Abstract:Single cell measurements with living specimen like, for example, the ciliated protozoan Paramecium caudatum can be a challenging task. We present here a microfluidic trapping mechanism for measurements with these micro-organisms that can be used, e.g., for optical measurements to correlate cellular functions with the phase state of the lipid membrane. Here, we reversibly trap single cells in small compartments. Furthermore, we track and analyze the swimming behavior of single cells over several minutes. Before… Show more
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