Review: assessment of cell proliferation in histological material.

Hall, Peter A.; Levison, David A.

doi:10.1136/jcp.43.3.184

Cited by 414 publications

(198 citation statements)

References 146 publications

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“…In a variety of malignant neoplasms, significant correlations have been found between proliferative activity and metastatic potential, recurrence or overall prognosis (Hall and Levison, 1990). In our systematic review with meta-analysis, patients with Ki-67 positive tumours had shorter survival than those with Ki-67 negative tumours.…”

Section: Discussionmentioning

confidence: 63%

Ki-67 expression and patients survival in lung cancer: systematic review of the literature with meta-analysis

et al. 2004

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Among new biological markers that could become useful prognostic factors for lung carcinoma, Ki-67 is a nuclear protein involved in cell proliferation regulation. Some studies have suggested an association between Ki-67 and poor survival in lung cancer patients. In order to clarify this point, we have performed a systematic review of the literature, using the methodology already described by our Group, the European Lung Cancer Working Party. In total, 37 studies, including 3983 patients, were found to be eligible. In total, 49% of the patients were considered as having a tumour positive for the expression of Ki-67 according to the authors cutoff. In all, 29 of the studies dealt with non-small-cell lung carcinoma (NSCLC), one with small-cell carcinoma (SCLC), two with carcinoid tumours and five with any histology. In terms of survival results, Ki-67 was a bad prognosis factor for survival in 15 studies while it was not in 22. As there was no statistical difference in quality scores between the significant and nonsignificant studies evaluable for the meta-analysis, we were allowed to aggregate the survival results. The combined hazard ratio for NSCLC, calculated using a random-effects model was 1.56 (95% CI: 1.30 -1.87), showing a worse survival when Ki-67 expression is increased. In conclusion, our meta-analysis shows that the expression of Ki-67 is a factor of poor prognosis for survival in NSCLC.

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Section: Discussionmentioning

confidence: 63%

Ki-67 expression and patients survival in lung cancer: systematic review of the literature with meta-analysis

et al. 2004

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“…Various methods, including thymidine labelling indices, have been used to measure cell proliferation (Hall and Levison, 1990).…”

Section: Discussionmentioning

confidence: 99%

Cell proliferation, cell loss and expression of bcl-2 and p53 in human pulmonary neoplasms

Kennedy

Lamb²,

King³

et al. 1997

Br J Cancer

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Summary Immunohistochemical staining of bcl-2 and p53 proteins was compared with thymidine labelling index (TLI) and cell loss factor (0) in lung cancer. Neither bcl-2 nor p53 overexpression was associated with high cell loss but strong bcl-2 staining was associated with higher TLI. Concomitant strong p53 and bcl-2 expression, not the usual inverse relationship, plus high cell-loss factor was present in three neuroendocrine carcinomas. Other factors presumably have a role in controlling cell death in these tumours.Keywords: lung carcinoma; neuroendocrine; thymidine labelling index; cell loss factor; bcl-2; p53; apoptosis; immunohistochemistry Apoptosis is a major mechanism of tumour cell loss, itself a major determinant of tumour growth rate, and the protein products of both the p53 and bcl-2 genes have a role in this process. The functions of both these genes and their relationship to other regulatory proteins has been extensively reviewed (Lane, 1993;Piepentol and Vogelstein, 1993;Lu et al, 1996;Yang and Korsmeyer, 1996).In an earlier study (Kerr and Lamb, 1984), we derived data on cell proliferation and cell loss in 17 human lung tumours. Tumour thymidine labelling index (TLI) was measured in vitro and, using recognized methods and formulae (Collins et al, 1956; Steel 1967), a cell loss factor (0) was calculated. Cell loss factor expresses the proportion of cells being lost from a population relative to the number being added to it by mitotic activity. Thus a cell loss factor of 0.90 implies that for every 100 cells being added to a population by mitosis, 90 are lost by whatever means.In this study, we investigate the relationship between the cell proliferation/loss data in these 17 human lung tumours and their expression of the p53 and bcl-2 genes assessed immunohistochemically. MATERIALS AND METHODSThe histological classification of the tumours required revision of the original (Kerr and Lamb, 1984). 'Large-cell carcinoma with stratification' is now classified as poorly differentiated squamous carcinoma. One tumour, thought originally to be a metastatic deposit of large-cell carcinoma, now, with follow-up, fulfils criteria of a primary large-cell neuroendocrine cancer. Thirteen of the cases were typical bronchogenic carcinomas (six squamous, three adenocarcinoma, two small-cell undifferentiated, one largecell neuroendocrine and one large-cell undifferentiated carcinoma). Two were renal cell carcinoma metastases and one a primary clear cell carcinoma.Tumour thymidine labelling index (TLI) was obtained by in vitro incubation of tumour fragments with tritiated thymidine

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“…T, =/-Ts SPF from this follows: including breast cancer, gastrointestinal and haematological malignancies (Hall and Levison, 1992 Table II) an estimate of the cell loss factor in individual cases u-vas made in the present study. The cell loss factor varied from 35°o to 990o with a median of 88%.…”

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confidence: 94%

Growth rate of lung metastases and S-phase fraction as determined by flow cytometry from the primary tumour in 25 patients with bone or soft-tissue sarcomas

Blomqvist

Huuhtanen²,

Pan³

et al. 1996

Br J Cancer

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Review: assessment of cell proliferation in histological material.

Cited by 414 publications

References 146 publications

Ki-67 expression and patients survival in lung cancer: systematic review of the literature with meta-analysis

Ki-67 expression and patients survival in lung cancer: systematic review of the literature with meta-analysis

Cell proliferation, cell loss and expression of bcl-2 and p53 in human pulmonary neoplasms

Growth rate of lung metastases and S-phase fraction as determined by flow cytometry from the primary tumour in 25 patients with bone or soft-tissue sarcomas

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