Reversibly Photo‐Modulating Mechanical Stiffness and Toughness of Bioengineered Protein Fibers

Xiao

et al. 2021

Angew Chem Int Ed

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Genetically Engineered Polypeptide Adhesive Coacervates for Surgical Applications

Xiao

et al. 2021

Angew Chem Int Ed

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“…These SUPs were produced by recombinant DNAtechnology and expressed in E. Coli. [20][21][22] Aseries of SUPs were produced with different chain lengths and charges,including K18, K72, K108, E36, E72, and E144. Thel etter code denotes positive (K) or negative (E) variants while the digit indicates the number of charged amino acid residues (Figures 1A and S1-S2, Table S1).…”

Section: Resultsmentioning

confidence: 99%

Genetically Engineered Polypeptide Adhesive Coacervates for Surgical Applications

Xiao

et al. 2021

Angewandte Chemie

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Adhesive hydrogels have been developed for wound healing applications.H owever,t heir adhesive performance is impaired dramatically due to their high swelling on wet tissues. To tackle this challenge,w ef abricated an ew type of nonswelling protein adhesive for underwater and in vivo applications.I nt his soft material, the electrostatic complexation between supercharged polypeptides with oppositely charged surfactants containing 3,4-dihydroxylphenylalanine or azobenzenemoieties playsanimportant role for the formation of ultra-strong adhesive coacervates.R emarkably,t he adhesion capability is superior to commercial cyanoacrylate when tested in ambient conditions.Moreover,the adhesion is stronger than other reported protein-based adhesives in underwater environment. The ex vivo and in vivo experiments demonstrate the persistent adhesive performance and outstanding behaviors for wound sealing and healing.

“…by DNA recombinant technology (Figure 3A). [42][43][44][45][46] The high net charge of SUPs is encoded in the pentapeptide repeat unit (VPGXG) n in which X is substituted by a lysine residue (K) or a glutamic acid (E). Therefore, the highly charged SUPs allow them electrostatically complexed with opposite charged surfactants, resulting in the formation of coacervate-based biomaterials.…”

Section: Elp-surfactant Adhesivesmentioning

confidence: 99%

Bioengineered Protein‐based Adhesives for Biomedical Applications

Han

Wang

et al. 2021

Chemistry A European J

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Protein-based adhesives with their robust adhesion performance and excellent biocompatibility have been extensively explored over years. In particular, the unique adhesion behaviours of mussel and sandcastle worm inspired the development of synthetic adhesives. However, the chemical synthesized adhesives often demonstrate weak underwater adhesion performance and poor biocompatibility/biodegradability, limiting their further biomedical applications. In sharp contrast, genetically engineering endows the protein-based adhesives the ability to maintain underwater adhesion property as well as biocompatibility/biodegradability. Herein, we outline recent advances in the design and development of protein-based adhesives by genetic engineering. We summarize the fabrication and adhesion performance of elastin-like polypeptide-based adhesives, followed by mussel foot protein (mfp) based adhesives and other sources protein-based adhesives, such as, spider silk spidroin and suckerin. In addition, the biomedical applications of these bioengineered protein-based adhesives are presented. Finally, we give a brief summary and perspective on the future development of bioengineered protein-based adhesives.