Review: Innate immunity, lipid metabolism and nutrition in canine atopic dermatitis

Pucheu‐Haston, Cherie M.; Santoro, Domenico; Bizikova, Petra; Eisenschenk, Melissa; Marsella, Rosanna; Nuttall, Tim

doi:10.1111/vde.12199

Cited by 25 publications

(25 citation statements)

References 127 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, the canine ATMs contain birefringent granules, which are electrodense when observed by transmission electron microscopy. Birefringent granulation is generally considered a hallmark of mast cells in dogs in which mast cells play a more prominent role in inflammatory and tissue healing processes than in other mammals (Dewald et al 2004;Pucheu-Haston et al 2015). This calls for caution when canine adipose tissue samples are analyzed by using acid toluidine blue, a standard staining method for mast cells, since ATMs are positively stained.…”

Section: Discussionmentioning

confidence: 99%

Adipose tissue macrophages in non-rodent mammals: a comparative study

et al. 2015

View full text Add to dashboard Cite

The stromal vascular fraction (SVF) of adipose tissue in rodents and primates contains mesenchymal stem cells and immune cells. SVF cells have complex metabolic, immune and endocrine functions with biomedical impact. However, in other mammals, the amount of data on SVF stem cells is negligible and whether the SVF hosts immune cells is unknown. In this study, we show that the SVF is rich in immune cells, with a dominance of adipose tissue macrophages (ATMs) in cattle (Bos primigenius taurus), domestic goat (Capra aegagrus hircus), domestic sheep (Ovis aries), domestic cat (Felis catus) and domestic dog (Canis familiaris). ATMs of these species are granulated lysosome-rich cells with lamellipodial protrusions and express the lysosome markers acid phosphatase 5 (ACP-5) and Mac-3/Lamp-2. Using ACP-5 and Mac-3/Lamp-2 as markers, we additionally detected ATMs in other species, such as the domestic horse (Equus ferus caballus), wild boar (Sus scrofa) and red fox (Vulpes vulpes). Feline and canine ATMs also express the murine macrophage marker F4/80 antigen. In the lean condition, the alternative macrophage activation marker CD206 is expressed by feline and canine ATMs and arginase-1 by feline ATMs. Obesity is associated with interleukin-6 and interferon gamma expression and with overt tyrosine nitration in both feline and canine ATMs. This resembles the obesity-induced phenotype switch of murine and human ATMs. Thus, we show, for the first time, that the presence of ATMs is a general trait of mammals. The interaction between the adipose cells and SVF immune cells might be evolutionarily conserved among mammals.

show abstract

Section: Discussionmentioning

confidence: 99%

Adipose tissue macrophages in non-rodent mammals: a comparative study

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Atopic dermatitis (AD) is a common disease of dogs and has been defined as pruritus and inflammation through immunoglobin E (IgE) production that predominately targets environmental antigens [1]. While the pathogenesis of AD is not completely understood, researchers have recently uncovered that AD involves the innate immune system and alterations in the epidermal barrier [2]. Traditional models of AD focused on genetic alterations, resulting in abnormal immune responses to typically harmless antigens.…”

Section: Introductionmentioning

confidence: 99%

Non-controlled, open-label clinical trial to assess the effectiveness of a dietetic food on pruritus and dermatologic scoring in atopic dogs

et al. 2019

View full text Add to dashboard Cite

Background Canine atopic dermatitis (AD) is a common skin disease. The goal of this study was to evaluate food designed to improve skin barrier function and lower inflammation to reduce pruritus and clinical severity in client-owned atopic dogs. The food contained an antioxidant blend to reduce oxidative stress, plant polyphenols to stabilize mast cells, and polyunsaturated fatty acids to improve skin health and reduce inflammation. Results Seventeen dogs were included in the analysis. Initially 48 adult atopic dogs were enrolled and exclusively fed a dermatologic food for 8 weeks in a non-controlled, open-label study. Thirty-one dogs were excluded for the following reasons: oral and topical medication changes ( n = 17), missing data ( n = 4), fatty acid supplementation ( n = 3), food refusal ( n = 3), dropped out ( n = 3), and owner concerns ( n = 1). Using a scale from 0 (normal) - 4 (severe), veterinarians evaluated the presence and severity of clinical signs of atopy at weeks 0, 4, and 8. Pet owners also rated their pet’s clinical signs of atopy on a scale from 0 (not present) - 10 (present continuously) at weeks 0, 4, and 8. Compared with initial baseline scores (median 19, range 3–69), the total veterinarian scores were significantly lower at weeks 4 (median 11, range 1–15) and 8 (median 7, range 3–46) ( p < 0.05). Similarly, owner assessments showed significant improvements in the least squares mean (LSM) from baseline to 4 weeks (itching, redness, licking, and scratching) continuing to 8 weeks (itching, redness, and scratching) ( p < 0.05). Conclusions In this open, non-controlled study evaluating a dermatologic diet in seventeen client-owned dogs, owner and veterinarian assessments showed statistically significant reductions in clinical scores designed to measure severity of atopic dermatitis. While these results show promise for the management of canine atopic dermatitis, controlled clinical trials are also needed to affirm our findings. Electronic supplementary material The online version of this article (10.1186/s12917-019-1929-2) contains supplementary material, which is available to authorized users.

show abstract

“…The underlying pathogenesis is a complex interplay of environmental factors, alterations of the innate and adaptive immune system and skin barrier dysfunction, and remains not fully understood. [7][8][9] In the majority of cases the allergy is directed against environmental and/or food antigens 10 and associated with specific T-cell subsets and cytokines. 7,11,12 As commercially available allergen tests cannot differentiate canine allergic dermatitis (flea, food or environmental allergies) from other skin diseases, their use is not recommended as screening tests.…”

Section: Introductionmentioning

confidence: 99%

Cutaneous microRNA expression in healthy Labrador and Golden retrievers and retrievers with allergic and inflammatory skin diseases

Morlang¹,

Weber²,

Bomhard³

et al. 2021

Veterinary Dermatology

View full text Add to dashboard Cite

Background -MicroRNAs (miRNA) are short, single-stranded RNA molecules that regulate gene expression in a post-transcriptional manner. Their expression is proposed to be tissue-specific and alterations in miRNA expression have been detected in many diseases.Objective -To compare miRNA expression in the skin of healthy Labrador and golden retrievers, and those with allergic and nonallergic dermatitis.Methods and materials -Formalin-fixed and paraffin-embedded (FFPE) skin specimens from seven healthy Labrador and golden retrievers, and seven dogs with allergic skin disease were collected. A further mixed nonallergic inflammation group consisted of samples from five dogs with fungal infection, demodicosis and mast cell tumours. Total RNA was extracted and miRNA primer assays for 18 target miRNAs (miR-142, miR-363, miR-18b, miR-451, miR-146a, miR-124, miR-409, miR-193b, miR-223, miR-215, miR-155, miR-423a, miR-143, miR-1839, miR-21, miR-34b, miR-146b and miR-202) were performed, with RNU6-2 and SNORD95 as miRNAs for normalisation. The selection of miRNAs for investigation was based on reported data and a pilot study evaluating miRNA extraction from FFPE tissue specimens.Results -In the two dogs with mast cell tumours, miRNA expression was undetermined for most miRNAs, so both were excluded from analysis. Although there were differences in the miRNA expression between healthy and inflamed skin, allergic and nonallergic inflammation showed similar expression patterns. Conclusion and clinical relevance -Although the number of included dogs was small, based on this study, none of the evaluated miRNAs allowed differentiation of allergic dermatitis from other inflammatory skin diseases in retriever dogs.

show abstract

Review: Innate immunity, lipid metabolism and nutrition in canine atopic dermatitis

Cited by 25 publications

References 127 publications

Adipose tissue macrophages in non-rodent mammals: a comparative study

Adipose tissue macrophages in non-rodent mammals: a comparative study

Non-controlled, open-label clinical trial to assess the effectiveness of a dietetic food on pruritus and dermatologic scoring in atopic dogs

Cutaneous microRNA expression in healthy Labrador and Golden retrievers and retrievers with allergic and inflammatory skin diseases

Contact Info

Product

Resources

About