Reversibly Stabilized Multifunctional Dextran Nanoparticles Efficiently Deliver Doxorubicin into the Nuclei of Cancer Cells

Li, Yuling; Zhu, Li; Liu, Zhaozhong; Cheng, Ru; Meng, Fenghua; Cui, Jie; Ji, Shun‐Jun; Zhong, Zhiyuan

doi:10.1002/ange.200904260

Cited by 66 publications

(53 citation statements)

References 47 publications

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“…For the method to be accurate, we considered the contribution to the absorbance of sources other than variations in drug concentration (mainly the presence of Pluronic ® F-68 [Sigma-Aldrich] and PBCA degradation products in the supernatant solutions) by subtracting the absorbance of the supernatant produced under the same conditions but without DOX. [21][22][23] DOX incorporation to the PBCA NPs was expressed in terms of drug entrapment efficiency (%) and drug loading (%). These parameters were calculated as follows: …”

Section: Characterization Methodsmentioning

confidence: 99%

Enhanced antitumor activity of doxorubicin in breast cancer through the use of poly(butylcyanoacrylate) nanoparticles

Cabeza¹,

Ortíz²,

Arias³

et al. 2015

IJN

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The use of doxorubicin (DOX), one of the most effective antitumor molecules in the treatment of metastatic breast cancer, is limited by its low tumor selectivity and its severe side effects. Colloidal carriers based on biodegradable poly(butylcyanoacrylate) nanoparticles (PBCA NPs) may enhance DOX antitumor activity against breast cancer cells, thus allowing a reduction of the effective dose required for antitumor activity and consequently the level of associated toxicity. DOX loading onto PBCA NPs was investigated in this work via both drug entrapment and surface adsorption. Cytotoxicity assays with DOX-loaded NPs were performed in vitro using breast tumor cell lines (MCF-7 human and E0771 mouse cancer cells), and in vivo evaluating antitumor activity in immunocompetent C57BL/6 mice. The entrapment method yielded greater drug loading values and a controlled drug release profile. Neither in vitro nor in vivo cytotoxicity was observed for blank NPs. The 50% inhibitory concentration (IC 50 ) of DOX-loaded PBCA NPs was significantly lower for MCF-7 and E0771 cancer cells (4 and 15 times, respectively) compared with free DOX. Furthermore, DOX-loaded PBCA NPs produced a tumor growth inhibition that was 40% greater than that observed with free DOX, thus reducing DOX toxicity during treatment. These results suggest that DOX-loaded PBCA NPs have great potential for improving the efficacy of DOX therapy against advanced breast cancers.

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Section: Characterization Methodsmentioning

confidence: 99%

Enhanced antitumor activity of doxorubicin in breast cancer through the use of poly(butylcyanoacrylate) nanoparticles

Cabeza¹,

Ortíz²,

Arias³

et al. 2015

IJN

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show abstract

“…The terminal 1,2-dithiolanes in PEG-a II and PEGb II compounds were introduced due to their fast and tunable disulfide exchange properties, which can produce disulfide clusters through multiple cross-linking interactions. 23,24 Functionalization of Na-alg with PEG Derivatives. The alg backbone presents a distribution of carboxyl and hydroxyl functionalities available for chemical functionalization.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Synthesis Strategies to Extend the Variety of Alginate-Based Hybrid Hydrogels for Cell Microencapsulation

et al. 2017

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The production of hydrogel microspheres (MS) for cell immobilization, maintaining the favorable properties of alginate gels but presenting enhanced performance in terms of in vivo durability and physical properties, is desirable to extend the therapeutic potential of cell transplantation. A novel type of hydrogel MS was produced by straightforward functionalization of sodium alginate (Na-alg) with heterotelechelic poly(ethylene glycol) (PEG) derivatives equipped with either end thiol or 1,2-dithiolane moieties. Activation of the hydroxyl moieties of the alginate backbone in the form of imidazolide intermediate allowed for fast conjugation to PEG oligomers through a covalent carbamate linkage. Evaluation of the modified alginates for the preparation of MS combining fast ionic gelation ability of the alginate carboxylate groups and slow covalent cross-linking provided by the PEG-end functionalities highlighted the influence of the chemical composition of the PEG-grafting units on the physical characteristics of the MS. The mechanical properties of the MS (resistance and shape recovery) and durability of PEG-grafted alginates in physiological environment can be adjusted by varying the nature of the end functionalities and the length of the PEG chains. In vitro cell microencapsulation studies and preliminary in vivo assessment suggested the potential of these hydrogels for cell transplantation applications. ■ INTRODUCTIONProgress in therapies relying on the allo-or xenotransplantation of immobilized cells and tissue strongly depends on the quality of the immobilizing material. Currently, the translation of related therapies to the clinics, for example to treat end-stage organ failure and end-stage diseases such as cancer, diabetes mellitus, and acute liver failure, 1,2 is hindered by the lack of materials having the perfect properties. Hydrogels prepared from the biopolymer sodium alginate (Na-alg) or derivatives of it have been reported in abundant papers as particularly advantageous because they fulfill general requirements such as the spontaneous formations of hydrogels in the presence of divalent cations (e.g., Ca 2+ , Ba 2+ ) under mild conditions of temperature and pH, and high biocompatibility. 3−6 Maintaining these advantages but overcoming several drawbacks, including the lack of in vivo mechanical resistance and stability, and defects in permselectivity, 7,8 are the focus of current research. There are additional limitations caused by the dimension of the targeted final application. This calls in particular for therapies which intend the transplantation of allo-or xenogeneic cells immobilized in microspheres (MS) in order to allow for miniinvasive surgery. 9,10 The stabilization of alginate gel beads by coating the MS with polycations such as (poly(L-lysine), poly(L-ornithine), and poly(L-guanidine) was investigated, but impaired cell graft function in vivo. 11 Another strategy to improve the performance of alginate MS relies on the combination with other polymers such as poly(ethylene glycol) (P...

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“…60 For example, dextran nanoparticles have been designed for the delivery of doxorubicin into the nuclei of cancer cells as intelligent drug delivery systems in chemotherapy. 64 …”

Section: Dextranmentioning

confidence: 99%

Natural Polysaccharide based Nanoparticles for Drug/Gene Delivery

Salatin¹,

Jelvehgari²

2017

Pharm Sci

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Reversibly Stabilized Multifunctional Dextran Nanoparticles Efficiently Deliver Doxorubicin into the Nuclei of Cancer Cells

Cited by 66 publications

References 47 publications

Enhanced antitumor activity of doxorubicin in breast cancer through the use of poly(butylcyanoacrylate) nanoparticles

Enhanced antitumor activity of doxorubicin in breast cancer through the use of poly(butylcyanoacrylate) nanoparticles

Synthesis Strategies to Extend the Variety of Alginate-Based Hybrid Hydrogels for Cell Microencapsulation

Natural Polysaccharide based Nanoparticles for Drug/Gene Delivery

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Reversibly Stabilized Multifunctional Dextran Nanoparticles Efficiently Deliver Doxorubicin into the Nuclei of Cancer Cells

Cited by 66 publications

References 47 publications

Enhanced antitumor activity of doxorubicin in breast cancer through the use of poly(butylcyanoacrylate) nanoparticles

Enhanced antitumor activity of doxorubicin in breast cancer through the use of poly(butylcyanoacrylate) nanoparticles

Synthesis Strategies to Extend the Variety of Alginate-Based Hybrid Hydrogels for Cell Microencapsulation

Natural Polysaccharide based Nanoparticles for Drug/Gene Deliv­ery

Contact Info

Product

Resources

About

Natural Polysaccharide based Nanoparticles for Drug/Gene Delivery