Review of 3D cell culture with analysis in microfluidic systems

Castiaux, Andre D; Spence, Dana M.; Martin, R. Scott

doi:10.1039/c9ay01328h

Cited by 96 publications

(77 citation statements)

References 103 publications

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“…Castiaux et al (2019a) demonstrate two novel techniques to 3DP enclosed microfluidic channels via a PolyJet 3D printer. The first way implements a liquid to support cover layer prints while the second method uses a polycarbonate membrane to support the additional layers (Castiaux et al, 2019b). Shimizu et al (2019) designed an ECM collagen-based stretchable microfluidic system that resembles the in vivo blood vessels and allows for in vitro 3D cell culture.…”

Section: Dp Microfluidic Devices For 3d Cell Culturementioning

confidence: 99%

Is It Time to Start Transitioning From 2D to 3D Cell Culture?

Jensen

Teng

2020

Front. Mol. Biosci.

1,004

802

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Section: Dp Microfluidic Devices For 3d Cell Culturementioning

confidence: 99%

Is It Time to Start Transitioning From 2D to 3D Cell Culture?

Jensen

Teng

2020

Front. Mol. Biosci.

1,004

802

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“…Suspension-based and scaffold-based (fiber or paper) cultures are compatible with microfluidic devices offering different analytical approaches. 83 OOCs are useful for disease and organ modeling. Although investing in these devices the first time around is relatively costly as compared to devices required for other techniques, the longevity and stability of the device makes up for the expenditure.…”

Section: Choice Of Culture System and Assaymentioning

confidence: 99%

Devices and techniques used to obtain and analyze three‐dimensional cell cultures

Agrawal

Ramesh

Aishwarya

et al. 2021

Biotechnology Progress

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Cell cultures are indispensable for both basic and applied research. Advancements in cell culture and analysis increase their utility for basic research and translational applications. A marked development in this direction is advent of three-dimensional (3D) cultures. The extent of advancement in 3D cell culture methods over the past decade has warranted referring to a single cell type being cultured as an aggregate or spheroid using simple scaffolds as "traditional." In recent years, the development of "next-generation" devices has enabled cultured cells to mimic their natural environments much better than the traditional 3D culture systems. Automated platforms like chip-based devices, magnetic-and acoustics-based assembly devices, dielectrophoresis (DEP), micro pocket cultures (MPoC), and 3D bio-printing provide a dynamic environment compared to the rather static conditions of the traditional simple scaffold-based 3D cultures. Chip-based technologies, which are centered on prin-

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“…The microscale manipulation of colloidal macromolecule suspensions is of high significance in view of the use of microfluidics and 3D ink-jet printing to obtain hydrogels with superior properties and on-demand functionalities [ 1 , 2 ]. These hydrogels find applications in analytical, biotechnology, and medical field as drug delivery systems [ 3 ], cell scaffolds [ 4 ], tissue engineering supports [ 5 ], and matrices for biosensors [ 6 ]. Moreover, regularly sized gel beads are receiving interest as possible components of liquid system displays or other sophisticated futuristic materials [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Process Conditions and Colloidal Properties of Cellulose Nanocrystals Suspensions on the Production of Hydrogel Beads

et al. 2021

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The influence of the physical, rheological, and process parameters on the cellulose nanocrystal (CNC) drops before and after external gelation in a CaCl2 solution was investigated. The dominant role of the CNC’s colloidal suspension properties, such as the viscous force, inertial, and surface tension forces in the fluid dynamics was quantitatively evaluated in the formation of drops and jellified beads. The similarity and difference between the behavior of carbohydrate polymers and rod-like crystallites such as CNC were enlightened. Pump-driven and centrifugally-driven external gelation approaches were followed to obtain CNC hydrogel beads with tunable size and regular shape. A superior morphological control—that is, a more regular shape and smaller dimension of the beads—were obtained by centrifugal force-driven gelation. These results suggest that even by using a simple set-up and a low-speed centrifuge device, the extrusion of a colloidal solution through a small nozzle under a centrifugal field is an efficient approach for the production of CNC hydrogel beads with good reproducibility, control over the bead morphology and size monodispersion.

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Review of 3D cell culture with analysis in microfluidic systems

Abstract: A review with 105 references that analyzes the emerging research area of 3D cell culture in microfluidic platforms with integrated detection schemes.

Cited by 96 publications

References 103 publications

Is It Time to Start Transitioning From 2D to 3D Cell Culture?

Is It Time to Start Transitioning From 2D to 3D Cell Culture?

Devices and techniques used to obtain and analyze three‐dimensional cell cultures

Effect of Process Conditions and Colloidal Properties of Cellulose Nanocrystals Suspensions on the Production of Hydrogel Beads

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