Review article: new insights into the pathogenesis of radiation‐induced intestinal dysfunction

Wk, MacNaughton

doi:10.1046/j.1365-2036.2000.00745.x

Cited by 115 publications

(64 citation statements)

References 44 publications

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“…Second, mucosal denudation is merely one of several events underlying clinical symptoms related to cytotoxic treatment. [13][14][15][16] Comparison of the citrulline assay results with the SPT results yielded some striking differences between both endpoints. The citrulline level (mol/L or % baseline) did not correlate with gut permeability (the L/R ratio).…”

Section: Discussionmentioning

confidence: 99%

Monitoring myeloablative therapy‐induced small bowel toxicity by serum citrulline concentration

Lutgens

Blijlevens

Deutz

et al. 2004

Cancer

140

107

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BACKGROUNDIntestinal mucositis is an important cause of cancer treatment‐related morbidity and mortality, carrying a serious economic burden. Currently, objective parameters are lacking that would enable the monitoring of gut damage in routine clinical practice, thus hindering the development of clinical studies designed to investigate potential new strategies aimed at reducing or preventing this side effect. The authors investigated the characteristics of serum citrulline concentration compared with sugar permeability tests with respect to its use as a marker for cancer treatment‐induced small bowel injury.METHODSIn this prospective study, 10 patients with hematologic malignancies who were receiving myeloablative therapy had gut toxicity assessed with sugar permeability tests. Serum citrulline concentrations also were determined using archival serum samples. The association between both parameters and their respective characteristics were analyzed and compared with data from the literature.RESULTSSensitivity and specificity were better for the citrulline assay compared with sugar permeability tests. Maximum gut damage assessed with the citrulline assay was observed 1–2 weeks earlier compared with the sugar permeability test. Similarly, citrulline indicated recovery of gut damage at 3 weeks after transplantation, whereas most sugar permeability tests remained abnormal.CONCLUSIONSThe simplicity of the method, the low costs, and the lack of drawbacks to the method make the citrulline assay the first choice for measuring and monitoring treatment‐related gut damage and provides an objective parameter for cancer treatment‐related gut toxicity. Cancer 2005. © 2004 American Cancer Society.

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Section: Discussionmentioning

confidence: 99%

Monitoring myeloablative therapy‐induced small bowel toxicity by serum citrulline concentration

Lutgens

Blijlevens

Deutz

et al. 2004

Cancer

140

107

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“…For patients with pelvic cancer, a serious complication of radiotherapy is the radiation injury to small intestinal epithelium [6] . Small intestinal epithelium contains four major cell types: columnar cells, goblet cells, stem cells, and Paneth cells [7] . Intestinal stem cells, which are most sensitive to ionizing radiation, are located in the crypt portion of the intestine.…”

Section: Introductionmentioning

confidence: 99%

Differentially expressed proteins of gamma-ray irradiated mouse intestinal epithelial cells by two-dimensional electrophoresis and MALDI-TOF mass spectrometry

Zhang

Su²,

Ai³

et al. 2003

WJG

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AIM:To identify the differentially expressed proteins involved in ionizing radiation in mice and to explore new ways for studying radiation-related proteins. METHODS:Bal B/c mice grouped as sham-irradiation, 3 h and 72 h irradiation were exposed to 9.0Gy single dose of γ-irradiation. Intestinal epithelia were isolated from mice, and total proteins were extracted with urea containing solution. A series of methods were used, including twodimensional electrophoresis, PDQuest 2-DE software analysis, peptide mass fingerprinting based on matrixassisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and SWISS-PROT database searching, to separate and identify the differential proteins. Western blotting and RT-PCR were used to validate the differentially expressed proteins. RESULTS:Mouse intestine was severely damaged by 9.0 Gy γ-irradiation. Image analysis of two-dimensional gels revealed that averages of 638±39, 566±32 and 591±29 protein spots were detected in 3 groups, respectively, and the majority of these protein spots were matched. About 360 protein spots were matched between normal group and 3 h irradiation group, and the correlation coefficient was 0.78 by correlation analysis of gels. Also 312 protein spots matched between normal group and 72 h irradiation group, and 282 protein spots between 3 h and 72 h irradiation groups. Twenty-eight differential protein spots were isolated from gels, digested with trypsin, and measured with MALDI-TOF-MS. A total of 25 spots yielded good spectra, and 19 spots matched known proteins after database searching. These proteins were mainly involved in anti-oxidation, metabolism, signal transduction, and protein post-translational processes. Western-blotting confirmed that enolase was up-regulated by γ-irradiation. Upregulation of peroxiredoxin I was verified by applying RT-PCR technique, but no change occurred in Q8VC72. CONCLUSION:These differentially expressed proteins might play important roles when mouse intestine was severely injured by γ-irradiation. It is suggested that differential proteomic analysis may be a useful tool to study the proteins involved in radiation damage of mouse intestinal epithelia.

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“…Las lesiones agudas suelen deberse al daño epitelial (9), los trastornos motores originados por la irritación neural y otras anomalías provocadas por la radiación (10,11). La sintomatología suele aparecer a los pocos días o semanas de la exposición.…”

Section: Discussionunclassified

Obscure gastrointestinal bleeding: a complication of radiation enteritis diagnosed by wireless capsule endoscopy

Ares

Conde

Ruzo

et al. 2004

Rev. esp. enferm. dig.

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Obscure gastrointestinal bleeding is a common disorder and may account for as many as 5% of all gastrointestinal hemorrhages. It is often caused by lesions in the small intestine, which were very complicated to examine prior to the advent of wireless capsule endoscopy.Here we present the case of a 31-year-old woman with obscure gastrointestinal bleeding as a complication of radiation enteritis, which was diagnosed only after she underwent an examination with wireless capsule endoscopy.This technique has proven to be far superior to other radiographic and endoscopic methods in diagnosing obscure gastrointestinal bleeding and pathologies of the small intestine in general.

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Review article: new insights into the pathogenesis of radiation‐induced intestinal dysfunction

Cited by 115 publications

References 44 publications

Monitoring myeloablative therapy‐induced small bowel toxicity by serum citrulline concentration

Monitoring myeloablative therapy‐induced small bowel toxicity by serum citrulline concentration

Differentially expressed proteins of gamma-ray irradiated mouse intestinal epithelial cells by two-dimensional electrophoresis and MALDI-TOF mass spectrometry

Obscure gastrointestinal bleeding: a complication of radiation enteritis diagnosed by wireless capsule endoscopy

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