous bioanalytical measurement of [ 19 F]-alfatide and alfatide using LC-MS/ MS.…”

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confidence: 99%

A novel LC–MS/MS approach to [¹⁹F]‐alfatide and alfatide for application in preclinical pharmacokinetics

Ren

Yuan

Shao

et al. 2023

Biomedical Chromatography

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For the further study of [19F]‐alfatide, the development of highly sensitive analytical methods for its determination is an urgent issue. In this paper, a method for simultaneously determining [19F]‐alfatide and alfatide using liquid chromatography/tandem mass spectrometry was created and validated. The plasma samples were pretreated using the protein precipitation method. Poroshell 120 EC‐C18 (4.6 × 50 mm, 2.7 μm, Agilent) was used for the separation of the analytes to be measured. The mobile phase, consisting of acetonitrile and water (0.1% acetic acid, 50:50, v/v), was delivered at a flow rate of 0.60 ml/min for sample analysis. Positive electrospray ionization was performed using multiple‐reaction monitoring with transitions of m/z 715.2 → 636.6 for [19F]‐alfatide, the ion pairs 700.3 → 851.5 for alfatide and the ion pairs 237.1 → 194.2 for carbamazepine (internal standard). According to the results, the method had high specificity, precision and accuracy as well as an extended linear range. The matrix effect and extraction recovery were also acceptable. The method was successfully applied to the pharmacokinetic study of [19F]‐alfatide in rats.

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Section: Introductionmentioning

confidence: 99%

A novel LC–MS/MS approach to [¹⁹F]‐alfatide and alfatide for application in preclinical pharmacokinetics

Ren

Yuan

Shao

et al. 2023

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

Review of Recommendations for Bioanalytical Method Validation: Chromatographic Assays and Ligand Binding Assays

Cited by 1 publication

References 42 publications

A novel LC–MS/MS approach to [¹⁹F]‐alfatide and alfatide for application in preclinical pharmacokinetics

A novel LC–MS/MS approach to [¹⁹F]‐alfatide and alfatide for application in preclinical pharmacokinetics

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Review of Recommendations for Bioanalytical Method Validation: Chromatographic Assays and Ligand Binding Assays

Cited by 1 publication

References 42 publications

A novel LC–MS/MS approach to [19F]‐alfatide and alfatide for application in preclinical pharmacokinetics

A novel LC–MS/MS approach to [19F]‐alfatide and alfatide for application in preclinical pharmacokinetics

Contact Info

Product

Resources

About

A novel LC–MS/MS approach to [¹⁹F]‐alfatide and alfatide for application in preclinical pharmacokinetics

A novel LC–MS/MS approach to [¹⁹F]‐alfatide and alfatide for application in preclinical pharmacokinetics