Review of in situ derivatization techniques for enhanced bioanalysis using liquid chromatography with mass spectrometry

Baghdady, Yehia Z.; Schug, Kevin A.

doi:10.1002/jssc.201501003

Cited by 42 publications

(24 citation statements)

References 72 publications

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“…This topic is given a considerable coverage in the literature, with dedicated books, reviews (e.g. Baghdady & Schug, ; Santa, , ), papers and technical applications covering this topic.…”

Section: Derivatization In Bioanalysismentioning

confidence: 99%

Sample preparation for large‐scale bioanalytical studies based on liquid chromatographic techniques

Medvedovici

Bacalum

David

2017

Biomedical Chromatography

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Quality of the analytical data obtained for large-scale and long term bioanalytical studies based on liquid chromatography depends on a number of experimental factors including the choice of sample preparation method. This review discusses this tedious part of bioanalytical studies, applied to large-scale samples and using liquid chromatography coupled with different detector types as core analytical technique. The main sample preparation methods included in this paper are protein precipitation, liquid-liquid extraction, solid-phase extraction, derivatization and their versions. They are discussed by analytical performances, fields of applications, advantages and disadvantages. The cited literature covers mainly the analytical achievements during the last decade, although several previous papers became more valuable in time and they are included in this review.

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“…This topic is given a considerable coverage in the literature, with dedicated books, reviews (e.g. Baghdady & Schug, ; Santa, , ), papers and technical applications covering this topic.…”

Section: Derivatization In Bioanalysismentioning

confidence: 99%

Sample preparation for large‐scale bioanalytical studies based on liquid chromatographic techniques

Medvedovici

Bacalum

David

2017

Biomedical Chromatography

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“…In order to improve the detection sensitivity and separation selectivity, AAs are often derivatized [13,15]. The recent review by Baghdady and Schug clearly presents the commonly used in situ derivatization techniques coupled to liquid chromatography with mass spectrometry-based bioanalysis [16].…”

Section: D/l Amino Acid Ratio As a Tool For The Determination Of Age mentioning

confidence: 99%

Enantiomeric Ratio of Amino Acids as a Tool for Determination of Aging and Disease Diagnostics by Chromatographic Measurement

2016

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Abstract:Occurrence of D-amino acids in living organisms is a useful indicator of various changes, diseases, or disorders. Determination of amino acid enantiomers, namely the enantiomeric ratio of amino acids or excess of certain D-amino acids, represents a useful tool in the studies of aging processes or biomarkers in disease/disorder diagnosis in humans. The amount of D-amino acids is usually very low. Therefore, suitable sample pretreatment, often derivatization, and highly selective and sensitive separation methods are essential for D-amino acid analysis in this field. Chromatographic techniques offer appropriate choices for solving these tasks. This review covers the advances in methodology and development of improved instrumental chromatographic methods focused on D,L-amino acid separation and determination. New findings in the area of possible D-amino acid biomarkers are also included.

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“…Several reviews are available on thiols analysis. When thiols are present in the trace, specific detectors such as AED or PFPD are preferred to classic FID and simple quadrupole MS detectors. Purification can increase thiols concentration and thus detection .…”

Section: Introductionmentioning

confidence: 99%

Characterization of odour‐active compounds in Timur (Zanthoxylum armatum DC.) fruits from Nepal

Cicchetti¹,

Duroure²,

Pérez³

et al. 2017

Flavour & Fragrance J

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Essential oils and CO2 extracts of Timur (Zanthoxylum armatum DC syn. Zanthoxylum alatum Hemsl.) from fruits cropped in the Nepalese districts Surkhet, Jajarkot and Salyan from 2012 to 2015 were analysed to explain their typical odour and characterize their volatile profile. Best extraction conditions to ensure the right balance of the terpenic fresh notes compared with the sulphury fruity character were obtained with milled fruits and CO2 extraction. Volatile composition allowed supplying zones discrimination. Among more than 130 volatile constituents, identified by GC–MS and quantified by GC‐FID with the use of predicted relative response factors, only 20 were found to have an odour impact by GC‐O using a hybrid methodology between detection frequency and time intensity methods. Limonene, α‐pinene and myrcene explain the terpenic notes. Linalool, citronellal, 6‐methyl heptanal and octanal bring the fatty and aldehydic tone. The spicy facet is linked to (E)‐methyl cinnamate and (Z)‐ethyl cinnamate. 4‐mercapto‐4‐methylpentan‐2‐one, 3‐mercaptohexanol, 3‐mercaptohexyl acetate and 1‐p‐menthene‐8‐thiol are the key olfactive constituents contributing to the typical tropical and grapefruit odours. Their presence was confirmed using the stable isotope dilution assay and reported for the first time in Timur.

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Review of in situ derivatization techniques for enhanced bioanalysis using liquid chromatography with mass spectrometry

Cited by 42 publications

References 72 publications

Sample preparation for large‐scale bioanalytical studies based on liquid chromatographic techniques

Sample preparation for large‐scale bioanalytical studies based on liquid chromatographic techniques

Enantiomeric Ratio of Amino Acids as a Tool for Determination of Aging and Disease Diagnostics by Chromatographic Measurement

Characterization of odour‐active compounds in Timur (Zanthoxylum armatum DC.) fruits from Nepal

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