Review Degradation of microbial polyesters

Tokiwa, Yutaka; Calabia, Buenaventurada P.

doi:10.1023/b:bile.0000036599.15302.e5

Cited by 292 publications

(131 citation statements)

References 48 publications

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“…the degree of crystallinity, of the polymer. There are published data suggesting that depolymerizing enzymes preferentially attack amorphous regions of polymers rather than their ordered phases [8,30,31]. Assuming these observations are accurate, as the polymer is degraded and its amorphous phase is more rapidly disintegrated, the polymer that remains must exhibit an increase in its degree of crystallinity.…”

Section: Resultsmentioning

confidence: 99%

Degradable Polyhydroxyalkanoates as Herbicide Carriers

et al. 2012

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The biodegradable polymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) has been used to design experimental sustained-release formulations of the herbicide Zellek Super in the form of films and microgranules. The kinetics of polymer degradation and the dynamics of herbicide release show that the rate and extent of herbicide release from the polymer matrix into the soil depends on the geometry of the carrier and the proportion of the pesticide loaded into it (polymer/pesticide mass ratio). Experiments with the creeping bentgrass (Agrostis stolonifera L.) show that the formulations of the herbicide Zellek Super constructed as microgranules and films can be successfully used to suppress the growth of grasses. This study is the first to demonstrate that biodegradable polyhydroxyalkanoates can be used effectively to construct environmentally friendly sustained-release PHA-herbicide systems that can be placed into the soil together with seeds.

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Section: Resultsmentioning

confidence: 99%

Degradable Polyhydroxyalkanoates as Herbicide Carriers

et al. 2012

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“…Another reason that supports the theory that the lipase enzyme may share a similar mechanism in substrate hydrolysis with PHA depolymerase is that lipase also have a common amino acid sequence around the active side, Gly-X 1 -Ser-X 2 -Gly, which is also observed in PHA depolymerase. The only difference is that the X 1 residue in PHA depolymerase is substituted with histidine in lipase (Tokiwa & Calabia, 2004). In this study, lipase from SigmaAldrich, extracted from porcine pancreas was used in the form of lyophilized powder with an activity of ≥20,000 units/mg protein.…”

Section: In Vitro Degradation Of Electrospun P(3hb-co-4hb) Film In LImentioning

confidence: 99%

Fabrication and Degradation of Electrospun Polyhydroxyalkanoate Film

Lee¹,

Nanthini²,

Amirul³

et al. 2015

J. Sib. Fed. Univ., Biol.

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Polyhydroxyalkanoates (PHAs) are a family of biopolymers with good biodegradability. [P(3HB)], poly (3-hydroxybutyrate-co-25 mol % 4-hydroxybutyrate) ] and poly (3-hydroxybutyrate-co-75 mol % 4-hydroxybutyrate) (5, 10, 15 and 20 min) and concentrations (1 %, 2 %, 3 % and 4 %) P(3HB-co-75 mol % 4HB) films showed significant decrease in weight compared to P(3HB-co-25 mol % 4HB). Degraded P(3HB) had fibril-like structures whereas P(3HB-co-4HB) surface structure became more porous. Environmental degradation of these polymers was successful with P(3HB-co- 4HB) being better degraded.Keywords : polyhydroxyalkanoates, biopolymers, electrospinning, in vitro, degradation. DOI: 10.17516/1997: polyhydroxyalkanoates, biopolymers, electrospinning, in vitro, degradation. DOI: 10.17516/ -1389: polyhydroxyalkanoates, biopolymers, electrospinning, in vitro, degradation. DOI: 10.17516/ -2015 Ключевые слова : полигидроксиалканоаты, биополимеры, электростатическое формование, in vitro, деградация. Полигидроксиалканоаты (ПГА) представляют собой семейство биополимеров c хорошей биоразлагаемостью. С использованием электростатического формования были получены пленки из волокон поли-3-гидроксибутирата [П(3ГБ)], поли-3-гидроксибутирата-co-4-гидроксибутирата с содержанием 25 молярных % 4ГБ [П(3ГБ-co-25 мол. % 4ГБ)] и поли-3-гидроксибутирата-co-4-гидроксибутирата с содержанием 75 мол. % 4-гидроксибутирата [П(3ГБ-co-75 мол. % 4ГБ)]. Электростатическое формование П(3ГБ) после

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“…Most of the PHBV degrading microorganisms grow better at either ambient or mesophilic temperatures, whereas, only a few species like Bacillus strain TT96 (Tansengco and Tokiwa, 1998) and Streptomyces strain MG (Tokiwa and Calabia, 2004) were reported to degrade it at higher temperatures. There is little information on microbial degradation of PHBV at high temperatures (Takeda et al, 1998).…”

Section: Optimisation Of Culture Conditions For Phbv Depolymerase Promentioning

confidence: 99%

Isolation and characterisation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) degrading actinomycetes and purification of PHBV depolymerase from newly isolatedStreptoverticillium kashmirense AF1

et al. 2007

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Streptoverticillium kashmirense AF1 with the ability to degrade a natural polymer, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was isolated from municipal sewage sludge by soil burial technique. The PHBV film was degraded by the action of extracellular enzymes secreted by the microorganisms. Degradation of PHBV was evident by the formation of clear zones of hydrolysis on the polymer containing mineral salt agar plates. The extent of PHBV degradation increased up to 30 days of incubation. Maximum production of PHBV depolymerase was observed both at pH 8 and pH 7, 45 o C, 1% substrate concentration and in the presence of lactose as an additional carbon source. Two types of extracellular PHBV depolymerases were purified from S. kashmirense AF1 by gel permeation chromatography using Sephadex G-75. The molecular weights of the two proteins were found to be 35 and 45 kDa approximately, as determined by SDS-PAGE. The results of the Sturm test also showed more CO 2 production as a result of PHBV degradation, in the test as compared to control. The present findings indicated the degradation capabilities of S. kashmirense AF1.

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Review Degradation of microbial polyesters

Cited by 292 publications

References 48 publications

Degradable Polyhydroxyalkanoates as Herbicide Carriers

Degradable Polyhydroxyalkanoates as Herbicide Carriers

Fabrication and Degradation of Electrospun Polyhydroxyalkanoate Film

Isolation and characterisation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) degrading actinomycetes and purification of PHBV depolymerase from newly isolatedStreptoverticillium kashmirense AF1

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