Review Genomic in situ hybridization in plants

Silva, G.S.; Souza, Margarete Magalhães

doi:10.4238/2013.august.12.11

Cited by 48 publications

(41 citation statements)

References 49 publications

(45 reference statements)

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“…To date, the hypothesis on the hybrid origin of this passion fruit has not been tested in the field karyotype, and the probable parental genomes remain unclear. Among the tools of molecular cytogenetics, genomic in situ hybridization (GISH) is the most suitable tool to verify its origin and its probable parents (Silva & Souza 2013).…”

Section: Introductionmentioning

confidence: 99%

“…The GISH technique is efficient for distinguishing genomes and is used for the identification of natural and artificial hybrids, or to verify the genomic relationships between different species. This is because it predominantly detects the repetitive DNA sequences that compose most of the plant genome (Silva & Souza 2013; Silva et al . 2018).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Origin of the cultivated passion fruit Passiflora edulis f. flavicarpa and genomic relationships among species of the subgenera Decaloba and Passiflora

Silva

Souza

2020

Plant Biol J

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Passiflora edulis f. flavicarpa is the most economically important species in the genus Passiflora. However, the origin of this yellow form of passion fruit remains unclear, being suggested as a hybrid (P. edulis f. edulis × P. ligularis) or wild mutant. Here, the origin and genomic relationships of P. edulis f. flavicarpa with some related species in the genus Passiflora (subgenera Decaloba and Passiflora) were investigated using genomic in situ hybridization (GISH). Genomic DNA of 18 species was used as probe, which was hybridized onto chromosomes of P. edulis f. flavicarpa. Of all genomic DNA probes tested, none allowed us to identify a specific chromosome set in P. edulis f. flavicarpa. Conversely, probes from the subgenus Passiflora, P. edulis f. edulis, P. alata, P. cincinnata, P. coccinea, P. nitida and P. vitifolia, produced intense and uniform hybridizations on all chromosomes of P. edulis f. flavicarpa. Moreover, probes from P. ligularis, P. foetida and P. sublanceolata produced more intense hybridizations in the terminal region of four chromosomes, corresponding to the DNAr 45S locus, and also dispersed, less intense, hybridization across all chromosomes. Probes from the subgenus Decaloba, P. biflora, P. capsularis, P. cervii, P. coriacea, P. micropetala, P. morifolia, P. rubra and P. suberosa, produced hybridizations restricted to the DNAr 45S sites. The hybrid origin of P. edulis f. flavicarpa could not be supported based on the GISH results, and it is suggested that this species is conspecific with P. edulis f. edulis, because the probe with DNA of this form hybridized strongly throughout the target genome. The other putative parent species, P. ligularis, showed only a distant relationship with the target genome. The results also suggest that species of the subgenus Passiflora share many repetitive sequences and that the relationship between subgenera Decaloba and Passiflora is very distant.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Origin of the cultivated passion fruit Passiflora edulis f. flavicarpa and genomic relationships among species of the subgenera Decaloba and Passiflora

Silva

Souza

2020

Plant Biol J

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“…To differentiate the chromosomes of different genomes in polyploid individuals, the total genomic DNA of one parent is labeled and used as a probe, while a higher amount of unlabeled genomic DNA of the other parent is used to block the common repetitive sequences between the parents and to increase the specificity of DNA hybridisation. This technique is known as genomic in situ hybridisation (GISH) and can be used for studying intergenomic translocations and alien introgressions, and for discriminating genomes in polyploid cereals (Schwarzacher et al, 1989, 1992; Schubert et al, 2001; Silva and Souza, 2013). …”

Section: Techniques For Studying Genome Constitutions Of Pentaploid Wmentioning

confidence: 99%

Pentaploid Wheat Hybrids: Applications, Characterisation, and Challenges

et al. 2017

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Interspecific hybridisation between hexaploid and tetraploid wheat species leads to the development of F1 pentaploid hybrids with unique chromosomal constitutions. Pentaploid hybrids derived from bread wheat (Triticum aestivum L.) and durum wheat (Triticum turgidum spp. durum Desf.) crosses can improve the genetic background of either parent by transferring traits of interest. The genetic variability derived from bread and durum wheat and transferred into pentaploid hybrids has the potential to improve disease resistance, abiotic tolerance, and grain quality, and to enhance agronomic characters. Nonetheless, pentaploid wheat hybrids have not been fully exploited in breeding programs aimed at improving crops. There are several potential barriers for efficient pentaploid wheat production, such as low pollen compatibility, poor seed set, failed seedling establishment, and frequent sterility in F1 hybrids. However, most of the barriers can be overcome by careful selection of the parental genotypes and by employing the higher ploidy level genotype as the maternal parent. In this review, we summarize the current research on pentaploid wheat hybrids and analyze the advantages and pitfalls of current methods used to assess pentaploid-derived lines. Furthermore, we discuss current and potential applications in commercial breeding programs and future directions for research into pentaploid wheat.

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“…First, investigations of the genomic composition of all members of the tribe are required. To this end, genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH) are powerful cytogenetic techniques (Schwarzacher, 2003;Silva & Souza, 2013). These methods have been useful to elucidate the genomic composition of many members of the tribe (e.g., Elymus s.s., Ørgaard & Anamthawat-J onsson, 2001;Yang et al, 2016Yang et al, , 2017Liu et al, 2017;Hordelymus (Jess.)…”

Section: Introductionmentioning

confidence: 99%

Discovery of the genus Pseudoroegneria (Triticeae, Poaceae) in the Western Mediterranean on exploring the generic boundaries of Elymus

Lucía

Martínez‐Ortega

Rico

et al. 2018

J of Sytematics Evolution

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In this study, we review the classification of two species, Elymus hispanicus and E. marginatus, which are restricted to highly valuable and sensitive Mediterranean ecosystems. The genomic composition of the two species is analysed by in situ hybridization. In addition, lodicule morphology and foliar anatomy of both species are compared with those of E. caninus, E. repens, E. sibiricus (i.e., the type species of Elymus s.s.) and Pseudoroegneria strigosa (i.e., the type species of Pseudoroegneria). The genomic formula 2n = 8x = 56; HStStSt is proposed for E. hispanicus and 2n = 4x = 28; StSt for E. marginatus. In this latter species, the absence of the ribosomal genes in one of the two St genomes suggests that diploidization may have occurred during the evolution of the species. Regarding foliar anatomy, E. hispanicus, E. caninus, E. repens, and E. sibiricus shared several characteristics, but the leaf blades of E. marginatus proved anatomically more similar to those of Ps. strigosa. The data compiled support the contention that: (i) E. hispanicus belongs to Elymus s.s.; (ii) E. marginatus should be transferred to Pseudoroegneria; and (iii) the morphology of the lodicules should be carefully reconsidered for appropriately describing the boundaries between Elymus s.s. and Pseudoroegneria. The new combination Ps. marginata is proposed and a detailed iconography of the plant is provided.

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Review Genomic in situ hybridization in plants

Cited by 48 publications

References 49 publications

Origin of the cultivated passion fruit Passiflora edulis f. flavicarpa and genomic relationships among species of the subgenera Decaloba and Passiflora

Origin of the cultivated passion fruit Passiflora edulis f. flavicarpa and genomic relationships among species of the subgenera Decaloba and Passiflora

Pentaploid Wheat Hybrids: Applications, Characterisation, and Challenges

Discovery of the genus Pseudoroegneria (Triticeae, Poaceae) in the Western Mediterranean on exploring the generic boundaries of Elymus

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