Review of Keratinocyte Culture Techniques: Problems of Growing Skin

Breidahl, A.F.; Juoson, R. T.; Clunie, G. J. A.

doi:10.1111/j.1445-2197.1989.tb01615.x

Cited by 28 publications

(19 citation statements)

References 48 publications

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“…Moreover, this method needs more time according to the reports by Breidahl et al (1989) and Kedjarune et al (2001). When compared with the enzymatic method, the absence of a feeder-layer supports cell differentiation well.…”

Section: Discussionmentioning

confidence: 90%

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Comparison of two cellular harvesting methods for primary human oral culture of keratinocytes

Klingbeil

Herson

Cristo³

et al. 2009

Cell Tissue Bank

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The possibility of obtaining transplantable oral epithelia opens new perspectives for oral treatments. Most of them are surgical, resulting in mucosal failures. As reconstructive material this in vitro epithelia would be also useful for other parts of the human body. Many researchers still use controversial methods; therefore it was evaluated and compared the efficiency of the enzymatic and direct explant methods to obtain oral keratinocytes. To this project oral epithelia fragments were used. This work compared: time needed for cell obtainment, best cell amount, life-span and epithelia forming cell capacity. The results showed the possibility to obtain keratinocytes from a small oral fragment and we could verify the advantages and peculiar restrictions. We concluded that under our conditions the enzymatic method showed the best results: in the cells obtaining time needed, cell amount and life-span. Both methods showed the same capacity to form in vitro epithelia.

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Section: Discussionmentioning

confidence: 90%

“…Other authors like Breidahl et al (1989), differ on the method preference. Freshney (2000), highlights that the direct explant method should be chosen when the tissue sample is short.…”

Section: Discussionmentioning

confidence: 96%

Comparison of two cellular harvesting methods for primary human oral culture of keratinocytes

Klingbeil

Herson

Cristo³

et al. 2009

Cell Tissue Bank

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“…Cholera toxin, epidermal growth factor and hydrocortisone As these threeadditivies have almost universally been demonstrated to be of benefit in other species (9). their effects in rabbit disaggregated keratinocyte culture were examined together.…”

Section: Fibronectin Feeder Layermentioning

confidence: 99%

“…A multitude of variables in culture technique have been described for keratinocyte cell culture in species other than the rabbit (9). These variables may be categorized into the groups of skin preparation, feeder layers, nutrients and growth hormones.…”

Section: Introductionmentioning

confidence: 99%

In vitro culture of disaggregated rabbit keratinocytes

Breidahl

Judson

Dumble

et al. 1990

Immunology & Cell Biology

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Summary A icchnique Is described for the culture of disaggregated rabbit keratinocytes and the produclion of confluenl sheets of immunologically pure keratinocytes suitable for use in transplantalion studies. The benefits of various feeder layers, nutrients and growth hormones were examined by the use of paired controls. The ability of the cultured keratinocylc sheets to be removed from the culture vessel intact and survive as viable autografts was demonstrated, thus establishing a new animal model for the investigation of allograft rejection responses to cultured keratinocylcs. The benefits of using the rabbit mode! for such studies and the advantages of this culture technique over a previously described method of rabbit kcratinocyte cell culture are discussed.

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“…Diese beiden Bestandteile stellen -insbesondere beim Einsatz bei chirurgischen Wahleingriffen -Risiken dar, da hierdurch bei der Transplantation Fremd-DNA und Fremdprotein ª tragen werden k6nnen [1]. So wurde bei Patienten, die mit fetalem K~ilber-serum gezª Epithettransplantate erhalten harten, von Meyer et al [4] die Bildung von Antik6rpem gegen bovines Serumalbumin nachgewiesen.…”

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Modifikationen in der Kultivierung von Gingivakeratinozyten

Lauer

Otten

Schilli

1997

Mund Kiefer GesichtsChir

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Gingival keratinocyte grafts are usually cultured using 3T3 mouse fibroblasts as feeder cells and fetal calf serum as growth factor. These additives entail risks due to xenogenic DNA and protein. Therefore the explant and the disperse culture technique free of feeder cells were compared, and autogenous human serum was tested. Twelve halved gingival biopsies were trypsinized and cultured as single-cell suspensions, the other halves were cultured as explants. Six halved biopsies were cultured in autogenous serum, the other halves in fetal calf serum. Growth, morphology and cell biological aspects were compared. The single-cell suspensions did not form a confluent epithelial layer, whereas all explants formed confluent primary gingival keratinocyte cultures. The keratinocytes' growth in autogenous serum was equivalent to that in fetal calf serum. Morphology and cytokeratin expression were identical. The explant technique combined with autogenous serum can be used for culturing gingival autografts as well as for individual cultures for special issues.

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Review of Keratinocyte Culture Techniques: Problems of Growing Skin

Cited by 28 publications

References 48 publications

Comparison of two cellular harvesting methods for primary human oral culture of keratinocytes

Comparison of two cellular harvesting methods for primary human oral culture of keratinocytes

In vitro culture of disaggregated rabbit keratinocytes

Modifikationen in der Kultivierung von Gingivakeratinozyten

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