Background and Objective: The G 0 ʻMedianʼ potato was designed to adapt in medium-altitude land and to produce seed tuber instead of food material. This study aimed to evaluate the growth and production of G 0 ʻMedianʼ potato under different media composition and fertilizer application methods in medium-altitude land. Materials and Methods: Two weeks old post acclimatization plantlet of G 0 ʻMedianʼ potato was prepared as plant material and then arranged in a split-plot design with two factors (growing media and chemical fertilizer application method) and three replications. Results: The result showed that the less proportion of soil in growing media could improve the field capacity, porosity, air space and water holding capacity, resulting in more loose structures suitable for potatoes (GM 1 >GM 2 >GM 3 ). The interaction of GM 1 +F 2 produced the significantly heaviest plant biomass (dry weight of plant and root). However, the best treatment was GM 2 +F 2 with the highest number of tuber per plant, i.e., 6 tuber per plant. Conclusion: High frequency (10x) of diluted granular fertilizer by using watering method (F 2 ) was suitable to improve not only the number of stolon and tuber but also the quality of harvested tuber as indicated by the lower percentage of small seed tuber.