Review and Meta‐Analysis: SARS‐CoV‐2 and Enveloped Virus Detection in Feces and Wastewater

Twigg, Charlotte; Wenk, Jannis

doi:10.1002/cben.202100039

Cited by 11 publications

(20 citation statements)

References 162 publications

(141 reference statements)

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“…Viruses exist in various shapes and sizes, ranging between 10-400 nm. Furthermore, differences exist in genome structure, chemical composition, reproduction, and range of host species (Twigg & Wenk, 2022). Viruses are composed of proteins, carbohydrates and lipids (Twigg & Wenk, 2022).…”

Section: Coronavirusesmentioning

confidence: 99%

“…Furthermore, differences exist in genome structure, chemical composition, reproduction, and range of host species (Twigg & Wenk, 2022). Viruses are composed of proteins, carbohydrates and lipids (Twigg & Wenk, 2022). They have a virus-coded protein capsid that surrounds the nucleic acid.…”

Section: Coronavirusesmentioning

confidence: 99%

“…They have a virus-coded protein capsid that surrounds the nucleic acid. The capsid is responsible for host cell recognition and binding mechanisms (Twigg & Wenk, 2022).…”

Section: Coronavirusesmentioning

confidence: 99%

“…Previous research on the removal and inactivation of viruses in wastewater treatment plants (WWTP) has focused on non-enveloped enteric viruses as they are abundant in wastewater and readily transmitted via the fecal-oral route (Twigg & Wenk, 2022). However, coronaviruses are enveloped viruses that are airborne and primarily transmitted via respiratory droplets.…”

Section: Sars-cov-2 In Wastewatermentioning

confidence: 99%

“…However, coronaviruses are enveloped viruses that are airborne and primarily transmitted via respiratory droplets. But besides detection of SARS-CoV-2 in an infected individual's saliva and nasal secretion, viral RNA has also been detected in feces and urine (Ahmed et al, 2022;Twigg & Wenk, 2022). The load of SARS-CoV-2 in feces of infected patients, symptomatic or asymptomatic, varies for each individual but can be in the range of 10 4 -10 8 copies per liter (Twigg & Wenk, 2022;Zhang et al, 2022).…”

Section: Sars-cov-2 In Wastewatermentioning

confidence: 99%

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Wastewater Surveillance of SARS-CoV-2 at a Canadian University Campus and the Impact of Wastewater Characteristics on Viral RNA Detection.

Bitter¹

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SARS-CoV-2 levels in the wastewater of a Canadian university campus and their residence buildings were monitored to identify changes, peaks, and hotspots of COVID-19 transmission and search for associations with campus events, social gatherings, long weekends, and holidays.Wastewater signals largely correlated with clinically confirmed cases, often increased following long weekends, and decreased after the implementation of lockdowns. Furthermore, the impact of wastewater parameters on SARS-CoV-2 detection was investigated, and the efficiency of ultrafiltration and centrifugation concentration methods were compared. Results indicated more sensitive results with the centrifugation method for wastewater with high solids content and with the ultrafiltration method for low solids content. Wastewater characteristics from the building sewers were more variable than overall campus wastewater. Statistical analysis was performed to manifest the observations. Overall, wastewater surveillance provided actionable information and was able to bring high-risk factors and events to the attention of the decision-makers, enabling timely corrective measures. ACKNOWLEDGEMENTI would like to first and foremost thank my supervisor Dr. Banu Ormeci for her support, guidance, and knowledge throughout the entire Master's. I would also like to thank the research team involved in the wastewater surveillance project, especially Richard Kibbee, who has taught me many laboratory procedures and provided continued guidance. Additionally, I like to thank my family and friends that supported me throughout the entire two years of my graduate studies. I am also very grateful for the support of the University administration in this project. Last but not least, I thank the Ontario Ministry of Environment, Conservation and Parks for financial support provided to the project. The views expressed in this thesis are the views of the author and do not necessarily reflect those of the Province of Ontario.

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Section: Coronavirusesmentioning

confidence: 99%

Section: Coronavirusesmentioning

confidence: 99%

“…They have a virus-coded protein capsid that surrounds the nucleic acid. The capsid is responsible for host cell recognition and binding mechanisms (Twigg & Wenk, 2022).…”

Section: Coronavirusesmentioning

confidence: 99%

Section: Sars-cov-2 In Wastewatermentioning

confidence: 99%

Section: Sars-cov-2 In Wastewatermentioning

confidence: 99%

See 3 more Smart Citations

Wastewater Surveillance of SARS-CoV-2 at a Canadian University Campus and the Impact of Wastewater Characteristics on Viral RNA Detection.

Bitter¹

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Implementing wastewater surveillance for SARS‐CoV‐2 on a university campus: Lessons learned

Wartell

Proano

Bakalian

et al. 2022

Water Environment Research

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Wastewater surveillance, also known as wastewater‐based epidemiology (WBE), has been successfully used to detect SARS‐CoV‐2 and other viruses in sewage in many locations in the United States and globally. This includes implementation of the surveillance on college and university campuses. A two‐phase study was conducted during the 2020–2021 academic year to test the feasibility of a WBE system on campus and to supplement the clinical COVID‐19 testing performed for the student, staff, and faculty body. The primary objective during the Fall 2020 semester was to monitor a large portion of the on‐campus population and to obtain an understanding of the spreading of the SARS‐CoV‐2 virus. The Spring 2021 objective was focused on selected residence halls and groups of residents on campus, as this was more efficient and relevant for an effective follow‐up response. Logistical problems and planning oversights initially occurred but were corrected with improved communication and experience. Many lessons were learned, including effective mapping, site planning, communication, personnel organization, and equipment management, and obtained along the way, thereby paving an opportune guide for future planning efforts. Practitioner Points WBE was successful in the detection of many SARS‐CoV‐2 variants incl. Alpha, Beta, Gamma, Delta, Lambda, Mu, and Omicron. Careful planning and contingencies were essential for a successful implementation of a SARS‐CoV‐2 monitoring program. A surveillance program may be important for detection and monitoring of other public health relevant targets in wastewater incl. bacteria, viruses, fungi and viruses. Diverse lessons were learned incl. effective mapping, site planning, communication, personnel organization, and equipment management, thereby providing a guide for future planning efforts.

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Wastewater-based prediction of COVID-19 cases using a highly sensitive SARS-CoV-2 RNA detection method combined with mathematical modeling

Ando

Murakami

Ahmed

et al. 2023

Environment International

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Review and Meta‐Analysis: SARS‐CoV‐2 and Enveloped Virus Detection in Feces and Wastewater

Cited by 11 publications

References 162 publications

Wastewater Surveillance of SARS-CoV-2 at a Canadian University Campus and the Impact of Wastewater Characteristics on Viral RNA Detection.

Wastewater Surveillance of SARS-CoV-2 at a Canadian University Campus and the Impact of Wastewater Characteristics on Viral RNA Detection.

Implementing wastewater surveillance for SARS‐CoV‐2 on a university campus: Lessons learned

Wastewater-based prediction of COVID-19 cases using a highly sensitive SARS-CoV-2 RNA detection method combined with mathematical modeling

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