Review of gel permeation chromatography

Lambert, A.

doi:10.1002/pi.4980030103

Cited by 17 publications

(3 citation statements)

References 161 publications

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“…GPC is a method that allows for the separation, purification and analysis of a mixture of substances based on their molecular size [353]. It allows for the molecular weight and the molecular weight distribution of a polymer sample to be determined [354].…”

Section: Gel Permeation Chromatography (Gpc)mentioning

confidence: 99%

Development of METx: A Vasopressin Agonist

Patel¹

2016

Preprint

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Vasopressin (AVP) was one of the first peptides to be extracted and produced synthetically in the early 1950s. Since then AVP and its analogues have been increasingly researched as both AVP agonists and antagonists have therapeutic uses. Although much time and effort has been used to design potent selective AVP agonists and antagonists, very few have reached the market. AVP agonist analogues have only been approved for diabetes insipidus and variceal bleeding. Current main stay treatment for diabetes insipidus is desmopressin, a synthetic analogue of AVP. Desmopressin is well tolerated by the majority of patients, however severe adverse affects, such as hyponatraemia and water intoxication require a strict fluid intake, restricting fluid intake. Our aim is to develop METx for the treatment of diabetes insipidus to help overcome some of the limitations in lifestyle. The solubility and aggregation of METx was measured using the shake flask and pyrene assay respectively. The activity of METx was determined by measuring cAMP production by MDCK cells. The activity of METx was compared to that AVP, the natural agonist of the vasopressin receptor. Formulation studies were performed using GCPQ to create a formulation that could be used subcutaneously or orally. The stability of GCPQ formulations was determined in plasma, simulated gastric fluid (SGF) and rat intestinal wash (RIW). The activity of METx was measured in vivo by monitoring the urine production of rats upon administration of METx intravenously. METx was practically insoluble in aqueous solvents such as water and PBS, with increased solubility in organic solvents. Using TEM imaging aggregation of METx of was seen, the pyrene assay determined the critical micelle concentration to be 27.4mg l-1. METx was found to be a partial agonist of the vasopressin 2 receptor naturally expressed on the MDCK cells, it exhibited a similar EC 50 to AVP. Formulating with GCPQ a ratio of 1:5 (METx:GCPQ) was found to be the most stable in plasma, SGF and RIW. In vivo studies found METx to have a dose dependent reduction in urine production in rats, with 40mg kg-1 rats not producing any urine. The experiments suggest the development of a novel partial agonist of the vasopressin 2 receptor in vitro. The addition of GCPQ results in the formation of nanoparticles, with a ratio of 1:5 (METx:GCPQ) most stable in plasma, SGF and RIW. In vivo experiments resulted in reduced urine production by rats, in a dose responsive manner.

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Section: Gel Permeation Chromatography (Gpc)mentioning

confidence: 99%

Development of METx: A Vasopressin Agonist

Patel¹

2016

Preprint

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“…A general review of gel filtration chromatography (188) and chromatography on Sephadex H-20 (305) have been published. General aspects of gel permeation chromatography (123,135,206,207,789,1059,1466,1585), theory (1516), terms and relations (124), calibration of GPC columns (254), and polymer characterization by GPC (76) have been reviewed.…”

Section: Column Adsorption and Partition Chromatographymentioning

confidence: 99%

“…Gamma-aminobutyric acid has been determined quantitatively by one-dimensional paper chromatography (807), while urinary ß-aminoisobutyric acid has been separated from phenylalanine and tyrosine (1291). Urinary tryptophan metabolites have been studied by thin-layer chromatography on silica gel plates (789).…”

Section: Neutral Diol Plasmologens Have Been Synthesizedmentioning

confidence: 99%