Reversion of the ELISPOT test after treatment in Gambian tuberculosis cases

Aiken, Alexander M.; Hill, Philip C.; Fox, Annette; McAdam, Keith P. W. J.; Jackson-Sillah, Dolly; Lugos, Moses D.; Donkor, Simon; Adegbola, Richard A.; Brookes, Roger H.

doi:10.1186/1471-2334-6-66

Cited by 112 publications

(97 citation statements)

References 15 publications

(14 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A correlation between antigen load and the extent of IFN-␥ secretion in response to ESAT-6 and CFP-10 has been observed both in experimental systems and in humans (33)(34)(35). Further evidence in favor of this relationship comes from observations that demonstrate a falling response during successful chemotherapeutic treatment (3,33,36). The bacillary burden in LTBI is many logs lower than in active disease and so it may not be possible to conclude with confidence that a negative IFNGRA excludes LTBI.…”

Section: Discussionmentioning

confidence: 91%

Effect of HIV-1 Infection on T-Cell–based and Skin Test Detection of Tuberculosis Infection

Rangaka¹,

Wilkinson²,

Seldon³

et al. 2007

Am J Respir Crit Care Med

185

152

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 91%

Effect of HIV-1 Infection on T-Cell–based and Skin Test Detection of Tuberculosis Infection

Rangaka¹,

Wilkinson²,

Seldon³

et al. 2007

Am J Respir Crit Care Med

185

152

View full text Add to dashboard Cite

“…This molecule also exists in bacillus Calmette-Guerin (BCG) vaccine, with expression quantity 10 times higher than that of BCG [3] . The antigen contains seven antigenic epitopes and can induce humoral and cellular immune responses and be used for serological diagnosis of TB.…”

Section: Relevant Antigens Of Humoral Immunity Against Mtbmentioning

confidence: 99%

“…As a fibronectin-binding protein, Ag85 exists in higher amounts of serum of patients with active TB in comparison with patients with non-active TB or other lung diseases or healthy controls [2] . In the study among indigenous people of Mexico, Abebe et al [3] proved that IgG exhibits good effect on Mtb complex Ag85. Another study showed that compared with patients cured by anti-TB treatment, effective antibody titer was more remarkable for Ag85 in non-cavernous TB.…”

Section: Relevant Antigens Of Humoral Immunity Against Mtbmentioning

confidence: 99%

See 1 more Smart Citation

Research progress on immune response of B lymphocytes and anti-Mycobacterium tuberculosis infection

You¹

2016

Infection International

View full text Add to dashboard Cite

Multiple studies elucidated the importance of cellular immune mechanisms for protection against Mycobacterium tuberculosis. However, recent studies showed that B lymphocytes play a role that is underestimated through various interactions with cellular immune response, forming an important aspect of host defense against M. tuberculosis bacteria. Therefore, the author hereby proposes a progressive perspective for immunology of tuberculosis, i.e., cellular immunity and humoral immunity are not necessarily mutually exclusive. The present study summarizes recent studies that support the important role of B lymphocytes in terms of M. tuberculosis infection.Mycobacterium tuberculosis (Mtb) belong to Mycobacterium genus. This pathogenic species causes tuberculosis (TB). TB remains an important infectious disease nowadays. This disease can invade body organs, with pulmonary TB being the most commonly observed. Humoral immunity plays a negligible role in the defense against Mtb. However, antibodies were reported to pose effects on Mtb since the late 19th century. Humoral immunity indicates that under stimulations from antigens, as represented by B lymphocytes, i m mu noc y te s i n t he i m mu ne s y s tem of orga n i sm s differentiate and proliferate into plasma cells and synthesize various types of antibodies to generate specific immune responses with biological effects. Adoptively transferred B lymphocytes can limit deterioration of inf lammations induced by TB [1] . This condition demonstrates the important role of immunoglobulin-mediated immune regulation. The present study summarizes recent studies on humoral immunity against Mtb.

show abstract

“…On the basis of the hypothesis that the IFN-␥ ELISpot assay could function as a bacterial load sensor, a comparatively smaller amount of SFCs in response to ESAT-6 and/or CFP-10 over time would be indicative of a good response to treatment, leading to a lower probability of relapse. Though encouraging, the readings of recent exploratory studies were confounded by immune status, stage of infection, and previous drug treatment; and no clear conclusion was reached with regard to the predictive value of the ELISpot assay as a marker of therapy response (1,5,13,19,28). Relapse as a clinical trial endpoint is a key issue in drug development.…”

mentioning

confidence: 99%

T Cell Monitoring of Chemotherapy in Experimental Rat Tuberculosis

Foo

Tay

Siew

et al. 2011

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Mycobacterium tuberculosis is the causative agent of a pulmonary epidemic that is estimated to infect one-third of the world's population and that has an increased incidence of multidrug resistance. The evaluation of new chemical entities against M. tuberculosis is hampered by the lack of biological tools to help predict efficacy, from early drug development to clinical trials. As the rat is the animal species of choice in the pharmaceutical industry, we have developed a rat model of acute and chronic phases of M. tuberculosis infection for drug efficacy testing. In this model, we have evaluated the impact of tuberculosis drugs on T cell response using the enzyme-linked immunospot assay methodology. Infected rats treated with isoniazid (INH) or rifampin (RIF) responded to therapy, the potency of which was comparable to that seen in the mouse. Peripheral blood mononuclear cells from infected rats produced gamma interferon (IFN-␥) in response to RD-1 antigens, such as the 6-kDa early secretory antigen target (ESAT-6) and the 10-kDa culture filtrate protein (CFP-10). A decrease in IFN-␥ spot-forming cells (SFCs) was consistently observed in response to drug treatment. In both the acute-and chronic-phase models, the T cell response was more sensitive to ESAT-6 than to CFP-10. The SFC count in response to ESAT-6 appears to be an indicator of bacterial killing in the rat. Collectively, our data suggest that the ESAT-6 response could be used as a potential surrogate of drug efficacy in the rat and that such a readout could help shorten drug testing during preclinical development.Mycobacterium tuberculosis infection is among the world's leading infectious diseases, causing about 2 million deaths annually. The emergence of multidrug-resistant M. tuberculosis strains along with the increase of HIV coinfected cases worsens the situation (42). In countries with a high incidence of tuberculosis (TB), TB control programs rely on a diagnostic methods and drugs that have been developed decades ago and that are inadequate to effectively control the epidemic. The urgent need to develop new diagnostic tools as well as new therapeutic interventions is hampered by long clinical trials, where markers of infection and drug response are lacking (38).For decades, the tuberculin skin test (TST) has been used to diagnose TB (18). The TST measures cell-mediated immunity in the form of a delayed-type hypersensitivity response to the purified protein derivative (PPD), a crude mixture of antigens shared among M. tuberculosis, Mycobacterium bovis BCG, and several nontuberculous mycobacteria (NTM). As a result, the TST has lower specificity in populations with high BCG coverage and NTM exposure and shows poor sensitivity in immunocompromised individuals (30). The gamma interferon (IFN-␥) enzyme-linked immunospot (ELISpot) assay has emerged as an alternative to the TST. The assay consists of in vitro stimulation of peripheral blood mononuclear cells (PBMCs) using RD-1 antigens, the 6-kDa early secretory antigen target (ESAT-6) and the 10-kDa cul...

show abstract

Reversion of the ELISPOT test after treatment in Gambian tuberculosis cases

Cited by 112 publications

References 15 publications

Effect of HIV-1 Infection on T-Cell–based and Skin Test Detection of Tuberculosis Infection

Effect of HIV-1 Infection on T-Cell–based and Skin Test Detection of Tuberculosis Infection

Research progress on immune response of B lymphocytes and anti-Mycobacterium tuberculosis infection

T Cell Monitoring of Chemotherapy in Experimental Rat Tuberculosis

Contact Info

Product

Resources

About