Review of Microinjection Systems

Xu, Qingsong

doi:10.1007/978-3-319-74621-0_2

Cited by 13 publications

(8 citation statements)

References 110 publications

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“…Most of the earlier microinjection strategies were manual, performed by bringing the biomolecule-loaded nanoneedles onto the cells. For adherent cells, cell-substrate provided the necessary reaction force to facilitate injection, while micropipettes were used to hold the suspended cells during the microinjection process [ 62 , 83 ]. Although these methods allowed the transduction of a vast extent of biomolecules into an extensive range of single cells with good controllability, high transduction rates, and cell viability, it is limited by the requirement of a skilled technician, expensive instrumentation, and lower throughput rates [ 62 , 82 , 83 ].…”

Section: Microfluidic Mechanoporationmentioning

confidence: 99%

“…For adherent cells, cell-substrate provided the necessary reaction force to facilitate injection, while micropipettes were used to hold the suspended cells during the microinjection process [ 62 , 83 ]. Although these methods allowed the transduction of a vast extent of biomolecules into an extensive range of single cells with good controllability, high transduction rates, and cell viability, it is limited by the requirement of a skilled technician, expensive instrumentation, and lower throughput rates [ 62 , 82 , 83 ]. Recent advances in microfluidic platform-based approaches for microinjection have reversed the microinjection strategy, incorporated automation [ 81 ] and/or robotics [ 84 ] in the process to obtain precise delivery and higher throughputs [ 62 , 82 , 83 ].…”

Section: Microfluidic Mechanoporationmentioning

confidence: 99%

“…The objective of this review, hence, is to briefly emphasize and provide an overview of different microfluidic-based mechanoporation techniques. The authors would like to mention that microinjection [ 62 , [81] , [82] , [83] , [84] ] and micro/nanoneedle arrays [ [85] , [86] , [87] , [88] , [89] , [90] , [91] , [92] , [93] ] are very broad fields in themselves, with a plethora of literature, which have been summarized in some detailed and dedicated reviews. The scope of this review will, however, only be to highlight some of the key developments in these areas with a focus on microfluidic-based techniques.…”

Section: Introductionmentioning

confidence: 99%

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Microfluidic mechanoporation for cellular delivery and analysis

Chakrabarty

Gupta

Illath

et al. 2022

Materials Today Bio

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Highly efficient intracellular delivery strategies are essential for developing therapeutic, diagnostic, biological, and various biomedical applications. The recent advancement of micro/nanotechnology has focused numerous researches towards developing microfluidic device-based strategies due to the associated high throughput delivery, cost-effectiveness, robustness, and biocompatible nature. The delivery strategies can be carrier-mediated or membrane disruption-based, where membrane disruption methods find popularity due to reduced toxicity, enhanced delivery efficiency, and cell viability. Among all of the membrane disruption techniques, the mechanoporation strategies are advantageous because of no external energy source required for membrane deformation, thereby achieving high delivery efficiencies and increased cell viability into different cell types with negligible toxicity. The past two decades have consequently seen a tremendous boost in mechanoporation-based research for intracellular delivery and cellular analysis. This article provides a brief review of the most recent developments on microfluidic-based mechanoporation strategies such as microinjection, nanoneedle arrays, cell-squeezing, and hydroporation techniques with their working principle, device fabrication, cellular delivery, and analysis. Moreover, a brief discussion of the different mechanoporation strategies integrated with other delivery methods has also been provided. Finally, the advantages, limitations, and future prospects of this technique are discussed compared to other intracellular delivery techniques.

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Section: Microfluidic Mechanoporationmentioning

confidence: 99%

Section: Microfluidic Mechanoporationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Microfluidic mechanoporation for cellular delivery and analysis

Chakrabarty

Gupta

Illath

et al. 2022

Materials Today Bio

View full text Add to dashboard Cite

show abstract

“…Injection involves the mechanical penetration of the plasma membrane with a needle-like probe through which molecules can enter the cell ( Figure 1A). Microinjection uses a conical micron-sized glass micropipette and a pressure controller, which controls the delivery of material into the cell ( Figure 1B) [16]. The positioning of the micropipette is controlled by a micromanipulator, which enables the injection of cells at a defined site.…”

Section: Injection Of Cells: From Microscale To Nanoscale Probesmentioning

confidence: 99%

Methods for protein delivery into cells: from current approaches to future perspectives

Chau

Actis

Hewitt

2020

Biochemical Society Transactions

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The manipulation of cultured mammalian cells by the delivery of exogenous macromolecules is one of the cornerstones of experimental cell biology. Although the transfection of cells with DNA expressions constructs that encode proteins is routine and simple to perform, the direct delivery of proteins into cells has many advantages. For example, proteins can be chemically modified, assembled into defined complexes and subject to biophysical analyses prior to their delivery into cells. Here, we review new approaches to the injection and electroporation of proteins into cultured cells. In particular, we focus on how recent developments in nanoscale injection probes and localized electroporation devices enable proteins to be delivered whilst minimizing cellular damage. Moreover, we discuss how nanopore sensing may ultimately enable the quantification of protein delivery at single-molecule resolution.

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“…However, improving the cost, safety, speed, throughput, and efficiency of nonviral transfection remains a challenge for the broader application of gene therapies to patient care. Of note, nanoparticle delivery, microinjection, electroporation, and lipofection are efficient techniques but vary in efficacy and throughput, depending on the cell line or the platform (11)(12)(13). Several clinical trials have shown that a minimum of 2 million cells/kg of body weight is needed for the effective engraftment of CD34 + -selected hematopoietic stem cell populations used for gene therapies (14).…”

mentioning

confidence: 99%

Acoustofluidic sonoporation for gene delivery to human hematopoietic stem and progenitor cells

Belling

Heidenreich

Tian

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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Advances in gene editing are leading to new medical interventions where patients’ own cells are used for stem cell therapies and immunotherapies. One of the key limitations to translating these treatments to the clinic is the need for scalable technologies for engineering cells efficiently and safely. Toward this goal, microfluidic strategies to induce membrane pores and permeability have emerged as promising techniques to deliver biomolecular cargo into cells. As these technologies continue to mature, there is a need to achieve efficient, safe, nontoxic, fast, and economical processing of clinically relevant cell types. We demonstrate an acoustofluidic sonoporation method to deliver plasmids to immortalized and primary human cell types, based on pore formation and permeabilization of cell membranes with acoustic waves. This acoustofluidic-mediated approach achieves fast and efficient intracellular delivery of an enhanced green fluorescent protein-expressing plasmid to cells at a scalable throughput of 200,000 cells/min in a single channel. Analyses of intracellular delivery and nuclear membrane rupture revealed mechanisms underlying acoustofluidic delivery and successful gene expression. Our studies show that acoustofluidic technologies are promising platforms for gene delivery and a useful tool for investigating membrane repair.

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Review of Microinjection Systems

Cited by 13 publications

References 110 publications

Microfluidic mechanoporation for cellular delivery and analysis

Microfluidic mechanoporation for cellular delivery and analysis

Methods for protein delivery into cells: from current approaches to future perspectives

Acoustofluidic sonoporation for gene delivery to human hematopoietic stem and progenitor cells

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