Review. Fluorescence Microscopy Studies of Porous Silica Materials

Rühle, Bastian; Davies, Melari; Bein, Thomas; Bräuchle, Christoph

doi:10.5560/znb.2013-3068

Cited by 10 publications

(11 citation statements)

References 84 publications

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“…Single molecule fluorescence microscopy and tracking can be used to obtain valuable information about the porous system and the host‐guest interactions of nanomaterials . The dynamics of guests inside porous nanoparticles, and the interactions of the guests with the walls of the nanoparticles are sometimes the key for the targeted application.…”

Section: Characterization Methodsmentioning

confidence: 99%

Nanoparticle Characterization: What to Measure?

et al. 2019

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What to measure? is a key question in nanoscience, and it is not straightforward to address as different physicochemical properties define a nanoparticle sample. Most prominent among these properties are size, shape, surface charge, and porosity. Today researchers have an unprecedented variety of measurement techniques at their disposal to assign precise numerical values to those parameters. However, methods based on different physical principles probe different aspects, not only of the particles themselves, but also of their preparation history and their environment at the time of measurement. Understanding these connections can be of great value for interpreting characterization results and ultimately controlling the nanoparticle structure–function relationship. Here, the current techniques that enable the precise measurement of these fundamental nanoparticle properties are presented and their practical advantages and disadvantages are discussed. Some recommendations of how the physicochemical parameters of nanoparticles should be investigated and how to fully characterize these properties in different environments according to the intended nanoparticle use are proposed. The intention is to improve comparability of nanoparticle properties and performance to ensure the successful transfer of scientific knowledge to industrial real‐world applications.

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Section: Characterization Methodsmentioning

confidence: 99%

Nanoparticle Characterization: What to Measure?

et al. 2019

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“…(27, 63, 239, 240) There is also an interest in applications of single-molecule methods to bottom-up studies in polymer and materials science. (241–243) Here, we present selected studies that demonstrate the utility of accurate and precise 3D single-molecule localization for quantitative structural and dynamical measurements.…”

Section: Selected Applicationsmentioning

confidence: 99%

“…The development of single-molecule imaging techniques and their subsequent extension to three dimensions have benefited many domains of cell biology. ,,, There is also an interest in applications of single-molecule methods to bottom-up studies in polymer and materials science. − Here, we present selected studies that demonstrate the utility of accurate and precise 3D single-molecule localization for quantitative structural and dynamical measurements.…”

Section: Selected Applicationsmentioning

confidence: 99%

Three-Dimensional Localization of Single Molecules for Super-Resolution Imaging and Single-Particle Tracking

2017

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Single-molecule super-resolution fluorescence microscopy and single-particle tracking are two imaging modalities that illuminate the properties of cells and materials on spatial scales down to tens of nanometers, or with dynamical information about nanoscale particle motion in the millisecond range, respectively. These methods generally use wide-field microscopes and two-dimensional camera detectors to localize molecules to much higher precision than the diffraction limit. Given the limited total photons available from each single-molecule label, both modalities require careful mathematical analysis and image processing. Much more information can be obtained about the system under study by extending to three-dimensional (3D) single-molecule localization: without this capability, visualization of structures or motions extending in the axial direction can easily be missed or confused, compromising scientific understanding. A variety of methods for obtaining both 3D super-resolution images and 3D tracking information have been devised, each with their own strengths and weaknesses. These include imaging of multiple focal planes, point-spread-function engineering, and interferometric detection. These methods may be compared based on their ability to provide accurate and precise position information of single-molecule emitters with limited photons. To successfully apply and further develop these methods, it is essential to consider many practical concerns, including the effects of optical aberrations, field-dependence in the imaging system, fluorophore labeling density, and registration between different color channels. Selected examples of 3D super-resolution imaging and tracking are described for illustration from a variety of biological contexts and with a variety of methods, demonstrating the power of 3D localization for understanding complex systems.

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“…[413][414][415] The connectivity among mesochannels has been visualized by single-molecule fluorescence microscopy (see Ref. [416] for a review). In these experiments, luminescent probe molecules were incorporated in as-synthesized mesoporous silica, and their diffusion along the channels was followed with a fluorescence microscope.…”

Section: The Inner Surfaces Of Mesoporous Silica Materialsmentioning

confidence: 99%

Supramolecular Organization in Confined Nanospaces

Tabacchi

2018

ChemPhysChem

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Empty spaces are abhorred by nature, which immediately rushes in to fill the void. Humans have learnt pretty well how to make ordered empty nanocontainers, and to get useful products out of them. When such an order is imparted to molecules, new properties may appear, often yielding advanced applications. This review illustrates how the organized void space inherently present in various materials: zeolites, clathrates, mesoporous silica/organosilica, and metal organic frameworks (MOF), for example, can be exploited to create confined, organized, and self-assembled supramolecular structures of low dimensionality. Features of the confining matrices relevant to organization are presented with special focus on molecular-level aspects. Selected examples of confined supramolecular assemblies - from small molecules to quantum dots or luminescent species - are aimed to show the complexity and potential of this approach. Natural confinement (minerals) and hyperconfinement (high pressure) provide further opportunities to understand and master the atomistic-level interactions governing supramolecular organization under nanospace restrictions.

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Review. Fluorescence Microscopy Studies of Porous Silica Materials

Cited by 10 publications

References 84 publications

Nanoparticle Characterization: What to Measure?

Nanoparticle Characterization: What to Measure?

Three-Dimensional Localization of Single Molecules for Super-Resolution Imaging and Single-Particle Tracking

Supramolecular Organization in Confined Nanospaces

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