Review of major meat-borne zoonotic bacterial pathogens

Ali, Sultan; Alsayeqh, Abdullah F.

doi:10.3389/fpubh.2022.1045599

Cited by 26 publications

(22 citation statements)

References 98 publications

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“…These pathogens present a significant health risk to consumers, as they can cause a range of foodborne illnesses, varying in severity . Staphylococcus aureus can lead to skin infections, pneumonia, and bacteremia, while L. monocytogenes can result in meningitis, listeriosis, and complications during pregnancy ( Ali and Alsayeqh, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Bacteriological evaluation and advanced SYBR-green multiplex real-time PCR assay for detection of minced meat adulteration

Sheikh,

Whab,

ElDaly

et al. 2024

Open Vet J

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Background: Minced meat is a valuable source of nutrients, but it is vulnerable to contamination by microorganisms commonly present in the environment. Additionally, there is a risk of adulteration with cheaper meat sources, which can be harmful to consumers. Aim: It is crucial to identify meat adulteration with distinct microbiological analysis for legal, economic, religious, and public health purposes. Methods: A total of 100 minced meat samples were collected from several markets in Sharkia Governorate, Egypt. These samples were then subjected to bacteriological testing and an advanced multiplex PCR method. This method enables the detection of bovine, equine, porcine, and dog species in meat samples with just one step. Results: The adulterated samples had a higher total bacterial count and pH values compared to pure bovine meat. These differences in bacterial count and pH values were statistically significant, with p-values of 0.843 (log10) and 0.233, respectively. The frequency of E. coli occurrence was 13%, and the O111 serotype was predominant in the adulterated samples. Listeria monocytogenes and S. aureus were isolated with prevalence rates of 3% and 29%, respectively. Besides, the SYBR-green multiplex real-time PCR assay used in this study detected adulteration with dog, equine, and porcine meats in the examined samples at rates of 9%, 5%, and 4%, respectively. Conclusion: This method provides a sensitive and specific approach to detect issues related to well-being and safety.

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Section: Discussionmentioning

confidence: 99%

Bacteriological evaluation and advanced SYBR-green multiplex real-time PCR assay for detection of minced meat adulteration

Sheikh,

Whab,

ElDaly

et al. 2024

Open Vet J

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“…4 Ali and Alsayaqh stress that pathogenic microbes, spread through food consumption, can have a range of impacts, from mild (such as diarrhea) to fatal (such as cancerous conditions); the entirety of this range must be taken seriously as an issue of public health, especially in the interest of preventing the overloading of health care systems. 5 While further alignment of goals under the One Health framework is seen as a necessary response to zoonotic disease issues, biotechnological innovations continue to receive attention.…”

Section: Contextmentioning

confidence: 99%

Reduced zoonoses and improved nutritional profiles: interrogating cultured meat and its alleged health benefits

Giles

2023

Journal of Global Health Reports

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Cultured meat is being marketed as a multi-faceted improvement over traditional meat production. Some proponents claim that cultured meat reduces the potential spread of zoonotic disease; others further claim that cultured meat can be made more nutritious than traditional meat. This paper demonstrates – through a review of citations regarding proponent claims surrounding cultured meat’s potential to reduce zoonotic disease spread and improve nutritional possibilities – that cultured meat’s alleged health benefits are not based on quantitative data, nor based on well-developed theoretical research. Claims are often based on presumptions held throughout the literature; this paper calls these presumptions into question by investigating theoretical questions related to how cultured meat will be produced. Importantly, the paper also examines the recent emergence of “exotic cultured meat,” positing that proponent ambitions for diversified food experiences are not exempt from concerns about zoonotic disease spread and nutritional value. Healthcare professionals need to be aware of the limited evidence available for health-related claims which are being used to promote cultured meat. While such a conclusion does not require dismissing cultured meat’s potential, greater scrutiny is needed at this time, especially as cultured meat inches closer to becoming publicly available. This paper develops cultured meat research further by identifying the need for: deeper consideration of the interaction between humans and animals throughout the supply chain; greater care to be taken regarding the use of various sources as definitive proof of cultured meat’s alleged health benefits; and critical consideration of the implications of exotic cultured meat production.

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“…Globally, the growing demand for meat consumption and changing trends in production and supply in the past five decades pose as new challenges in food safety and public health concerns [1,2]. Meat of animal origins can potentially harbour a plethora of pathogens including bacteria, virus, and parasites leading to zoonotic diseases [2][3][4][5]. It is estimated that approximately millions of deaths and one billion cases of illness are in connection with zoonoses annually [4].…”

Section: Introductionmentioning

confidence: 99%

Quantification of Pork, Chicken, Beef, and Sheep Contents in Meat Products Using Duplex Real-Time PCR

Wang¹,

Teo²,

Wu³

et al. 2023

Foods

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Accurate methods for meat speciation and quantification are essential for ensuring the supply of safe and wholesome meat and composite products with animal origins to negate the potential associated hazards, aid classification of consignments at the import control system, and thwart food fraud committed for financial gain. To better enhance meat safety control and combat food fraud, this study developed two duplex real-time polymerase chain reaction (real-time PCR) systems specifically designed for chicken, pork, sheep, and beef, using single-copy, chromosomally encoded, species-specific gene sequences to accurately measure the content of each meat type in meat products. DNA extracted from the raw and boiled reference materials prepared in varying proportions (ranging from 1% to 75%) were used in the development of the duplex assay to derive calibration factors to determine the meat content in different meat products. The method was further validated using proficiency test samples and market monitoring samples. Our findings showed that this method exhibits high specificity and sensitivity, with a significant accuracy range of 0.14% to 24.07% in quantifying the four meat types in both raw and processed meat products. Validation results further confirmed the effectiveness of our method in accurately quantifying meat content. Thus, we have demonstrated the duplex qPCR assays as promising approaches for implementation in routine analysis to strengthen meat safety control systems and combat meat fraud, thereby safeguarding consumer health and trust in the meat industry.

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Review of major meat-borne zoonotic bacterial pathogens

Cited by 26 publications

References 98 publications

Bacteriological evaluation and advanced SYBR-green multiplex real-time PCR assay for detection of minced meat adulteration

Bacteriological evaluation and advanced SYBR-green multiplex real-time PCR assay for detection of minced meat adulteration

Reduced zoonoses and improved nutritional profiles: interrogating cultured meat and its alleged health benefits

Quantification of Pork, Chicken, Beef, and Sheep Contents in Meat Products Using Duplex Real-Time PCR

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