Review of pheromone receptors in heliothine species: expression, function, and evolution

Yang, Ke; Wang, Chen‐Zhu

doi:10.1111/eea.12982

Cited by 22 publications

(29 citation statements)

References 105 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…frugiperda ovipositor. Two SfruORs (SfruOR13 and SfruOR16) were clustered in the lepidopteran PR subfamily [ 23 , 24 ]. In addition, SfruOR13 and SfruOR16 were more abundantly expressed in male antennae than in female antennae, suggesting that these ORs are putative PRs that specifically function in sexual communication.…”

Section: Discussionmentioning

confidence: 99%

“…The number and protein sequences of ORs vary widely among insect orders [ 20 , 21 , 22 ]. Pheromone receptors (PRs), which represent a subclass of insect ORs, are proposed to be mainly involved in sex pheromone detections [ 23 , 24 ]. Members of the GR family, usually abundant in insect gustatory organs, detect nonvolatile compounds, including sugars, bitters, and plant secondary metabolites [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification and Characterization of Chemosensory Receptors in the Pheromone Gland-Ovipositor of Spodoptera frugiperda (J. E. Smith)

Sun

Dong

Yang

et al. 2022

Insects

View full text Add to dashboard Cite

Chemoreception by moth ovipositors has long been suggested, but underlying molecular mechanisms are mostly unknown. To reveal such chemosensory systems in the current study, we sequenced and assembled the pheromone gland-ovipositor (PG-OV) transcriptome of females of the fall armyworm, Spodoptera frugiperda, a pest of many crops. We annotated a total of 26 candidate chemosensory receptor genes, including 12 odorant receptors (ORs), 4 gustatory receptors (GRs), and 10 ionotropic receptors (IRs). The relatedness of these chemosensory receptors with those from other insect species was predicted by phylogenetic analyses, and specific genes, including pheromone receptors, ORco, CO2 receptors, sugar receptors, and IR co-receptors, were reported. Although real-time quantitative-PCR analyses of annotated genes revealed that OR and IR genes were mainly expressed in S. frugiperda antennae, two ORs and two IRs expressed in antennae were also highly expressed in the PG-OV. Similarly, GR genes were mainly expressed in the proboscis, but two were also highly expressed in the PG-OV. Our study provides the first large-scale description of chemosensory receptors in the PG-OV of S. frugiperda and provides a foundation for exploring the chemoreception mechanisms of PG-OV in S. frugiperda and in other moth species.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of Chemosensory Receptors in the Pheromone Gland-Ovipositor of Spodoptera frugiperda (J. E. Smith)

Sun

Dong

Yang

et al. 2022

Insects

View full text Add to dashboard Cite

show abstract

“…The OR tree was inferred with the ORs from D. abietella, Helicoverpa armigera, and O. furnacalis (Zhang T. et al, 2015;Guo et al, 2020). Of these, 35 HarmORs previously characterized were included (Liu et al, 2013;Jiang et al, 2014;Guo et al, 2020;Yang and Wang, 2021). The IRs from B. mori, D. abietella, H. armigera and O. furnacalis were used to infer the tree (Zhang T. et al, 2015;Liu et al, 2018;Xing et al, 2021).…”

Section: Phylogenetic Analysismentioning

confidence: 99%

Transcriptome Analysis and Characterization of Chemosensory Genes in the Forest Pest, Dioryctria abietella (Lepidoptera: Pyralidae)

Wang

Chun

et al. 2021

Front. Ecol. Evol.

View full text Add to dashboard Cite

In Lepidoptera, RNA sequencing has become a useful tool in identifying chemosensory genes from antennal transcriptomes, but little attention is paid to non-antennal tissues. Though the antennae are primarily responsible for olfaction, studies have found that a certain number of chemosensory genes are exclusively or highly expressed in the non-antennal tissues, such as proboscises, legs and abdomens. In this study, we report a global transcriptome of 16 tissues from Dioryctria abietella, including chemosensory and non-chemosensory tissues. Through Illumina sequencing, totally 952,658,466 clean reads were generated, summing to 142.90 gigabases of data. Based on the transcriptome, 235 chemosensory-related genes were identified, comprising 42 odorant binding proteins (OBPs), 23 chemosensory proteins (CSPs), 75 odorant receptors (ORs), 62 gustatory receptors (GRs), 30 ionotropic receptors (IRs), and 3 sensory neuron membrane proteins (SNMPs). Compared to a previous study in this species, 140 novel genes were found. A transcriptome-wide analysis combined with PCR results revealed that except for GRs, the majority of other five chemosensory gene families in Lepidoptera were expressed in the antennae, including 160 chemosensory genes in D. abietella. Using phylogenetic and expression profiling analyses, members of the six chemosensory gene repertoires were characterized, in which 11 DabiORs were candidates for detecting female sex pheromones in D. abietella, and DabiOR23 may be involved in the sensing of plant-derived phenylacetaldehyde. Intriguingly, more than half of the genes were detected in the proboscises, and one fourth of the genes were found to have the expression in the legs. Our study not only greatly extends and improves the description of chemosensory genes in D. abietella, but also identifies potential molecular targets involved in olfaction, gustation and non-chemosensory functions for control of this pest.

show abstract

“…The types of PRs expressed in the antennae of Heliothis / Helicoverpa species are very similar. By altering the expression levels and functions of the PRs, the pheromone perception system in the male decodes the changes in the composition and ratio of sex pheromones released by the female [ 24 , 28 , 29 , 65 , 66 ]. Previous studies have shown that cis - and trans -regulatory variants participate in the regulation of the expression divergence of olfactory-related protein genes in related insects [ 67 ].…”

Section: Discussionmentioning

confidence: 99%

The Genetic Basis of Gene Expression Divergence in Antennae of Two Closely Related Moth Species, Helicoverpa armigera and Helicoverpa assulta

Guo

Dong

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

The closely related species Helicoverpa armigera (H. armigera) and Helicoverpa assulta (H. assulta) have different host plant ranges and share two principal components of sex pheromones but with reversed ratios. The antennae are the main olfactory organ of insects and play a crucial role in host plant selection and mate seeking. However, the genetic basis for gene expression divergence in the antennae of the two species is unclear. We performed an allele-specific expression (ASE) analysis in the antennal transcriptomes of the two species and their F1 hybrids, examining the connection between gene expression divergence and phenotypic differences. The results show that the proportion of genes classified as all cis was higher than that of all trans in males and reversed in females. The contribution of regulatory patterns to gene expression divergence in males was less than that in females, which explained the functional differentiation of male and female antennae. Among the five groups of F1 hybrids, the fertile males from the cross of H. armigera female and H. assulta male had the lowest proportion of misexpressed genes, and the inferred regulatory patterns were more accurate. By using this group of F1 hybrids, we discovered that cis-related regulations play a crucial role in gene expression divergence of sex pheromone perception-related proteins. These results are helpful for understanding how specific changes in the gene expression of olfactory-related genes can contribute to rapid evolutionary changes in important olfactory traits in closely related moths.

show abstract

Review of pheromone receptors in heliothine species: expression, function, and evolution

Cited by 22 publications

References 105 publications

Identification and Characterization of Chemosensory Receptors in the Pheromone Gland-Ovipositor of Spodoptera frugiperda (J. E. Smith)

Identification and Characterization of Chemosensory Receptors in the Pheromone Gland-Ovipositor of Spodoptera frugiperda (J. E. Smith)

Transcriptome Analysis and Characterization of Chemosensory Genes in the Forest Pest, Dioryctria abietella (Lepidoptera: Pyralidae)

The Genetic Basis of Gene Expression Divergence in Antennae of Two Closely Related Moth Species, Helicoverpa armigera and Helicoverpa assulta

Contact Info

Product

Resources

About