Review of Fluorescence Spectroscopy in Environmental Quality Applications

Zacharioudaki, Despoina-Eleni; Fitilis, Ioannis; Kotti, Melina

doi:10.3390/molecules27154801

Cited by 30 publications

(20 citation statements)

References 114 publications

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“…To examine the ability of a given spectrometer and collection optics to detect UV emission, mercury calibration lamps are useful sources of bright, sharp peaks in the UV and visible region with well-defined emission wavelengths. , These can be found as small, low-powered, and portable sources for easy calibration of bench-top spectrometer systems. Strong lines are present across the UV-A, UV-B, and UV-C range, and detection of these lines is positive confirmation that a spectrometer system is appropriately capable of UV-light detection.…”

Section: Results and Discussionmentioning

confidence: 99%

UV Emission from Lanthanide-Doped Upconversion Nanoparticles in Super-Resolution Microscopy: Potential for Cellular Damage

Karami

Prinse

Spooner

et al. 2023

ACS Appl. Nano Mater.

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Upconversion nanoparticles (UCNPs) co-doped with lanthanide ions have recently attracted significant attention as fluorescent probes for super-resolution microscopy (SRM). This is due to the advantages of UCNPs over other fluorescence probes, such as fluorescent proteins, owing to their unique optical properties, limited photobleaching, and sharp emissions. However, the concurrent emission of ultraviolet (UV) wavelength radiation by UCNPs and the potential for cell photodamage, which may limit useful live-cell analysis, have been overlooked. Here, UCNPs synthesized with eight commonly used combinations of Yb/Tm and Yb/Tm/Gd dopants were excited by either pulsed- or continuous-wave (CW) lasers to evaluate their UV emission. The ratio of emitted UV-A and UV-B was measured relative to blue emission at 475 nm, which is traditionally used for imaging during SRM. We demonstrate that most UCNP samples emit UV light and that the dopant concentration has a key role in generating UV emissions. In addition, the use of pulsed or CW lasers for excitation can lead to a large variation in the amount of UV emitted. This work highlights the importance of considering upconversion dopant composition and concentration, as well as analyzing the emission of synthesized UCNPs before their use to prevent unwanted cell photodamage during live-cell imaging by SRM. Moreover, it established a need to improve the visible light emission of UCNPs with respect to UV emission for SRM applications.

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Section: Results and Discussionmentioning

confidence: 99%

UV Emission from Lanthanide-Doped Upconversion Nanoparticles in Super-Resolution Microscopy: Potential for Cellular Damage

Karami

Prinse

Spooner

et al. 2023

ACS Appl. Nano Mater.

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“…The two types of photoluminescence are fluorescence and phosphorescence. Fluorescence occurs when an electron from a singlet excited level radiatively decays to the ground singlet state within a typical decay period of 10 −10 s to 10 −7 s. In phosphorescence, on the other hand, the electron molecules return to their ground state through a triplet state and take a much longer time [ 36 ].…”

Section: Methodsmentioning

confidence: 99%

Optical Characterization of Biological Tissues Based on Fluorescence, Absorption, and Scattering Properties

2022

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Optical diagnostics methods are significantly appealing in biological applications since they are non-destructive, safe, and minimally invasive. Laser-induced fluorescence is a promising optical spectrochemical analytical technique widely employed for tissue classification through molecular analysis of the studied samples after excitation with appropriate short-wavelength laser light. On the other hand, diffuse optics techniques are used for tissue monitoring and differentiation based on their absorption and scattering characteristics in the red to the near-infrared spectra. Therefore, it is strongly foreseen to obtain promising results by combining these techniques. In the present work, tissues under different conditions (hydrated/dry skin and native/boiled adipose fat) were distinguished according to their fluorescence emission, absorption, and scattering properties. The selected tissues’ optical absorption and scattering parameters were determined via Kubelka–Munk mathematical model according to the experimental tissue reflectance and transmittance measurements. Such measurements were obtained using an optical configuration of integrating sphere and spectrometer at different laser wavelengths (808, 830, and 980 nm). Moreover, the diffusion equation was solved for the fluence rate at the sample surface using the finite element method. Furthermore, the accuracy of the obtained spectroscopic measurements was evaluated using partial least squares regression statistical analysis with 0.87 and 0.89 R-squared values for skin and adipose fat, respectively.

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“…Commonly used organic pollutant indicators, such as chemical oxygen demand (COD) and total organic carbon (TOC), can only provide an overall indication of the organic matter content and are unable to accurately reflect the composition and concentration of various dissolved organic substances in wastewater. Fluorescence spectroscopy, however, is better suited to reflect the properties, type, and quantity of organic matter in water due to its high selectivity and sensitivity; fluorescence spectroscopy can be employed for qualitative analysis, quantitative evaluation, and real-time online monitoring of wastewater treatment effects. , Marhuenda-Egea et al have demonstrated that three-dimensional fluorescence spectroscopy can be utilized to analyze and evaluate soluble organic matter. Xi-Zhi Niu et al employed three-dimensional fluorescence spectroscopy to investigate the soluble organic matter in black soil.…”

Section: Introductionmentioning

confidence: 99%

Adsorption and Degradation of Organics in Wastewater on Municipal Sludge

Ji,

Zhu,

et al. 2023

ACS Omega

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Adsorption and degradation of organic compounds on sludge were investigated by comparing activated and inactivated sludge at various dosages, pH values, and temperatures. The organic compounds in wastewater were identified and evaluated through fluorescence spectra. The results show that optimum adsorption occurred as the activated and inactivated sludge concentration was 4000 mg/L at a pH of 7.99 and a temperature of 30 °C. The fluorescence scanning spectrum indicated that activated sludge could remove protein-like organic matter, fulvic acid-like organic matter, and humic acid-like organic matter by 22.1, 9.4, and 41.2%, respectively, via adsorption only or by 25.9, 9.8, and 74.3%, respectively, via adsorption and degradation. Under optimum conditions, by using the good adsorption performance of sludge combined with other sewage treatment technologies, the treatment of high-content organic wastewater can be achieved.

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Review of Fluorescence Spectroscopy in Environmental Quality Applications

Cited by 30 publications

References 114 publications

UV Emission from Lanthanide-Doped Upconversion Nanoparticles in Super-Resolution Microscopy: Potential for Cellular Damage

UV Emission from Lanthanide-Doped Upconversion Nanoparticles in Super-Resolution Microscopy: Potential for Cellular Damage

Optical Characterization of Biological Tissues Based on Fluorescence, Absorption, and Scattering Properties

Adsorption and Degradation of Organics in Wastewater on Municipal Sludge

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