Review of Osteosarcoma and Current Management

Durfee, Ryan A.; Mohammed, Maryam K.; Luu, Hue H.

doi:10.1007/s40744-016-0046-y

Cited by 313 publications

(314 citation statements)

References 129 publications

Supporting

Mentioning

308

Contrasting

Unclassified

Order By: Relevance

“…Combined with consequent surgical resection, neoadjuvant chemotherapy has become the standard treatment [4]. However, the accompanying drug resistance and side effects substantially weaken the therapeutic effectiveness [22].…”

Section: Discussionmentioning

confidence: 99%

“…A combinational application of neoadjuvant chemotherapy and consequent surgical resection significantly increased the five-year survival rate of patients without metastasis to approximately 60%-70% [3]. However, the outcome is disappointing in metastatic patients, with a five-year survival rate of only 5%-20% [4, 5]. Additionally, severe side effects and drug resistance are common.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Deoxyelephantopin Induces Reactive Oxygen Species-Mediated Apoptosis and Autophagy in Human Osteosarcoma Cells

Zou

Zhang

Sun

et al. 2017

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Osteosarcoma is the predominant form of primary bone malignancy. Although the combinational application of neoadjuvant chemotherapy and surgical resection significantly increases the survival rate, the therapeutic outcome remains unsatisfactory. Deoxyelephantopin (DET), an active ingredient of Elephantopus scaber, has been reported to have an anti-tumor effect in recent publications. This study aimed to investigate whether DET has antineoplastic effects on osteosarcoma cells and its underlying mechanism. Methods: Cell viability and morphological changes were assessed by MTT and Live/dead assays. Cell apoptosis, reactive oxygen species (ROS) and mitochondrial membrane potential were detected utilizing Annexin V-FITC/PI double staining, DCFH-DA and JC-1 probes, respectively. Autophagy was detected by mRFP-GFP-LC3 adenovirus transfection and western blot. Results: DET dose-dependently reduced the viability of osteosarcoma cells following the increase in intracellular ROS levels. Pretreatment with N-acetylcysteine (NAC) reversed this effect. Furthermore, DET induced mitochondrial apoptosis. Depolarized cells were increased, and apoptosis-related proteins, such as Bax, Bcl-2, cleaved caspase-9, cleaved caspase-3 and cleaved ploy ADP-ribose polymerase, were activated. Additionally, we found that DET could induce autophagy in osteosarcoma cells, but autophagy inhibition did not affect the decrease in cell viability. Conclusion: DET induced apoptosis in osteosarcoma cells through ROS generation, mitochondrial dysfunction and caspase activation; in addition, autophagy was involved in the effects of DET on osteosarcoma cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Deoxyelephantopin Induces Reactive Oxygen Species-Mediated Apoptosis and Autophagy in Human Osteosarcoma Cells

Zou

Zhang

Sun

et al. 2017

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Osteosarcoma is the eighth most common primary malignant skeletal tumor affecting children and adolescent. 1,2 Despite the emergence of neoadjuvant chemotherapy, the 5-year survival for osteosarcoma patients with metastasis or recurrence remains poor. 3 The poor prognosis is due to, at least in part, aggressive biologic behavior and delayed diagnosis.…”

Section: Introductionmentioning

confidence: 99%

CBX2 is a functional target of miRNA let‐7a and acts as a tumor promoter in osteosarcoma

Han

Cao

et al. 2019

Cancer Medicine

View full text Add to dashboard Cite

Osteosarcoma is the most common type of primary malignant tumor of skeletal with poor prognosis in children and adolescents. Accumulating evidence indicates that CBX2 is overexpressed in multiple human neoplasm and play a critical role in tumorigenesis and progression. However, its functional role and upstream regulation mechanism in osteosarcoma remain unknown. In the present study, tissue microarray (TMA) analysis was performed to determine the association between CBX2 expression and clinical prognosis of osteosarcoma patients by immunohistochemistry. We also investigated the functional role of CBX2 using small interfering RNA (siRNA) in vitro and in vivo. Additionally, we confirmed the direct binding between CBX2 and let‐7a via qPCR, western blot and luciferase reporter assay. We found that CBX2 is dramatically upregulated in osteosarcoma tissues and high CBX2 expression was correlated with metastasis, recurrence, and chemotherapy response, as well as unfavorable prognosis in patients with osteosarcoma. Similar results were observed in a sarcoma cohort from The Cancer Genome Atlas (TCGA) dataset. Further experiments revealed that CBX2 knockdown significantly impeded osteosarcoma cell proliferation and invasion ability in vitro, and suppressed the tumor growth in tumor xenografts model. Mechanistically, we confirmed that CBX2 is a functional target of miRNA let‐7a. Overexpression of let‐7a inhibits osteosarcoma cell proliferation, which was reversed by CBX2 overexpression. Taken together, our study demonstrates that let‐7a/CBX2 plays a crucial role in osteosarcoma progression. CBX2 could serve as a promising prognostic biomarker and potential therapeutic target for osteosarcoma patients.

show abstract

“…Currently the 5‐year overall survival rate is approximately 70% for patients diagnosed with primary non‐metastatic extremity OSA and ESA . However, the prognosis for patients with metastatic OSA or ESA at presentation is poor with a 5‐year event‐free survival (EFS) of less than 30% …”

Section: Introductionmentioning

confidence: 99%

Functional outcome after surgery in patients with bone sarcoma around the knee; results from a long‐term prospective study

Dam

Bekkering

Bramer

et al. 2017

Journal of Surgical Oncology

View full text Add to dashboard Cite

Background and ObjectivesIn a previous conducted study functional outcome of young patients with bone sarcoma located around the knee was longitudinally evaluated during the first 2 years postoperatively. Functional outcome improved significantly over the first 2 years. The purpose of this descriptive study was to evaluate the functional outcome of these patients at long‐term follow‐up of 7 years.MethodsFunctional outcome was assessed with the TESS, MSTS, Baecke questionnaire, and three functional performance tests: time up and down stairs (TUDS), various walking activities (VWA), and the 6‐min walking test (6MWT). Linear Mixed Model has been employed for the repeated measurements.ResultsTwenty patients of the original study (n = 44) participated in the current study. Fifteen limb‐salvage and five ablative surgery patients, median follow‐up 7.4 years (6.8‐8.0) (CI 95%), mean age 22.3 years (18.2‐31.6). Between 2 and 7 years after surgery, 8 limb‐salvage patients (53%) encountered surgery related complications. Questionnaires and functional performance tests showed no significant difference in functional outcome between 2 years and 7 years after surgery (P < 0.05).ConclusionsBetween 2 years and follow‐up at average 7 years after surgery no further improvements were noticed at young patients with a bone sarcoma located around the knee.

show abstract

Review of Osteosarcoma and Current Management

Cited by 313 publications

References 129 publications

Deoxyelephantopin Induces Reactive Oxygen Species-Mediated Apoptosis and Autophagy in Human Osteosarcoma Cells

Deoxyelephantopin Induces Reactive Oxygen Species-Mediated Apoptosis and Autophagy in Human Osteosarcoma Cells

CBX2 is a functional target of miRNA let‐7a and acts as a tumor promoter in osteosarcoma

Functional outcome after surgery in patients with bone sarcoma around the knee; results from a long‐term prospective study

Contact Info

Product

Resources

About