Review of 4Pi Fluorescence Nanoscopy

Hao, Xiang; Li, Yiming; Fu, Shuang; Li, Yanghui; Xu, Yingke; Kuang, Cuifang; Liu, Xü

doi:10.1016/j.eng.2020.07.028

Cited by 13 publications

(5 citation statements)

References 84 publications

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“…Combining multi-color sequential staining 43 , 45 , 53 with iPALM imaging, would be a promising approach to recovering single loop structure without the use of statistical modeling. Interestingly, recently new imaging technologies such as Minflux 66 , SIMflux 67 , or 4pi-SMLM 68 were reported. They aim at improving the localization precision of the probes and the resolution of the images while limiting the effects of fixation on the biological samples.…”

Section: Discussionmentioning

confidence: 99%

Super-resolution visualization of chromatin loop folding in human lymphoblastoid cells using interferometric photoactivated localization microscopy

Parteka-Tojek

Zhu

Lee

et al. 2022

Sci Rep

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The three-dimensional (3D) genome structure plays a fundamental role in gene regulation and cellular functions. Recent studies in 3D genomics inferred the very basic functional chromatin folding structures known as chromatin loops, the long-range chromatin interactions that are mediated by protein factors and dynamically extruded by cohesin. We combined the use of FISH staining of a very short (33 kb) chromatin fragment, interferometric photoactivated localization microscopy (iPALM), and traveling salesman problem-based heuristic loop reconstruction algorithm from an image of the one of the strongest CTCF-mediated chromatin loops in human lymphoblastoid cells. In total, we have generated thirteen good quality images of the target chromatin region with 2–22 nm oligo probe localization precision. We visualized the shape of the single chromatin loops with unprecedented genomic resolution which allowed us to study the structural heterogeneity of chromatin looping. We were able to compare the physical distance maps from all reconstructed image-driven computational models with contact frequencies observed by ChIA-PET and Hi-C genomic-driven methods to examine the concordance between single cell imaging and population based genomic data.

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Section: Discussionmentioning

confidence: 99%

Super-resolution visualization of chromatin loop folding in human lymphoblastoid cells using interferometric photoactivated localization microscopy

Parteka-Tojek

Zhu

Lee

et al. 2022

Sci Rep

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“…4Pi-SMLM uses two opposing objectives to coherently detect the single molecule fluorescence and achieved sub-10 nm isotropic 3D resolution even for single molecules with 250 photons collected by each objective( 26 ). In 4Pi-SMLM, the change of axial position of the emitter will change the optical path length difference (OPD) within the interference cavity, thus changing the interference phase of the self-interfered individual photons.…”

Section: Resultsmentioning

confidence: 99%

Fast and universal single molecule localization using multi-dimensional point spread functions

Li,

Shi,

Liu

et al. 2023

Preprint

Self Cite

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The recent development of single molecule imaging techniques has enabled not only high accuracy spatial resolution imaging but also information rich functional imaging. Abundant information of the single molecules can be encoded in its diffraction pattern and be extracted precisely (e.g. 3D position, wavelength, dipole orientation). However, sophisticated high dimensional point spread function (PSF) modeling and analyzing methods have greatly impeded the broad accessibility of these techniques. Here, we present a graphics processing unit (GPU)-based B-spline PSF modeling method which could flexibly model high dimensional PSFs with arbitrary shape without greatly increasing the model parameters. Our B-spline fitter achieves 100 times speed improvement and minimal uncertainty for each dimension, enabling efficient high dimensional single molecule analysis. We demonstrated, both in simulations and experiments, the universality and flexibility of our B-spline fitter to accurately extract the abundant information from different types of high dimensional single molecule data including multicolor PSF (3D + color), multi-channel four-dimensional 4Pi-PSF (3D + interference phase) and five-dimensional vortex PSF (3D + dipole orientation).

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“…However, limited axial resolution compared to the improved lateral resolution remains a hindrance, particularly when studying complex three-dimensional arrangements within biological specimens 3,4 . While techniques using dual objective lenses such as 4PI microscopy and I 5 M microscopy have shown promise in enhancing axial resolution, they often pose challenges due to their complex and resource-intensive set-ups [5][6][7] . Recently, approaches like 4-beam structured illumination microscopy (SIM) have emerged, which employ mirrors alongside a single objective lens in 3D SIM, effectively confining axial excitation volume and contributing to optical sectioning for improved resolution 8,9 .…”

Section: Introductionmentioning

confidence: 99%

Nanoscale fluctuation-enhanced axial localization microscopy integrated with metallic structure-assisted fluidic chips

Yoo,

Ko,

et al. 2024

Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XXI

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Optical super-resolution microscopy has revolutionized imaging in the lateral axis, enabling nanoscale structure visualization with unprecedented detail. However, achieving high axial resolution along the z-axis remains challenging. In this study, nanoscale fluctuation-enhanced axial localization microscopy addresses this issue by incorporating metallic structures, specifically reflective optical devices, into dynamic speckle illumination microscopy. By controlling light waves within the fluidic chip, these metallic devices enable super-resolution to be achieved not only in the lateral direction but also along the z-axis, all in a cost-effective manner. Experimental investigation using 100-nm fluorescent beads and the U-87 MG cell membrane demonstrates axial-resolving performance of fluctuation-enhanced imaging compared to conventional methods. The application of an optical fluctuation-based reconstruction algorithm further allows the extraction of 4-fold enhanced axial information over diffraction-limited system.

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Review of 4Pi Fluorescence Nanoscopy

Cited by 13 publications

References 84 publications

Super-resolution visualization of chromatin loop folding in human lymphoblastoid cells using interferometric photoactivated localization microscopy

Super-resolution visualization of chromatin loop folding in human lymphoblastoid cells using interferometric photoactivated localization microscopy

Fast and universal single molecule localization using multi-dimensional point spread functions

Nanoscale fluctuation-enhanced axial localization microscopy integrated with metallic structure-assisted fluidic chips

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