Review of micro-optical sectioning tomography (MOST): technology and applications for whole-brain optical imaging [Invited]

Zheng, Ting; Feng, Zuren; Wang, Xiaojun; Jiang, Tao; Jin, Rui; Zhao, Peilin; Luo, Ting; Gong, Hui; Luo, Qi; Yuan, Jing

doi:10.1364/boe.10.004075

Cited by 24 publications

(28 citation statements)

References 110 publications

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“…Optical methods have the potential for scalable large-area high-resolution mapping. For instance, cutting-edge microscopy techniques such as micro-optical sectioning tomography (MOST) 5 , 6 , serial two-photon tomography (STP) 7 , block-face serial microscopy (FAST) 8 , and light sheet microscopy (LSM) 9 , 10 , permit the reconstruction of large volumes of tissue and even entire organs with micrometer resolution. However, they need a source of contrast to detect the structure of interest.…”

Section: Introductionmentioning

confidence: 99%

Autofluorescence enhancement for label-free imaging of myelinated fibers in mammalian brains

Costantini

Baria

Sorelli

et al. 2021

Sci Rep

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Analyzing the structure of neuronal fibers with single axon resolution in large volumes is a challenge in connectomics. Different technologies try to address this goal; however, they are limited either by the ineffective labeling of the fibers or in the achievable resolution. The possibility of discriminating between different adjacent myelinated axons gives the opportunity of providing more information about the fiber composition and architecture within a specific area. Here, we propose MAGIC (Myelin Autofluorescence imaging by Glycerol Induced Contrast enhancement), a tissue preparation method to perform label-free fluorescence imaging of myelinated fibers that is user friendly and easy to handle. We exploit the high axial and radial resolution of two-photon fluorescence microscopy (TPFM) optical sectioning to decipher the mixture of various fiber orientations within the sample of interest. We demonstrate its broad applicability by performing mesoscopic reconstruction at a sub-micron resolution of mouse, rat, monkey, and human brain samples and by quantifying the different fiber organization in control and Reeler mouse's hippocampal sections. Our study provides a novel method for 3D label-free imaging of nerve fibers in fixed samples at high resolution, below micrometer level, that overcomes the limitation related to the myelinated axons exogenous labeling, improving the possibility of analyzing brain connectivity.

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Section: Introductionmentioning

confidence: 99%

Autofluorescence enhancement for label-free imaging of myelinated fibers in mammalian brains

Costantini

Baria

Sorelli

et al. 2021

Sci Rep

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“…The other method is to image the surface of the tissue and remove it layer by layer. For example, the recently developed micro‐optical sectioning tomography (MOST) [6], serial two‐photon tomography (STP) [7], and block‐face serial microscopy (FAST) [8] techniques all adopt this principle. Notably, the sectioning imaging method has a distinct insufficiency, as the tissue sample can be used only once after serial removal [4].…”

Section: Introductionmentioning

confidence: 99%

Organic solvent‐based tissue clearing techniques and their applications

Zhan

Liu

et al. 2021

Journal of Biophotonics

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Revealing the true structure of tissues and organs with tissue slicing technology is difficult since images reconstructed in three dimensions are easily distorted. To address the limitations in tissue slicing technology, tissue clearing has been invented and has recently achieved significant progress in three‐dimensional imaging. Currently, this technology can mainly be divided into two types: aqueous clearing methods and solvent‐based clearing methods. As one of the important parts of this technology, organic solvent‐based tissue clearing techniques have been widely applied because of their efficient clearing speed and high clearing intensity. This review introduces the primary organic solvent‐based tissue clearing techniques and their applications.

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“…Cleared samples can be imaged using techniques such as light-sheet microscopy (2,4,7) and optical projection tomography (OPT) (10,11). However, clearing requires often complex, lengthy and costly tissue preparation, with variable efficacy (9,12), and widely acknowledged morphological changes to the sample (9,13). Additionally, sample size is limited by the working distance of the microscope objective lens (7) and the objective lens must be protected from the often corrosive clearing solutions.…”

Section: Introductionmentioning

confidence: 99%

“…However, block-face imaging can suffer from loss of optical resolution in the z-axis, due to contamination by out of focus light from below the block surface (shine-through) (15). The addition of optical-sectioning capabilities such as two-photon and structured illumination into serial sectioning instruments has aimed to overcome this issue (3), (14), (16) (13,17), but at the cost of dramatically increasing the technical requirements for the imaging instrument require high powered lasers and often they are custom built.…”

Section: Introductionmentioning

confidence: 99%

“…This included the development of a MF-HREM tissue-processing, acquisition and post-processing pipeline, with the use of an opacifying agent and image deconvolution to recover axial image resolution. As MF-HREM does not need tissues to be optically cleared, it is advantageous for imaging tissue morphology (13) and can be used in conjunction with lipophilic dyes that cannot be used in solvent based clearing (26,27). For MF-HREM, lateral resolution is determined directly by the microscope objective, while axial resolution is related to both section thickness and light penetration into the block.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Multi-Fluorescence High-Resolution Episcopic Microscopy (MF-HREM) for Three-Dimensional Imaging of Adult Murine Organs

Walsh

Holroyd

Finnerty

et al. 2020

Preprint

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10 Three-dimensional microscopy of large biological samples (>0.5 cm 3 ) is transforming 11 biological research. Many existing techniques require trade-offs between image resolution 12 and sample size, require clearing or use optical sectioning. These factors complicate the 13 implementation of large volume 3D imaging. Here we present Multi-fluorescent High 14 Resolution Episcopic Microscopy (MF-HREM) which allows 3D imaging of large samples 15 without the need for clearing or optical sectioning. 16MF-HREM uses serial-sectioning and block-facing wide-field fluorescence, without the need 17 for tissue clearing or optical sectioning. We detail developments in sample processing 18 including stain penetration, resin embedding and imaging. In addition, we describe image 19 post-processing methods needed to segment and further quantify these data. Finally, we 20 demonstrate the wide applicability of MF-HREM by: 1) quantifying adult mouse glomeruli. 2) 21 identifying injected cells and vascular networks in tumour xenograft models; 3) quantifying 22 vascular networks and white matter track orientation in mouse brain.

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Review of micro-optical sectioning tomography (MOST): technology and applications for whole-brain optical imaging [Invited]

Cited by 24 publications

References 110 publications

Autofluorescence enhancement for label-free imaging of myelinated fibers in mammalian brains

Autofluorescence enhancement for label-free imaging of myelinated fibers in mammalian brains

Organic solvent‐based tissue clearing techniques and their applications

Multi-Fluorescence High-Resolution Episcopic Microscopy (MF-HREM) for Three-Dimensional Imaging of Adult Murine Organs

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